全文获取类型
收费全文 | 8931篇 |
免费 | 595篇 |
国内免费 | 46篇 |
专业分类
耳鼻咽喉 | 173篇 |
儿科学 | 266篇 |
妇产科学 | 136篇 |
基础医学 | 1108篇 |
口腔科学 | 117篇 |
临床医学 | 780篇 |
内科学 | 2432篇 |
皮肤病学 | 140篇 |
神经病学 | 781篇 |
特种医学 | 376篇 |
外国民族医学 | 7篇 |
外科学 | 1424篇 |
综合类 | 45篇 |
一般理论 | 1篇 |
预防医学 | 580篇 |
眼科学 | 78篇 |
药学 | 559篇 |
中国医学 | 9篇 |
肿瘤学 | 560篇 |
出版年
2023年 | 45篇 |
2022年 | 62篇 |
2021年 | 182篇 |
2020年 | 109篇 |
2019年 | 185篇 |
2018年 | 253篇 |
2017年 | 169篇 |
2016年 | 176篇 |
2015年 | 260篇 |
2014年 | 288篇 |
2013年 | 387篇 |
2012年 | 572篇 |
2011年 | 564篇 |
2010年 | 308篇 |
2009年 | 303篇 |
2008年 | 487篇 |
2007年 | 491篇 |
2006年 | 572篇 |
2005年 | 522篇 |
2004年 | 447篇 |
2003年 | 423篇 |
2002年 | 373篇 |
2001年 | 197篇 |
2000年 | 223篇 |
1999年 | 187篇 |
1998年 | 97篇 |
1997年 | 68篇 |
1996年 | 80篇 |
1995年 | 52篇 |
1994年 | 53篇 |
1993年 | 47篇 |
1992年 | 117篇 |
1991年 | 112篇 |
1990年 | 91篇 |
1989年 | 96篇 |
1988年 | 68篇 |
1987年 | 97篇 |
1986年 | 79篇 |
1985年 | 85篇 |
1984年 | 57篇 |
1983年 | 62篇 |
1982年 | 29篇 |
1981年 | 29篇 |
1979年 | 65篇 |
1977年 | 30篇 |
1976年 | 29篇 |
1974年 | 36篇 |
1973年 | 38篇 |
1971年 | 36篇 |
1970年 | 27篇 |
排序方式: 共有9572条查询结果,搜索用时 31 毫秒
91.
Magali Bouhours Sandrine Luce Damien Sternberg Jean Claude Willer Bertrand Fontaine Nacira Tabti 《The Journal of physiology》2005,565(2):415-427
Missense mutations in the human skeletal muscle Na+ channel α subunit (hSkM1) are responsible for a number of muscle excitability disorders. Among them, paramyotonia congenita (PC) is characterized by episodes of muscle stiffness induced by cold and aggravated by exercise. We have identified a new PC-associated mutation, which substitutes aspartic acid for a conserved alanine in the S4–S5 linker of domain III (A1152D). This residue is of particular interest since its homologue in the rat brain type II Na+ channel has been suggested as an essential receptor site for the fast inactivation particle. To identify the biophysical changes induced by the A1152D mutation, we stably expressed hSkM1 mutant or wild-type (WT) channels in HEK293 (human embryonic kidney) cells, and recorded whole-cell Na+ currents with the patch-clamp technique. Experiments were performed both at 21 and 11°C to better understand the sensitivity to cold of paramyotonia. The A1152D mutation disrupted channel fast inactivation. In comparison to the WT, mutant channels inactivated with slower kinetics and displayed a 5 mV depolarizing shift in the voltage dependence of the steady-state. The other noticeable defect of A1152D mutant channels was an accelerated rate of deactivation from the inactivated state. Decreasing temperature by 10°C amplified the differences in channel gating kinetics between mutant and WT, and unveiled differences in both the sustained current and channel deactivation from the open state. Overall, cold-exacerbated mutant defects may result in a sufficient excess of Na+ influx to produce repetitive firing and myotonia. In the light of previous reports, our data point to functional as well as phenotypic differences between mutations of conserved S4–S5 residues in domains II and III of the human skeletal muscle Na+ channel. 相似文献
92.
P Gautier P Guiraudou F Pezziardi J P Bertrand J P Gagnol 《Journal of cardiovascular pharmacology》1987,9(5):601-610
The intracellular electrophysiologic properties of a new antiarrhythmic substance, penticainide, were studied in isolated rabbit, dog, and guinea pig myocardial preparations superfused or perfused with oxygenated Tyrode's solution. "Therapeutic" concentrations of penticainide (1.5 to 3 X 10(-5) M) had little effect on sinus node automaticity; sinoatrial conduction was slightly delayed. In atrial, Purkinje and ventricular fibers, amplitude, and maximal rate of rise of phase O (dV/dtmax) were decreased by penticainide; Purkinje-ventricle conduction velocity was depressed. Penticainide did not significantly modify action potential duration (APD) of rabbit atria and dog ventricle and reduced APD and effective refractory period (ERP) of dog Purkinje and guinea pig ventricular fibers. Penticainide reduced APD heterogeneity of Purkinje-ventricle junction with a preferential effect at the gate and decreased tension amplitude of perfused papillary muscle in dog heart. The effect of penticainide on dV/dtmax was voltage and rate dependent; the resting block was weak. Thus, penticainide is a class 1 antiarrhythmic agent with properties of class 1B agents such as APD reduction and properties of class 1C agents such as slow recovery kinetic of rate-dependent block. 相似文献
93.
Juliana Chen Solne Bertrand Olivier Galy David Raubenheimer Margaret Allman-Farinelli Corinne Caillaud 《Nutrients》2021,13(5)
The food environment in New Caledonia is undergoing a transition, with movement away from traditional diets towards processed and discretionary foods and beverages. This study aimed to develop an up-to-date food composition database that could be used to analyze food and nutritional intake data of New Caledonian children and adults. Development of this database occurred in three phases: Phase 1, updating and expanding the number of food items to represent current food supply; Phase 2, refining the database items and naming and assigning portion size images for food items; Phase 3, ensuring comprehensive nutrient values for all foods, including saturated fat and total sugar. The final New Caledonian database comprised a total of 972 food items, with 40 associated food categories and 25 nutrient values and 615 items with portion size images. To improve the searchability of the database, the names of 593 food items were shortened and synonyms or alternate spelling were included for 462 foods. Once integrated into a mobile app-based multiple-pass 24-h recall tool, named iRecall.24, this country-specific food composition database would support the assessment of food and nutritional intakes of families in New Caledonia, in a cross-sectional and longitudinal manner, and with translational opportunities for use across the wider Pacific region. 相似文献
94.
95.
96.
M.A. Pajares J.A. Margarit C. García-Camacho J. García-Suarez E. Mateo M. Castaño C. López Forte J. López Menéndez M. Gómez M.J. Soto S. Veiras E. Martín B. Castaño S. López Palanca T. Gabaldón J. Acosta J. Fernández Cruz A.R. Fernández López R. Vicente 《Revista espa?ola de anestesiología y reanimación》2021,68(4):183-231
The ERAS guidelines are intended to identify, disseminate and promote the implementation of the best, scientific evidence-based actions to decrease variability in clinical practice. The implementation of these practices in the global clinical process will promote better outcomes and the shortening of hospital and critical care unit stays, thereby resulting in a reduction in costs and in greater efficiency. After completing a systematic review at each of the points of the perioperative process in cardiac surgery, recommendations have been developed based on the best scientific evidence currently available with the consensus of the scientific societies involved. 相似文献
97.
98.
Yann Gouëffic Jean L. Pin Jean Sabatier Yves Alimi Eric Steinmetz Pierre-Edouard Magnan Olivier Marret Adrien Kaladji Bertrand Chavent Benjamin Kretz Alexandra Jobert Solène Schirr-Bonnans Béatrice Guyomarc'h Valéry P. Riche Lucie S. du Mont Philippe Tessier 《European journal of vascular and endovascular surgery》2021,61(3):447-455
99.
Activation of AMP-activated protein kinase in the liver: a new strategy for the management of metabolic hepatic disorders 总被引:14,自引:1,他引:14
Benoit Viollet Marc Foretz Bruno Guigas Sandrine Horman Renaud Dentin Luc Bertrand Louis Hue Fabrizio Andreelli 《The Journal of physiology》2006,574(1):41-53
It is now becoming evident that the liver has an important role in the control of whole body metabolism of energy nutrients. In this review, we focus on recent findings showing that AMP-activated protein kinase (AMPK) plays a major role in the control of hepatic metabolism. AMPK integrates nutritional and hormonal signals to promote energy balance by switching on catabolic pathways and switching off ATP-consuming pathways, both by short-term effects on phosphorylation of regulatory proteins and by long-term effects on gene expression. Activation of AMPK in the liver leads to the stimulation of fatty acid oxidation and inhibition of lipogenesis, glucose production and protein synthesis. Medical interest in the AMPK system has recently increased with the demonstration that AMPK could mediate some of the effects of the fat cell-derived adiponectin and the antidiabetic drugs metformin and thiazolidinediones. These findings reinforce the idea that pharmacological activation of AMPK may provide, through signalling and metabolic and gene expression effects, a new strategy for the management of metabolic hepatic disorders linked to type 2 diabetes and obesity. 相似文献
100.
M. C. Brown J. N. Larocca C. Sato E. F. Soto J. M. Pasquini 《Molecular and chemical neuropathology / sponsored by the International Society for Neurochemistry and the World Federation of Neurology and research groups on neurochemistry and cerebrospinal fluid》1990,12(3):191-202
In order to find out if the decreased accumulation of cerebroside sulfates observed in 21-d-old undernourished rats was in part the result of an increased rate of catabolism of these galactolipids, the in vivo degradation of brain cerebroside sulfates was studied in 18-d-old normal and undernourished rats. Two hours after the intracranial injection of the precursor (0 time), the animals were injected intraperitoneally with unlabeled sodium sulfate. Labeled cerebroside sulfates were measured in the brain up to 48 h after the chase. In normal animals, the radioactivity decreased at 24 h and 48 h to 55% and 41%, respectively, of the value obtained at 0 time. In undernourished animals, degradation was negligible, since the radioactivity attained at 0 time remained almost constant up to 48 h. The lack of in vivo degradation of cerebroside sulfates observed in the starved rats cannot be explained by a deficiency of Arylsulfatase A, since the pattern of activity of the enzyme was similar in both groups of animals. 相似文献