受体介导的凝血酶对成骨细胞增殖及分化的作用

目的 通过凝血酶对成骨细胞的增殖及分化作用的研究来探讨受体介导的凝血酶的功能.方法 原代成骨细胞分别取自于蛋白酶激活受体(protease-activated receptor,PAR)-1敲除鼠和野生对照鼠的头颅骨.并利用凝血酶,人工合成的PAR-1或PAR-4特异性激活短肽对细胞进行处理,通过对5.溴-2-脱氧尿嘧啶的嵌入及细胞碱性磷酸酶活性的测定探讨PAR-1或PAR-4激活对细胞增殖和分化的影响.结果 在野生鼠成骨细胞,凝血酶及PAR-1激活肽均能促进的细胞增殖和降低碱性磷酸酶的活性,但PAR-4激活肽却无这些作用.然而在PAR-1 敲除鼠的成骨细胞无论是凝血酶还是PAR-4激活肽均不能改变细胞的增殖及碱性磷酸酶的活性.结论 本研究结果 表明凝血酶促进成骨细胞增殖及抑制其分化是通过PAR-1介导的.其他凝血酶受体并不具有此作用.     

Association of insomnia with quality of life, work productivity, and activity impairment

Purpose  To assess the association of insomnia with health-related quality of life (HRQOL), work productivity, and activity impairment. Methods  Data were obtained from the 2005 US National Health and Wellness Survey. Subjects were assigned to the insomnia group (diagnosed insomnia experienced at least a few times a month) or the noninsomnia group (no insomnia or sleep symptoms). HRQOL was assessed using the short form 8 (SF-8) (mental and physical scores). The work productivity and activity impairment questionnaire (WPAI) assessed absenteeism (work time missed), presenteeism (impairment at work), work productivity loss (overall work impairment), and activity impairment. Linear regression models were used to control for potential confounders. Results  A total of 19,711 adults were evaluated (5,161 insomnia, 14,550 noninsomnia). Subjects in the insomnia group had significantly lower SF-8 physical (−5.40) and mental (−4.39) scores and greater activity impairment scores (+18.04) than subjects in the noninsomnia group (P < 0.01 for all). Employed subjects in the insomnia group had greater absenteeism (+6.27), presenteeism (+13.20), and work productivity loss (+10.33) scores than those in the noninsomnia group (P < 0.01 for all). Conclusions  Insomnia is significantly associated with poorer physical and mental quality of life and work productivity loss and activity impairment.     

Mechanisms of hypertension in patients with terminal kidney failure on programmed hemodialysis

The state of some mechanisms involved in AP regulation was studied in 18 patients with terminal renal failure (TRF) on programmed hemodialysis for 7-9 mos. The imbalance of extra- and intracellular sodium, potassium and water causing hypertension, was noted in TRF patients. TRF patients revealed (against a background of the normal activity of plasma renin) a high activity of carboxycathepsin resulting in the creation of conditions for enhanced kinin degradation playing a depressor role and for intense angiotensin II formation, being an important mechanism of persistent hypertension. An increase in the activity of carboxycathepsin can be one of the reasons of hyperaldosteronemia detected in the patients. A single session of hemodialysis does not significantly influence the activity of carboxycathepsin.     

Concentration of tobramycin given by aerosol in the fluid obtained by bronchoalveolar lavage

Whereas previous studies have used only bronchial secretions and sputum, in the present study, bronchoalveolar (BAL) fluid was analysed for tobramycin levels after aerosolization of this antibiotic. In 20 adult patients with a variety of lung disorders, the concentration of tobramycin obtained in the first aliquot of the bronchoalveolar fluid varied from less than 0.1 to 9.2 micrograms ml-1 (mean 2 +/- 2.26 micrograms ml-1) with 18 samples above 0.4 micrograms ml-1. In most of the cases, the concentration of tobramycin achieved values of tobramycin in excess of the minimal inhibitory concentration for most of the microorganisms. Thus, sampling fluids by the bronchoalveolar technique offers a suitable method to study antibiotic levels at the site of broncho-pulmonary infection. These results may help explain why aerosol antibiotic treatment appears to be useful in selected patients, especially in cystic fibrosis patients chronically infected with Pseudomonas aeruginosa.     

Relation between the duration of the chronic phase of chronic myeloid leukemia and total dose of busulfan in the first year of the treatment

The analysis was based on 31 patients with chronic granulocytic leukaemia (CGL) treated during the period 1975-1986 and the correlation between the total dose of busulphan administered during the first year of treatment and the duration of chronic phase of the disease was investigated. We found such correlation and also we found that the duration of the chronic phase was more significantly correlated with one year maintenance dose of busulphan. This suggests that the prognosis in CGL is more connected with the proliferation activity of leukaemic cells than with the extent of disease at the time of diagnosis.     

Klinische Bewertung des Diethanolamins in der Behandlung der Trichophytose beim jungen Mastvieh*/Clinical Evaluation of Diethanolamine in the Treatment of Ringworm in Young Cattle

Zusammenfassung: 106 junge Mastbullen, bei welchen eine Trichophytose festgestellt wurde, wurden mit Diethanolamin in Form einer Salbe behandelt. Nach 14tägiger, 21tägiger und 28tägiger Behandlung wurden jeweils 64,1%, 87,7% and 95,2% der Here als ausgeheilt erkannt. Daraus ist ersichtlich, daß sich Diethanolamin zur Behandlung von mykotischen Erkrankungen eignet. Summary: 106 young bulls with ringworm were treated with an ointment containing diethanolamine. After 14, 21 and 28 days of application of the ointment 64,1%, 87,7% and 95,2% of the animals were cured, respectively. The results of these studies demonstrate the usefulness of diethanolamine in curing mycotic diseases.