Pre-Implantation Multiple Cytokine mRNA Expression Analysis of Donor Lung Grafts Predicts Survival After Lung Transplantation in Humans

While current donor selection with clinical findings is generally effective, the imprecise nature of the assessment forces clinicians to remain on the conservative side. A reliable biological marker would assist donor selection and would improve donor organ utilization. We collected biopsies from 169 donor lungs before implantation. Expression levels of IL-6, IL-8, IL-10, TNF-alpha, IFN-gamma and IL-1beta were measured by quantitative real-time RT-PCR (qRT-PCR). Seventeen cases died within 30 days after transplantation. No donor factor was significantly associated with 30-day mortality. Univariate analysis of the 84 cases for development of the prediction model showed that IL-6, IL-8, TNF-alpha and IL-1beta were risk factors for mortality and IL-10 and IFN-gamma were protective factors. We analyzed the cytokine expression ratios of risk to protective cytokines. A stepwise logistic regression for 30-day mortality demonstrated that a model containing the ratio of IL-6/IL-10 was the most predictive (p = 0.0013). When applied to the remaining 85 cases for validation, the test of model fit was significant (p = 0.039). Using the cytokine ratio, we were able to define three risk groups with striking differences in survival (p = 0.0003). Multi-cytokine analysis of the donor lung graft with qRT-PCR shows significant promise as a strategy to biologically evaluate the donor lung prior to implantation.     

应用吊带方法经皮膀胱颈悬吊治疗女性压力性尿失禁

目的：介绍吊带方法经皮膀胱颈悬吊术治疗女性压力性尿失禁的经验。方法：在近膀胱颈水平，阴道前壁与尿道之间隧道状切口内预置两端带有尼龙线的网状聚丙烯片，术中窥镜监视下，应用Vesica穿刺针经皮穿刺行膀胱颈悬吊治疗女性压力性尿失禁19例。结果：随访3－45个月，平均17个月，除1例逼尿肌反射低下排尿稍费力外，18例患者无尿失禁及排尿困难。结论：吊带方法经皮膀胱颈悬吊术治疗女性压力性尿失禁，操作简便，创伤小，恢复快，临床效果满意。     

更年期妇女生理心理健康状况探讨

目的探讨更年期妇女生理、心理健康状况及相互关系 ,为进一步缓解更年期妇女的症状提供医疗和护理上的依据。方法对北京大学第一临床医院更年期门诊病人进行问卷调查 ,数据结果应用SPSS 8 0数据分析软件包进行处理。结果更年期妇女Kupperman总体平均分为 2 0 12± 9 97分 ,SCL 90评分量表总体平均分为 4 6 6 2±36 2 8分 ,并且以潮热出汗、失眠等躯体症状 ,以及强迫症状、人际关系敏感、抑郁、焦虑等心理症状表现突出。应用激素替代疗法后 ,能有效缓解更年期妇女潮热出汗、抑郁心烦、疲乏、骨关节痛、敌对的症状 ,与未用激素替代疗法者有显著差异。对更年期妇女的生理、心理进行分析显示 ,更年期妇女生理心理症状呈中等程度相关性 (r=0 6 17,P <0 0 0 1)。结论医护人员应加强对更年期妇女生理心理双方面的关注。     

哺乳期营养对室间隔心肌细胞发育的影响——游离心肌细胞的形态测量

在心肌细胞分化性生长时期,改变新生鼠的哺乳期营养条件对这些鼠的空间隔心肌细胞将会产生暂时性作用和持久性影响。哺乳期高营养的暂时性作用是增加空间隔心肌细胞的大小和数量;而低营养的暂时性作用则是减慢室间隔心肌细胞的生长。哺乳期高营养的持久性作用是限制室间隔心肌细胞的过度肥大;而低营养则促进成熟鼠的室间隔心肌细胞的过度肥大。     

下颌骨骨折致颞下颌关节紊乱综征相关因素的临床分析

为分析下颌骨骨折后颞下颌关节发生紊乱综合征的相关因素.按照Helkimo指数对45例下颌骨骨折患者的临床资料进行了回顾性分析.结果显示Helkimo指数的严重程度与下颌骨骨折术后的恢复时间以及下颌骨骨折部位有关.表明下颌骨骨折患者在一定时期内易出现颞下颌关节紊乱综合征.     

细菌素产生菌的筛选及其细菌素的分离纯化

从香肠中分离得到一株产细菌素的乳酸片球菌，其发酵液经硫酸铵沉淀，CM—Sephadex C50阳离子交换柱层析后，得到的细菌素样品通过SDS—PAGE证明是一条带，分子量约20．83ku，该细菌素对热及酸碱稳定，易被酶降解而失去活性，对许多革兰氏阳性茵有较强的抑制作用，而对革兰氏阴性茵、酵母和霉没有作用。     

精浆蛋白电泳分析的临床意义

目的为了初步了解男性精浆中的蛋白电泳谱以及蛋白谱与男性生育能力的相关关系。方法通过应用SPIFE3000全自动电泳分析仪对20例对照组(生育组)与140例观察组(不育组)的精浆进行蛋白分离(琼脂糖区带电泳法),建立其精浆蛋白质的特征电泳图谱,并进行对比分析。结果精浆蛋白经酸蓝染色后,区带清晰,从阳极到阴极分为A、B、C、D、E 5条带,电泳各区带百分比含量结果经方差分析,发现不育组与生育组A、C、D、E百分比含量变化有统计学意义(P<0.01),且无精子症患者精浆的蛋白谱有特异的"双峰"特点。结论精浆蛋白区带电泳分析可作为不育症的辅助诊断指标,且将是对男性不育诊断手段的有力补充。     

A Bayesian iterative transmission gradient reconstruction algorithm for cardiac SPECT attenuation correction

Background  High-quality attenuation maps are critical for attenuation correction of myocardial perfusion single photon emission computed tomography studies. The filtered backprojection (FBP) approach can introduce errors, especially with low-count transmission data. We present a new method for attenuation map reconstruction and examine its performance in phantom and patient data. Methods and Results  The Bayesian iterative transmission gradient algorithm incorporates a spatially varying gamma prior function that preferentially weights estimated attenuation coefficients toward the soft-tissue value while allowing data-driven solutions for lung and bone regions. The performance with attenuation-corrected technetium 99m sestamibi clinical images was evaluated in phantom studies and in 50 low-likelihood patients grouped by body mass index (BMI). The algorithm converged in 15 iterations in the phantom studies. For the clinical studies, soft-tissue estimates had significantly greater uniformity of mediastinal coefficients (mean SD, 0.005 cm⁻¹ vs 0.011 cm⁻¹; P<.0001). The accuracy and uniformity of the Bayesian iterative transmission gradient algorithm were independent of BMI, whereas both declined at higher BMI values with FBP. Attenuation-corrected perfusion images showed improvement in myocardial wall variability (4.8% to 4.1%, P=.02) for all BMI groups with the new method compared with FBP. Conclusion  This new method for attenuation map reconstruction provides rapidly converging and accurate attenuation maps over a wide spectrum of patient BMI values and significantly improves attenuation-corrected perfusion images.     

X线引导下经皮通用脊柱系统内固定治疗胸腰椎骨折

目的探讨X线引导下经皮通用脊柱系统(USS)内固定治疗胸腰椎骨折的可行性.方法 2002年10月～2004年5月对无须减压胸腰椎骨折8例采用后路经皮USS椎弓根螺钉植入,在椎旁肌深层置入固定棒行伤椎复位固定.结果手术时间120～240 min,平均140 min.术中出血量20～90 ml,平均40 ml.后凸Cobb's角术前(21.5±4.3)°、术后(2.7±1.5)°(t=17.541,P=0.001),椎体塌陷术前18.5%±4.1%、术后2.4%±1.0%(t=16.504,P=0.001).术后3周佩戴腰围支具下床,复查Frankel分级,C级恢复至D级2例,D级恢复至E级1例,保持D级1例,保持E级4例.结论经皮USS内固定治疗无须减压的胸腰椎骨折创伤小,恢复快,但技术难度高,X线暴露时间长.     

生物制品GMP认证检查的几点体会

本文从生物制品的特点、生物制品对厂房设备的特殊要求、GMP验证和人员素质几个方面。阐述了对生物制品实施GMP认证检查的感想和体会。