北海市娱乐场所服务小姐性病艾滋病知识与性病感染情况调查

目的了解北海市娱乐场所服务小姐性病艾滋病知识水平及性病艾滋病感染情况,以便为在公共娱乐场所服务小姐中开展预防性病艾滋病的健康干预工作提供依据.方法对北海市区内娱乐场所采用分层整群抽样方法,随机问卷调查娱乐场所内187名服务小姐的人口学社会学特征、性病艾滋病相关知识,并免费对其进行性病艾滋病检测.结果调查对象年龄平均(24.1±4.9)岁,其中以20～24岁者为主(占47.6%);文化程度以初中为主(占56.1%);来自外省者占多数(45.5%).调查对象对性病艾滋病相关知识回答平均正确率为68.2%,其中对艾滋病主要传播途径认知水平比较高,但对于艾滋病性病的非传播途径回答正确率比较低.在调查对象中未检出HIV感染者,性病总感染率为40.6%.结论北海市娱乐场所服务小姐对性病艾滋病相关知识有一定了解,但不全面;娱乐场所服务小姐是性病高危人群,应采取有针对性的有效措施对娱乐场所服务小组开展干预工作.     

蛋白激酶Cα反义寡核苷酸对人胰腺癌BXPC-3细胞体外侵袭力的影响

目的:观察蛋白激酶Cα(PKCα)反义寡核苷酸(ASODN)对人胰腺癌BXPC-3细胞体外侵袭力的影响.方法:将BXPC-3细胞分为7组:对照组,64 mg/L随机寡核苷酸序列(RODN)转染组,4 mg/L、8 mg/L、16 mg/L、32mg/L和64 mg/L ASODN转染组,RT-PCR方法检测PKCα mRNA表达情况;以有效转染浓度的ASODN转染BXPC-3细胞,并设对照和RODN组,Transwell侵袭小室方法检测BXPC-3细胞体外侵袭能力.结果:16 mg/L、32 mg/L和64 mg/L ASODN转染组PKCα/GADPH mRNA灰度值(0.637 1±0.036 0,0.563 2±0.008 0,0.238 9±0.020 0)均低于对照组(0.912 1±0.025 0)(P＜0.05),RODN组(0.914 7±0.017 0)、4 mg/L ASODN组(0.912 3±0.018 0)和8 mg/L ASODN组(0.911 6±0.029 0)与对照组比较差异均无统计学意义(P＞0.05).与对照组穿膜细胞数(286±10)和RODN组(268±3)比较,转染16 mg/L PKCα ASODN(119±9)可降低胰腺癌BXPC-3细胞的体外侵袭力(P＜0.05).结论:靶向PKCα的ASODN可有效阻断其在人胰腺癌BXPC-3细胞中的表达,并降低癌细胞体外侵袭力.     

正反义人PIN1基因克隆及真核表达载体的构建

目的 克隆正、反义人PIN1基因（hPIN1）及构建hPIN1基因正、反义真核表达载体.方法 应用RT-PCR技术从人骨肉瘤细胞系MG-63细胞总RNA中成功扩增出990bp包含hPIN1基因编码区的cDNA序列,按正、反方向加上BamHⅠ及 HindⅢ双酶切位点后形成正、反义hPIN1基因,然后连入含BamHⅠ及 HindⅢ双酶切位点的pIRES2-EGFP表达质粒上,构建正、反义hPIN1基因的重组真核表达质粒phPIN1.结果 扩增的cDNA经测秩序与基因文库完全一致;BamHⅠ及HindⅢ双酶切后,正、反义表达质粒形成5.3 ＋0.99kb 两条带,与理论计算值完全一致.结论 成功克隆正、反义人PIN1基因及构建hPIN1基因的正、反义真核表达载体.     

灯盏细辛注射液对急性脑梗死患者血清粘附分子sICAM-1和CD11b/CD18的影响

目的 观察灯盏细辛注射液对急性脑梗死(acute cerebral infaction,ACI)患者的临床疗效及对血清粘附分子sICAM-1和CD11b/CD18表达的对影响.方法 69例ACI患者随机分为灯盏细辛治疗组(36例)和常规治疗组(33例),采用双抗体夹心ELISA法、流式细胞仪(FCM)检测两组ACI患者治疗前后血清粘附分子sICAM-1和CD11b/CD18表达的水平.结果 ACI患者治疗前血清粘附分子sICAM-1和CD11b/CD18的表达水平显著高于健康人组(p＜0.05),灯盏细辛治疗组临床有效率明显高于常规治疗组(P＜0.05),两组治疗后血清粘附分子sICAM-1和CD11b/CD18的表达均明显下降,灯盏细辛治疗组降低较常规治疗组更明显(p＜0.05).结论 灯盏细辛注射液治疗ACI可能与其下调血清粘附分子sICAM-1和CD11b/CD18的表达,减轻中枢神经系统的炎性损伤密切相关.     

抗菌药物的非抗菌作用研究

抗菌药物的非抗菌作用有可能成为一种有益的治疗作用,其在临床应用中或许能起到关键作用。     

胰腺结核1例并文献复习

胰腺结核是一种少见的肺外结核,临床症状与影像学表现无特异性,易与其他胰腺疾病混淆,导致抗结核治疗不及时或接受不必要的手术。对于腹腔淋巴结增大、梗阻性黄疸的年轻患者,尤其不伴有肿瘤标志物升高的孤立性胰腺占位,需考虑胰腺结核的可能。完善相关实验室检查,病理组织活检可帮助鉴别诊断,必要时可行手术探查及引流。本文回顾性分析成都公共卫生临床医疗中心收治的1例胰腺结核患者的临床资料、影像学表现,并进行相关文献复习,旨在提高临床对胰腺结核的诊疗水平,减少漏诊、误诊。     

荧光定量PCR仪检测乙型肝炎病毒核酸的性能验证及评价

目的荧光定量聚合酶链反应(PCR)仪检测乙型肝炎病毒核酸(HBV DNA)的性能验证及评价。方法参考ISO15189《医学实验室质量和能力认可准则(2012年)》,以及美国临床和实验室标准化协会(CLSL)EP系列文件的相关要求对实验室HBV DNA荧光定量PCR检测系统的精密度、正确度、可报告范围、抗干扰能力进行验证及评价。结果精密度:HBV DNA定量检测高浓度(106 IU/mL)和低浓度(103 IU/mL)标本的对数值批内变异系数分别为0.67%和3.65%,批间变异系数分别为1.72%和4.52%;正确度:标准物质标本检测结果的对数值与厂商给定靶值浓度的对数值差值在±0.4个Log值内;可报告范围:试验试剂在(2.22×10)～(2.22×108)IU/mL范围内具有良好的线性,线性回归方程为Y=1.027 X-0.408,R2=0.995,≥0.95;抗干扰能力:说明书浓度的干扰物质(血红蛋白、三酰甘油、胆红素)标本结果与不含干扰物质标本比较,其浓度的对数值偏倚CV7.5%,对数值差值在±0.4个Log值内。结论荧光定量PCR仪检测HBV DNA试剂的各项性能特征与厂家声明相符,满足预期用途,适用于临床常规检测。     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Survivin反义寡核苷酸对骨肉瘤细胞凋亡的诱导效应

目的：利用骨肉瘤OS-732细胞，探讨survivin反义寡核苷酸（ASODN）对survivin基因表达的影响及其诱导肿瘤细胞凋亡的效应。 方法: 设计合成特异性靶向survivin的ASODN，以不同浓度和时间对OS-732细胞进行转染，并设空白、正义（SODN）对照组进行比较。采用RT-PCR、Western blotting、免疫细胞化学技术检测各组OS-732细胞中survivin mRNA、蛋白表达状态，吖啶橙/溴化乙锭（AO/EB）染色法、流式细胞仪检测细胞凋亡水平和形态变化，MTT法检测细胞生长抑制情况，激酶活性测定法检测细胞中caspase-3活性程度。 结果: 转染ASODN各组OS-732细胞survivin mRNA和蛋白表达均明显弱于空白对照组、SODN组；细胞凋亡率（AI）显著增加，细胞生长相应受抑，caspase-3活性显著提高；上述效应在ASODN各组存在一定的时间浓度依赖性；而SODN各组与空白对照组间各项指标均无明显差异。 结论: ASODN能够特异性下调OS-732细胞中survivin基因表达，激活凋亡效应蛋白酶caspase-3，在诱导肿瘤细胞凋亡、抑制增殖中发挥重要作用。     

凹槽交锁髓内钉治疗开放性胫骨骨折

目的评价凹槽交锁髓内钉治疗开放性胫骨骨折的疗效.方法应用德国aap公司Birigid非扩髓凹槽交锁髓内钉治疗57例开放性胫骨骨折.髓内钉直径平均7.8mm.结果所有患者得到4～22个月随访.骨折平均愈合时间28周.1例Ⅲa型伤口感染,2例Ⅲ型骨折延迟愈合,经改动力化后获得骨性愈合,无断钉发生,无骨不连.结论Biorigid凹槽交锁髓内钉治疗胫骨开放性骨折,只要严格掌握手术时机,彻底清创,具有操作简单,创伤小,适应症范围广,术后早期活动,骨折愈合率高,感染率低以及不扩髓等优点.