排序方式: 共有48条查询结果,搜索用时 15 毫秒
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肝移植受者术后出现精神心理异常可能是生物-心理-社会等综合因素影响下引起的中枢神经系统功能紊乱,影响肝移植受者生存质量并降低移植成功率。本文综述了肝移植受者并发精神心理异常的原因、症状识别和防治策略,期望有助于肝移植受者并发精神心理异常的早期识别和干预,以改善受者预后。 相似文献
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目的:研究中药复方"松友饮"含药血清对体外培养的人肝癌MHCC97H细胞增殖、侵袭潜能的影响。方法:以MHCC97H细胞构建裸鼠原位移植瘤模型,84只成模裸鼠随机平均分成对照组和"松友饮"组,肝脏接种肿瘤24 h内开始1次/天灌胃给药。在第1、4、7、14、21、28、35次灌胃结束后2h,每组分别处死6只裸鼠收集血样。应用含药血清体外干预MHCC97H细胞,检测细胞增殖、侵袭潜能变化。结果:"松友饮"灌胃给药1、4、7天的荷HCC裸鼠含药血清体外抑制HCC作用逐渐增强,给药7、14、21、28、35天的含药血清抑制作用无统计学差异。灌胃7天的"松友饮"含药血清抑制HCC增殖的作用与中剂量卡培他滨作用近似,抑瘤率分别为37.4%、41.1%。给药7天的含药血清抑制HCC侵袭潜能达到最强,"松友饮"组穿膜细胞数明显少于对照组,为26.0±3.6和37.3±2.8。结论:给药7天的含药血清达到稳态血药浓度,对MHCC97H细胞的体外抑制作用达到最强。 相似文献
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中药复方“松友饮”及其组方药材提取物高效液相指纹图谱研究 总被引:1,自引:0,他引:1
目的:分析中药复方"松友饮"及组方药材提取物指纹图谱用于质量控制。方法:采用高效液相色谱法(high-performance liquid chromatography,HPLC)研究中药指纹图谱。包括低压四元泵,自动进样器和二极管阵列检测器(DAD),Agilent Zorbax RX-C8色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流动相为水-乙腈,进行梯度洗脱(流速,0.5 mL/min;洗脱时间,60 min;检测波长,280nm)。结果:成功构建"松友饮"及组方提取物高效液相指纹图谱,色谱保留时间稳定,各主要色谱峰偏移小于0.1 min。通过比对发现"松友饮"HPLC色谱峰来自组方药材成分。重复HPLC色谱相似度(90%,精密度、稳定性、重复性实验中各共有峰相对保留时间和相对峰面积RSD(3%。结论:HPLC方法可靠、可行,指纹图谱重复性良好,为"松友饮"质控提供参考。 相似文献
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中药"松友饮"对高转移人肝癌侵袭性及小鼠免疫功能的影响 总被引:10,自引:2,他引:10
目的:研究“松友饮”对高转移人肝癌MHCC97H细胞侵袭性和小鼠免疫功能的影响及其机制。方法:用不同质量浓度的“松友饮”(2、4、8mg/mL)分别干预MHCC97H细胞48h,用明胶酶谱实验及侵袭实验研究基质金属蛋白酶-2(matrix metalloproteinase-2。MMP-2)活性和侵袭性变化。用高转移人肝癌裸鼠原位移植瘤模型进行体内实验,以检测对“松友饮”对肿瘤的抑制作用。C57BL/6小鼠和昆明种小鼠均随机分为“松友饮”剂量递增的C1、C2和C3组及阴性、阳性对照组,灌胃给药,每天1次,连续7d,处死小鼠后检测自然杀伤细胞(natural killer cell,NK)的活性,观察小鼠腹腔巨噬细胞的吞噬功能变化。结果:与对照组相比。“松友饮”4、8mg/mL组细胞侵袭性下降有统计学意义(P〈0.01),对应的MMP-2活性下降。此外,“松友饮”抑制了肝癌细胞生长,C1、C2和C3组的NK细胞特异性抑制百分率分别为24.6%、27.1%和27.7%,巨噬细胞吞噬百分率与对照组相比,差异有统计学意义(P〈0.01)。结论:中药复方“松友饮”可能通过抑制MMP-2活性来抑制肝癌侵袭性,连续7d“松友饮”灌胃给药能增强小鼠免疫功能。 相似文献
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目的:分析中药复方"松友饮"体内、外抑癌作用。方法:明确"松友饮"的组方成份,总结前期相关研究结果,提出尚需进一步研究内容。结果:"松友饮"为5味中药提取物,已分析获得高效液相指纹图谱,为"松友饮"提供质控参考。"松友饮"通过抑制MMP2抑制肝癌(HCC)侵袭性;连续7天灌胃给药增强小鼠免疫功能。连续5周灌胃抑制术后残癌组织IL–1β和PCNA表达,抑制裸鼠模型HCC生长、转移,延缓裸鼠体重下降,延长生存期,增强干扰素(抑制HCC。给药7天的含药血清达到稳态血药浓度,对HCC体外抑制作用最强。该复方已开展临床研究,但药效物质基础仍需深入研究。结论:"松友饮"具有体内、外抑制HCC生长、转移作用,具有潜在临床应用价值。 相似文献
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Objective To investigate the effects of Pseudomonas aeruginosa vaccine (PA) on proliferation and invasiveness of the hepatocellular carcinoma cell line MHCC97L with metastatic potential. Methods Proliferation, growth curve, plate efficiency, flow cytometry, transwell invasion assay, cell motility assay, scarification test, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP2) protein activity were evaluated after cells were treated with PA at various concentrations. Results PA can inhibit the proliferation and plate efficiency of MHCC97L cell markedly in a dose-dependent manner. The IC50 of cells treated with PA for 48 h and 72 h was 3.1 ×108/ml and 1.9 × 108/ml, respectively. The doubling time increased and plate efficiency decreased gradually when cells treated with 0.5 × 108/ml, 1 × 108/ml and 2 × 108/ml PA (P<0.01). PA could induce cell cycle arrest at the G1 phase in a dose-dependent manner by flow cytometric analysis. The average amount of invading cell per field in cell invasion assay and motility assay were 4. 8 ± 1.3 and 8. 8±2.2 when cells treated with 1× 108/ml PA, which was significantly lower than that of control group (8. 6±2. 1 and 15. 6±1.2 ) (P<0.01) Scarification test showed that the metastatic ability of cells treated with 1 × 108/ml PA significantly lower than that in the control group. Comparison between cells treated with 1 × 108/ml PA and control group, no remarkable difference was found regarding expression of VEGF and MMP2 in supernatant of cell culture. Conclusion PA can inhibit proliferation and plate efficiency of HCC cell line MHCC97L, which is in part mediated by the cell cycle arrest at the G1 phase. PA could inhibit invasiveness of HCC cell line MHCC97L, which is unrelated to the VEGF and MMP2 protein activity. 相似文献
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