吴茱萸和制吴茱萸的饮片、水煎液、配方颗粒UPLC特征图谱研究

目的:建立吴茱萸和制吴茱萸饮片、水煎液、配方颗粒的超高效液相色谱(UPLC)特征图谱,比较其相关性和差异性。方法:采用UPLC法。色谱柱为YMC Triart C₁₈,流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),流速为0.3 mL/min,检测波长为254nm,柱温为30℃,进样量为1μL。以柠檬苦素为参照,绘制吴茱萸和制吴茱萸饮片、水煎液、配方颗粒(各10批,共60批)的特征图谱,利用《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价,确定共有特征峰;采用方差分析评价共有特征峰峰面积占比的差异,并采用SPSS 20.0软件对吴茱萸和制吴茱萸的饮片、水煎液、配方颗粒进行聚类分析和主成分分析。结果:吴茱萸样品和制吴茱萸样品(饮片、水煎液和配方颗粒)的特征图谱中分别均含有共有特征峰16、17个,指认8、9、11、17号峰分别为柠檬苦素、吴茱萸碱、吴茱萸次碱和甘草酸;与饮片比较,水煎液和配方颗粒的特征峰相似度均低于0.55,而水煎液与配方颗粒特征峰的相似度均高于0.95。聚类分析和主成分分析结果显示,吴茱萸饮片和制吴茱萸饮片聚为一类,吴茱萸水煎液、配方颗粒...     

泪腺脱垂的手术治疗体会

随着人们生活水平的日益提高 ,对美的追求与日俱增 ,尤其是对眼部整形 ,作者在 1995— 0 1～ 2 0 0 0— 10用切开法、压线法、埋线法行重睑术 10 8例 (包括老年性睑内翻 ) ,发现泪腺脱垂者 6例 ,并行泪腺切除复位手术 ,手术效果满意。1　对象和手术方法1.1　作者在门诊 6年中收治 10 8例 ,男 9例 ,女 99例 ,年龄18～ 68岁 ,平均 3 6.3岁 ,发现泪腺脱垂者 6例 ,7只眼 ,1例双眼 ,5例为单眼 ,均为女性。年龄 2 6～ 4 5岁 ,平均 3 7.1岁 ,5例病人跟踪半年 ,1例未就诊。1.2　手术方法术前设计好切口线 ,有眼睑松弛者再划好切皮线 ,剪开皮肤 ,有的…     

腹腔化疗治疗癌性腹水临床观察

目的探讨并观察腹腔内化疗治疗腹腔内恶性肿瘤引起的癌性腹水的疗效及毒副反应.方法 对30例腹腔内恶性肿瘤患者经B超及CT诊断为中等至大量腹水,根据肿瘤疾病不同予以腹腔内注入顺铂(DDP)20~40mg,5Fu 500mg,羟基喜树碱(HCPT)4~8mg加生理盐水200ml,一周后复查血常规正常,可重复给药一次,连续两次,休息一月后复查.同时观察疗效及毒副反应.予对症处理.结果 治疗有效率(CR+PR)80%,毒副作用轻,患者自觉症状缓解.生活质量提高.结论 腹腔化疗治疗腹内恶性肿瘤所致癌性腹水.疗效明显.毒副作用轻,是一种有效的姑息治疗方法.     

五柱绞股蓝总皂苷和总黄酮的季节性变化

目的 测定五柱绞股蓝茎和叶中的总皂苷和总黄酮的量，并揭示其在不同季节内的动态变化规律。方法 以绞股蓝皂苷和芦丁为对照品，采用紫外可见光分光光度计在 550 nm 处测定皂苷吸光度，在 510 nm 处测定黄酮吸光度。结果 五柱绞股蓝茎、叶中的总皂苷和总黄酮的量在5月至11月的生长发育季节内，均表现为先上升后下降的变化规律，以9月的量最高，且叶中的量高于茎中的量。结论 五柱绞股蓝采收可安排在8～9月的花果期进行。     

塞来昔布对SK-N-SH细胞增殖和凋亡影响的研究

目的:探讨特异性环氧化酶-2(COX-2)抑制剂塞来昔布(celecoxib)对人神经母细胞瘤细胞系SK-N-SH细胞生长的影响及其分子生物学作用机制。方法:不同浓度塞来昔布(12.5、25、50和75μmol/L)用不同时间(24、48和72h)处理SK-N-SH细胞,MTT法检测细胞增殖,DNA ladder法及AO/EB染色法分析细胞凋亡,Western blot检测COX-2、Bcl-2蛋白表达。结果:MTT法显示,12.5、25、50和75μmol/L组在3个时间点对细胞的抑制率分别为(7.38±1.12)%、(10.33±1.97)%和(25.16±5.58)%;(34.46±6.76)%、(30.12±6.71)%和(57.54±3.06)%;(61.85±4.01)%、(50.78±2.85)%和(85.67±2.17)%;(83.85±5.56)%、(90.06±5.71)%和(98.04±4.43)%。组间差异均有统计学意义,P<0.05。结论:塞来昔布抑制SK-N-SH细胞的生长并诱导其凋亡,其机制除了抑制COX-2外还可能与抑制Bcl-2有关,有一定的临床应用价值。     

43例淋巴母细胞淋巴瘤患者临床特点及疗效分析

Objective To investigate the clinical features and treatment outcomes of different regimens in Chinese patients with lymphoblastic lymphoma(LBL). Methods Forty-three patients with LBL were retrospectively analysed, of which 30 were T-LBL, and 13 B-LBL. Results ①Most patients were young men with a median age of 21, and 63.0％ of the T-LBL patients had mediastinal masses. ② Treatment outcome could be assessed in 37 cases, of which the response rate (RR) was 81.1％ and complete remission (CR) rate was 67.6％. The RR and CR rates in patients treated with regimens for ALL (ALL-like group) and those treated with regimens for NHL(NHL-like group) were 94.4％ , 68.4％ and 83.3％ , 52.6％ , respectively. ③The estimated median overal survival(OS) and progression free survival (PFS) of hematopoietic stem cell transplantation (HSCT) group were significant longer than those of ALL-like group(P =0.018, P=0.025) and NHL-like group(P = 0. 016, P = 0. 011). The OS at 5 years in NHL-like group, ALL-like group and HSCT group were (14.4 ± 9.4) ％ , (20.2 ± 12.7) ％ and (79.5 ± 13.1) ％, respectively. Conclusion ①LBL is more common in young men, with less involvement of peripheral blood. Compared with B-LBL, T-LBL often has a mediastinal mass and serious cavity effusion. ② Intensive treatment regimens for ALL should be used in LBL. HSCT at CR1 can improve outcome obviously.     

43例淋巴母细胞淋巴瘤患者临床特点及疗效分析

Objective To investigate the clinical features and treatment outcomes of different regimens in Chinese patients with lymphoblastic lymphoma(LBL). Methods Forty-three patients with LBL were retrospectively analysed, of which 30 were T-LBL, and 13 B-LBL. Results ①Most patients were young men with a median age of 21, and 63.0％ of the T-LBL patients had mediastinal masses. ② Treatment outcome could be assessed in 37 cases, of which the response rate (RR) was 81.1％ and complete remission (CR) rate was 67.6％. The RR and CR rates in patients treated with regimens for ALL (ALL-like group) and those treated with regimens for NHL(NHL-like group) were 94.4％ , 68.4％ and 83.3％ , 52.6％ , respectively. ③The estimated median overal survival(OS) and progression free survival (PFS) of hematopoietic stem cell transplantation (HSCT) group were significant longer than those of ALL-like group(P =0.018, P=0.025) and NHL-like group(P = 0. 016, P = 0. 011). The OS at 5 years in NHL-like group, ALL-like group and HSCT group were (14.4 ± 9.4) ％ , (20.2 ± 12.7) ％ and (79.5 ± 13.1) ％, respectively. Conclusion ①LBL is more common in young men, with less involvement of peripheral blood. Compared with B-LBL, T-LBL often has a mediastinal mass and serious cavity effusion. ② Intensive treatment regimens for ALL should be used in LBL. HSCT at CR1 can improve outcome obviously.     

五柱绞股蓝总皂苷和总黄酮的季节性变化

目的 测定五柱绞股蓝茎和叶中的总皂苷和总黄酮的量,并揭示其在不同季节内的动态变化规律.方法 以绞股蓝皂苷和芦丁为对照品,采用紫外可见光分光光度计在550 nm处测定皂苷吸光度,在510 nm处测定黄酮吸光度.结果 五柱绞股蓝茎、叶中的总皂苷和总黄酮的量在5月至11月的生长发育季节内,均表现为先上升后下降的变化规律,以9月的量最高,且叶中的量高于茎中的量.结论 五柱绞股蓝采收可安排在8～9月的花果期进行.     

奈达铂治疗恶性肿瘤研究进展

铂类化合物是治疗实体肿瘤的主要药物,自从1967年人们发现顺铂(cisplatin,DDP)有抗肿瘤活性以来,铂类金属抗肿瘤药的应用和研究得到了迅速的发展。奈达铂(nedaplatin,NDP;顺甘醇酸二氨合铂)是日本盐野义制药公司开发的第2代铂类抗肿瘤药,1995年在日本首次获准上市,目前,NDP主要用于头颈部肿瘤、肺癌、子宫癌、食道癌、膀胱癌、睾丸癌等,     

清开灵注射液的不良反应

报道清开灵注射液致变态反应、急性喉阻塞、洋地黄毒性反应、急性左心衰竭等不良反应。