135G>C and 172G>T polymorphism in the 5' untranslated region of RAD51 and sporadic endometrial cancer risk in Polish women

BACKGROUND : Despite advanced diagnostic and therapeutic procedures, endometrial cancer (EC) is still responsible for high morbidity and mortality of women. The genetic variability in RAD51 may contribute to the appearance and progression of various cancers including EC. AIM : We investigated the association of polymorphisms in the DNA repair genes RAD51 135G>C and 172G>T with endometrial cancer risk. MATERIAL AND METHODS : The genotypes of RAD51 135G>C and 172G>T polymorphism were determined by PCR-RFLP methods in endometrial tissue of 240 cancer subjects and 240 healthy subjects who served as controls. RESULTS : In the present work we demonstrated a significant positive association between the RAD51 C/C genotype and endometrial carcinoma, with an adjusted odds ratio (OR) of 13.0 (p < 0.0001). The distribution of genotypes for 135G>C SNP in endometrial cancer patients vs. controls was: 10% vs. 27% for GG, 13% vs. 58% for GC and 77% vs. 15% for CC genotype, respectively. Variant 135C allele of RAD51 increased the cancer risk (OR = 1.81; 95% CI 0.11-2.93, p = 0.022). The higher risk of EC occurrence was associated with the combined C135C-G172T genotype (OR = 7.69; 95% CI 3.45-17.12). CONCLUSION : The results indicated that the polymorphism 135G>C of the RAD51 gene may be positively associated with endometrial carcinoma in the Polish population. Further studies, conducted on a larger group, are required to clarify this point.     

Borelli's lactritmel agar induces conidiation in rare-macroconidia producing dermatophytic fungi

Macroconidia are among the most important indicators used to identify dermatophytic fungi, but several do not usually sporulate and/or produce macroconidia on Sabouraud glucose agar. Specifically, Microsporum audouinii, M. ferrugineum, Trichophyton concentricum, T. schoenleinii, T. verrucosum, and T. violaceum (including T. soudanense and T. yaoundei) rarely form macroconidia and, therefore, cannot be easily identified. In this study, we investigated the production of macroconidia on nine common laboratory media, including Borelli's lactritmel agar (BLA), modified Borelli's lactritmel agar (MBLA), brain heart infusion agar (BHIA), Christensen's urease agar in Petri dishes (UPA), cornmeal dextrose agar (CMDA), Lowenstein-Jensen agar (LJA), malt extract agar (MEA), oatmeal agar (OA), and potato dextrose agar (PDA). The performance of these media was evaluated using 18 rare-macroconidia producing isolates, including representative of the six species mentioned above. All cultures in this study were incubated at 26°C on the bench, and conidia formation on each was investigated at 5, 10, 15, 20, 25, and 30 days of incubation. BLA apparently improved macroconidia production after 15 days and was the most useful nutrient agar medium to induce these phenotypic characters in daily practice, closely followed by OA, PDA, and MBLA.     

术中唤醒麻醉下语言区肿瘤切除术的临床研究

目的探讨全身麻醉术中唤醒方法行语言区肿瘤切除术的安全性和可行性。方法选择位于大脑语言区肿瘤患者9例,麻醉前一天用焦虑自评量表(SAS)测定患者焦虑状况。对患者进行喉罩通气,罗哌卡因头皮浸润麻醉,丙泊酚和瑞芬太尼靶控输注,间断静脉推注舒芬太尼。术中唤醒后行语言功能监测(指认图片、连续计数),并行唤醒后的警觉/镇静评分(OAA/S)、视觉模拟评分(VAS),再行神经电生理刺激,确定语言区和切除范围。术后随访3个月。结果 8例患者麻醉前SAS评分为(45.6±1.7)分,唤醒后OAA/S为(4.8±0.2)分,VAS为(0.7±0.3)分,镇痛完善,完全配合术者要求进行语言功能监测和神经电生理定位语言区。1例患者麻醉前SAS评分为62分,唤醒后OAA/S为2.5分,VAS为8.0分,因唤醒后术中出现癫痫持续发作,放弃唤醒。随访3个月,8例患者功能完全恢复正常,1例存在轻度语言功能障碍。结论在唤醒麻醉下行语言功能监测及神经电生理定位有助于最大程度地切除语言区病灶,提高患者术后生存质量。     

术中唤醒麻醉下语言区肿瘤切除术的临床研究

目的探讨全身麻醉术中唤醒方法行语言区肿瘤切除术的安全性和可行性。方法选择位于大脑语言区肿瘤患者9例,麻醉前一天用焦虑自评量表（SAS）测定患者焦虑状况。对患者进行喉罩通气,罗哌卡因头皮浸润麻醉,丙泊酚和瑞芬太尼靶控输注,间断静脉推注舒芬太尼。术中唤醒后行语言功能监测（指认图片、连续计数）,并行唤醒后的警觉／镇静评分（OAA／S）、视觉模拟评分（VAS）,再行神经电生理刺激,确定语言区和切除范围。术后随访3个月。结果8例患者麻醉前SAS评分为（45．6±1．7）分,唤醒后OAA／S为（4．8±0．2）分,VAS为（0．7±0．3）分,镇痛完善,完全配合术者要求进行语言功能监测和神经电生理定位语言区。1例患者麻醉前SAS评分为62分,唤醒后OAA／S为2．5分,VAS为8．0分,因唤醒后术中出现癫痫持续发作,放弃唤醒。随访3个月,8例患者功能完全恢复正常,l例存在轻度语言功能障碍。结论在唤醒麻醉下行语言功能监测及神经电生理定位有助于最大程度地切除语言区病灶,提高患者术后生存质量。     

舒芬太尼对老年患者全麻诱导期间血流动力学的影响

目的观察不同剂量舒芬太尼和芬太尼复合丙泊酚在老年患者全麻诱导期对血流动力学的影响,探讨舒芬太尼在老年患者全麻诱导的合适剂量。方法 ASA分级Ⅰ-Ⅱ择期手术患者60例,年龄65-75岁,分Ⅰ组（芬太尼组,n=15）、Ⅱ组（舒芬太尼,0.2μg/kg,n=15）、Ⅲ组（舒芬太尼0.4μg/kg,n=15）、Ⅳ组（舒芬太尼0.6μg/kg,n=15）,四组患者全麻诱导时均给予：咪达唑仑0.05mg/kg,丙泊酚0.8mg/kg,顺式苯磺酸阿曲库铵0.3mg/kg。分别记录四组患者的在诱导前T0,插管前T1,插管1min T2、3min T3、5min T4,记录血压、心率变化。结果与T0相比,Ⅳ组BP在各时相均低于基础值,且与Ⅲ组差异有统计学意义（P〈0.05）;插管后Ⅰ组和Ⅱ组BP在T2、T3、T4均超过基础值且与其他两组差异有统计学意义（P〈0.05）。结论舒芬太尼0.4μg/kg在全麻插管期间有较为稳定的血流动力学。     

远端肢体缺血预处理对氯胺酮致发育期大鼠海马CA1区神经细胞Bcl-2和Bax表达失衡的影响

目的研究远端肢体缺血预处理对氯胺酮麻醉导致的发育期大鼠海马CA1区神经细胞抗凋亡因子B淋巴细胞瘤-2(Bcl-2)和凋亡因子Bcl-2相关X蛋白(Bax)表达失衡的影响。方法SD新生大鼠48只,5日龄,体重8~12 g,随机分为四组:对照组(C组)于出生第7天腹腔注射生理盐水8 ml/kg,间隔2 h注射一次,共注射6次;远端肢体缺血预处理组(RIPC组)于出生第5天行右后肢缺血5 min,再灌注5 min预处理,4个循环,48 h后腹腔注射生理盐水8 ml/kg,方法同C组;氯胺酮组(K组)于出生第7天腹腔注射氯胺酮20mg/kg,方法同C组;肢体缺血预处理+氯胺酮组(RK组)于出生第5天行远端肢体缺血预处理,48 h后腹腔注射氯胺酮20 mg/kg,方法同C组。四组于腹腔注射完成后12 h经心脏灌注取脑,分别采用免疫组化法和Western blot法观察大鼠海马CA1区凋亡因子Bax和抗凋亡因子Bcl-2阳性细胞数量和蛋白含量。结果与RIPC组比较,K组和RK组海马CA1区神经细胞Bax阳性细胞数量明显增多,蛋白含量明显升高(P<0.05),Bcl-2阳性细胞数量明显减少,蛋白含量明显降低(P<0.05)。C组和RIPC组海马CA1区神经细胞Bcl-2和Bax阳性细胞数量和蛋白含量差异无统计学意义。与K组比较,RK组海马CA1区神经细胞Bax阳性细胞数量明显减少,蛋白含量明显降低(P<0.05),Bcl-2阳性细胞数量明显增多,蛋白含量明显升高(P<0.05)。结论远端肢体缺血预处理可减轻氯胺酮大剂量、多次注射导致的发育期大鼠海马CA1区神经细胞Bcl-2、Bax表达失衡。     

On the role of RNA binding proteins in polyglutamine diseases:from pathogenesis to therapeutics

<正>Polyglutamine(polyQ) diseases are a group of different neurodegenerative disorders characterized by an abnormal expansion of the trinucleotide cytosine-adenine-guanine(CAG)within coding regions of each disease-associated gene.The abnormal expansion translates into a protein bearing an abnormally long tract of glutamines.     

不同剂量右美托咪定对脑外科手术全身麻醉患者术后认知功能及TGF-β/Smad通路的影响

目的 探讨不同剂量右美托咪定对脑外科手术全身麻醉患者术后认知功能及转化生长因子-β(TGF-β)/Smad通路的影响。方法 选取2020年1月—2023年1月宁夏医科大学总医院收治的114例行血肿清除术患者为研究对象,分为A、B、C组,每组38例,分别以0.2、0.4、0.8μg/(kg·h)泵注右美托咪定。比较3组患者围手术期指标,血流动力学,镇痛、镇静及认知功能评分,TGF-β₁和Smad2水平,以及不良反应发生情况。结果 C组丙泊酚用量低于A、B组,B组丙泊酚用量低于A组(P<0.05)。给药后10 min(T₁),C组血氧饱和度(SpO₂)低于A、B组,B、C组收缩压(SBP)低于A组(P<0.05);拔管时(T₂),B、C组心率(HR)、SBP低于A组,C组SpO₂低于A、B组(P<0.05)。拔管后15 min(T₃),B、C组视觉模拟评分(VAS)低于A组,Ramsay评分高于A组(P<0.05);拔管后30 min(...