~（51）Cr标记癌细胞检测癌转移灶

~（５１）Ｃｒ标记癌细胞检测癌转移灶朱启建，张琦，朱金昌，夏玉祥，陈国荣，邵黎明，胡芸用５１Ｃｒ标记癌细胞，观察其在动物体内的转移过程，以探索此法的意义及５１Ｃｒ在体内的衰变规律。一、材料与方法昆明种杂交系小鼠５７只，体重１８～２２９，鼠Ｕ１４癌细胞...     

完全性房室通道伴Ⅱ型动脉总干一例尸检

完全性房室通道伴Ⅱ型动脉总干一例尸检陈国荣邵黎明胡芸患儿男，３天。因吸奶费力３天，气促发绀１天而于１９９５年１０月３１日入院。体检，呼吸６０次／分，心率１６０次／分，胸骨左缘第２、３肋间可闻及Ⅲ～Ⅳ级收缩期杂音伴震颤，阴茎短小。经抢救无效而死亡。患儿...     

可控性回结肠膀胱术11例报告

为了改善膀胱癌根治术后患者的生活质量,我院于1992年2月～1998年10月施行可控性回结肠膀胱术11例,其中IndianaPouch术式6例,去结肠可控回肠膀胱术5例,效果良好,报告如下。1资料和方法1·1一般资料本组11例,男10例,女1例,年龄42～72岁。10例为复发性膀胱移行上皮癌,均有1～4次开放性手术或经尿道肿瘤电切除术。1例为鳞癌。11例均符合膀胱全切除揩征。IndianaPouch本式6例,平均手术时间6．sh,术中平均输血800ml。5倒去结肠带可控性回结肠膀胱术,手术平均时间5．5h,术中平均输血600ml。1·2手术方法根治性膀胱全切除术按常规进…     

岩舒注射液治疗晚期癌症临床观察

岩舒注射液又名复方苦参注射液,是山西金晶药业公司研制的纯中药抗癌新药。我院肿瘤科自1996年5月～1997年6月应用此药治疗晚期癌症72例,并以同期51例晚期癌症作为对照,现报道如下。1 资料与方法1.1 病例选择 全部为住院治疗患者,除原发性肝癌为首次治疗外,均为化疗、放疗、手术后复发的晚期癌症患者,临床分期为Ⅱ～Ⅲ期。分治疗组与对照组,治疗组男43例,女29例;年龄40岁～70岁;肺癌16例,食管癌30例,其中食管癌并锁骨上淋巴结转移15例,根治术后并纵膈淋巴结转移11例,放     

198例肺不张患者的支纤镜检查分析

目的探讨纤维支气管镜检查在肺不张病因诊断中的价值。方法选取我院2005年7月至2009年9月收支的198例肺不张患者的资料，所有患者均进行纤维支气管镜检查，观察纤维支气管镜诊断的准确率，并总结引起肺不张患者的病因。结果198例肺不张患者有192例患者诊断准确，诊断准确率为96．97％。引起肺不张最常见的病因为肺癌，有128例，占64．65％，以鳞癌最为多见。其余依次为肺炎50例，占25．25％，结核16例，占8．08％，其他呼吸系统疾病4例，占2．02％。结论纤维支气管镜是确定肺不张病因的最重要的检查手段之一，可以指导肺不张的临床用药，对于原因不明的肺不张应首选纤维支气管镜检查。临床诊断正确率较高，值得在临床推广。     

原子荧光测汞仪快速测定尿中汞的方法研究

本法采用溴化的方法制备样品．用PSA10025型原子荧光自动测汞仪Millennium Medin系统进行分析检测尿巾汞（Hg）含量，Hg的检出限达0．1ng／L，结果表明，本法能完全满足检测要求．并且是一个快速、灵敏和准确的测定尿液中低水平汞的方法。     

改良院内心肺复苏方法对脑复苏的影响

心肺复苏(CPR)的成功率近年虽然已有了明显提高,但是脑复苏是CPR最后成败的关键.本研究对发生在医院内CPR成功患者的相关资料进行回顾性分析,以探讨提高脑复苏成功率的方法.     

The effects of gossypol on expressions of transforming growth factor-β1, fibronectin and 11β-hydroxysteroid dehydrogenase in nephridial tissue in rats with diabetes mellitus

Objective To observe the effects of gossypol on expressions of transforming growth factor-β1 (TGF-β1), fibronectin (FN) and 11β-hydroxysteroid dehydrogenase (11β-HSD) in nephridial tissue in rats with diabetes mellitus. Methods Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control, type 2 diabetes and gossypol treatment group . After high-fat feeding for 4 weeks, the later two groups were injected with low dosage strepozotocin (30 mg/kg) intraperitoneally to induce type 2 diabetic rat model. The rats in gossypol treated group were given gossypol at the dosage of 15 mg/kg once per day for 4 weeks by gavage. And since the 5th week, the times of gavages had been changed into once per week at the same dosage and lasted to the 12th week . The levels of blood glucose, total cholesterol (TC), low density lipoprotein-cholesterol (LDL-c) were measured. Additionally, the morphological changes of the kidney were studied by light microscopy and transmission electron microscopy respectively. The mRNA expressions of TGF-β1, FN, 11β-HSD and 11β-HSD2 in nephridial tissue were assayed by semi-quantity RT-PCR. The protein expressions of TGF-β1 and FN in nephridial tissue were determined by immunohistochemistry. Results The blood levels of glucose, TC and LDL- c were increased significantly in type 2 diabetic group compared with normal control group(P<0. 01). The volume of glomerulus and the deposition of PAS positive substance in the glomerular interstitium were increased under light microscopy, and the glomerular basal membrane was thicker in type 2 diabetic group than those in normal control group under transmission electron microscopy. The mRNA and protein expressions of TGF-β1 and FN were increased(P<0. 01), and the mRNA expression of 11β-HSD2 was decreased(P<0. 05), while the mRNA expression of 11β-HSD1 was unchanged in type 2 diabetic group compared with normal control group. After the treatment of gossypol, the level of the blood glucose was significantly decreased(P< 0. 01), and the levels of TC, LDL-c showed a trend of decrease but had no statistical differences compared with type 2 diabetic group. The morphology of nephridial tissue was ameliorated in gossypol treatment group. The mRNA and protein expressions of TGF-β1 and FN were decreased(0. 16± 0. 02,0. 22±0. 05 ; 0. 24±0. 06,0. 33±0. 07, P< 0. 05), while the mRNA expressions of 11β-HSD1 and 11β-HSD2 were unchanged compared with type 2 diabetic group. Conclusions Gossypol can relieve the pathologic changes of nephridial tissue, inhibit the expressions of TGF-β1 and FN through decreasing blood glucose of rats with diabetes mellitus.     

银杏叶提取物对糖尿病大鼠心肌内皮素和一氧化氮水平的影响

内皮素（ET）和一氧化氮（NO）与糖尿病慢性并发症的发生、发展密切相关。我们以往的研究表明银杏叶提取物（extract of Ginsko biloba，EGb）可通过抗脂质过氧化作用和降低NO水平而对糖尿病心肌产生保护作用。为进一步探讨其作用机制，本文观察了EGb对链脲佐菌素诱导的糖尿病大鼠血浆、心肌组织NO和ET水平的影响。     

13例手部低毒感染患者的观察与护理

手部慢性低毒感染的病因之一,是由除结核分枝杆菌和麻风分枝杆菌以外的非结核分枝杆菌(nontuberculous mycobacteria,NTM)感染引起。其临床表现为早期在手的受伤部位出现无痛性肿胀。晚期形成手部局限性迁延不愈的溃疡创面,表现呈多样性,可出现弥漫性肉芽肿,炎症性硬结,皮肤慢性溃疡,慢性增生性滑膜炎,反复发作的化脓性关节炎和受累肌腱坏死等。一般较少发生侵袭骨质及肢体的大范围溃