双克氏针内固定治疗106例锁骨中1／3骨折

用双克氏针髓内固定治疗锁骨骨折简单、实用、经济。我院自2000年10月～2004年10月收治锁骨骨折198例，其中106例采用Chaithavat提出的双根克氏针逆行平行固定针尾折弯置于锁骨胸骨端治疗锁骨中1／3骨折疗效显著，现报告如下。     

小剂量炔雌醇治疗绝经后妇女冠心病心绞痛50例

绝经期后妇女冠心病的发病率和病死率较绝经期前明显上升,近年来认为与内源性雌激素减少有关。我科从1992年6月始在常规治疗的基础上口服小剂量炔雌醇和安宫黄体酮治疗绝经后妇女冠心病心绞痛50例取得良效,现报道如下。     

The expression of β-adrenoceptors in human pancreatic cancer cell lines and their roles in cell invasiveness

Objective To investigate the effect and mechanism of β-adrenoceptors on norepi-nephrine-induced invasiveness of pancreatic cancer cell lines. Methods The expression of β-adrenocep-tors mRNA in human pancreatic cancer cell lines MiaPaCa-2 and BxPC3 was detected by using RT-PCR. The cells were randomly divided into control group.10 mol/L NE intervention group, 1 mol/L propranolol intervention group and NE + propranolol intervention group. After 48 h , transwell invasiveness test was used to examine the changes in invasive ability of MiaPaCa-2. The expression of MMP-2, MMP-9 and VEGF mRNA was measured by semi-quantitative RT-PCR. The levels of MMP-2 , MMP-9 and VECF proteins were assayed by immunocytochemistry. Results Both MiaPaCa-2 and BxPC3 expressed β1-and β2-adrenocep-tors. The absorbance ( A) values of invasive cells in NE, NE + propranolol, propranolol and control groups were 0.78±0.02 ,0.32±0.03 ,0.26±0.01 and 0.28±0.02 , respectively, and those in NE intervention group were significantly higher than in control and NE + propranolol groups ( P <0.05) . There was no sig-nificant difference in the number of invasive cells between propranolol and control groups ( P > 0. 05) . In NE group , the expression index of MMP-2 , MMP-9 and VEGF mRNA was 0. 87±0.02 , 1.04±0.02 and 0. 92±0. 01 , and the gray value of the protein expression was 131.20±2.34,105.32±7.21 and 115.60 ±5. 03 , respectively, which were higher than those in control and NE + propranolol groups ( P<0.05). There was no significant difference in the expression levels of MMP-2 , MMP-9 and VEGF mRNA and pro-tein between propranolol and control groups ( P>0.05 ) . Conclusion β-adrenoceptors play an important role in the process of norepinephrine-induced invasiveness of pancreatic cancer cells. NE can promote the invasiveness of MiaPaCa-2 through up-regulating the expression of MMP-2 , MMP-9 and VEGF via β-adre-noceptors.     

食管癌及癌前病变中p53、CD44v6蛋白的表达及其意义

目的 定位分析p53、CD44v6蛋白在食管正常黏膜、单纯增生、不典型增生、原位癌及浸润癌中的表达及与食管癌病理特征的关系。方法 采用免疫组化方法检测p53蛋白、CD44v6蛋白在45例原发性食管鳞癌大体标本中的不同病变区域的表达。结果 p63蛋白在食管癌自然病史中呈现渐进性高表达，而CD44v6蛋白则在其中呈现渐进性低表达。p53蛋白表达在单纯增生和不典型增生中有显著性差异(P＜0．01)，CD44v6蛋白则在原位癌与浸润癌中存在显著性差异（P＜0．01)。p53蛋白在食管癌自然病史中的渐进性高表达与CD44v6蛋白在其中的渐进性低表达有相关性(P＜0．01)。结论p53蛋白表达和CD44v6蛋白表达的改变与食管癌自然病史密切相关，可以作为判断食管癌生物学行为的指标。     

The expression of β-adrenoceptors in human pancreatic cancer cell lines and their roles in cell invasiveness

Objective To investigate the effect and mechanism of β-adrenoceptors on norepi-nephrine-induced invasiveness of pancreatic cancer cell lines. Methods The expression of β-adrenocep-tors mRNA in human pancreatic cancer cell lines MiaPaCa-2 and BxPC3 was detected by using RT-PCR. The cells were randomly divided into control group.10 mol/L NE intervention group, 1 mol/L propranolol intervention group and NE + propranolol intervention group. After 48 h , transwell invasiveness test was used to examine the changes in invasive ability of MiaPaCa-2. The expression of MMP-2, MMP-9 and VEGF mRNA was measured by semi-quantitative RT-PCR. The levels of MMP-2 , MMP-9 and VECF proteins were assayed by immunocytochemistry. Results Both MiaPaCa-2 and BxPC3 expressed β1-and β2-adrenocep-tors. The absorbance ( A) values of invasive cells in NE, NE + propranolol, propranolol and control groups were 0.78±0.02 ,0.32±0.03 ,0.26±0.01 and 0.28±0.02 , respectively, and those in NE intervention group were significantly higher than in control and NE + propranolol groups ( P <0.05) . There was no sig-nificant difference in the number of invasive cells between propranolol and control groups ( P > 0. 05) . In NE group , the expression index of MMP-2 , MMP-9 and VEGF mRNA was 0. 87±0.02 , 1.04±0.02 and 0. 92±0. 01 , and the gray value of the protein expression was 131.20±2.34,105.32±7.21 and 115.60 ±5. 03 , respectively, which were higher than those in control and NE + propranolol groups ( P<0.05). There was no significant difference in the expression levels of MMP-2 , MMP-9 and VEGF mRNA and pro-tein between propranolol and control groups ( P>0.05 ) . Conclusion β-adrenoceptors play an important role in the process of norepinephrine-induced invasiveness of pancreatic cancer cells. NE can promote the invasiveness of MiaPaCa-2 through up-regulating the expression of MMP-2 , MMP-9 and VEGF via β-adre-noceptors.     

肺癌骨转移的治疗进展

骨转移是肺癌主要并发症之一,早期一般无明显不适症状,晚期主要表现为局部持续性进行性刺痛和明显的压痛、病理性骨折、椎体转移的脊髓压迫、高血钙及一般状况下降等.肺癌骨转移的发病率现有的报道差异较大,国内报道为30％～40％[1],国外报道为22％～64％[2],但其已经越来越受到临床医生的关注.骨转移是肺癌患者晚期表现之一,已失去手术治疗的指征,是不可治愈的,预后极差,也是导致肺癌患者死亡的一个重要原因.因此,延长患者生存期,减轻患者痛苦,提高患者生活质量,成为肺癌骨转移治疗的主要目标.     

抑郁症辨证论治述要

对古今医家对抑郁症病因病机及其治疗的论述进行分析,认为抑郁症的主要病机是情志内伤、损及脏腑,治疗多以疏肝理气为主,兼以益气养血安神、化痰祛湿、活血化瘀,并重视心理治疗。     

腰椎退行性滑脱症的治疗探讨

目的探讨腰椎管减压、SP内固定器及后外侧植骨融合治疗不稳定型腰椎退行性滑脱症的临床疗效。方法对21例腰椎退行性滑脱症患者行后方入路腰椎管减压、SP内固定器同定、后外侧植骨融合。比较手术前后临床症状改善和X线片滑脱复位程度进行疗效评定。结果21例患者临床症状均明显改善。16例Ⅰ度滑脱术后X线片检查证实完全复位;5例Ⅱ度滑脱,4例完全复位,1例部分复位。手术优良率85．7％。术后无发生神经系统并发症及感染。结论SP内蚓定器治疗腰椎退行性滑脱症复位满意,疗效良好。     

布鲁杆菌病76例疗效分析

目的 探讨急性和亚急性期布鲁杆菌病不同方法治疗的临床效果.方法 将76例急性和亚急性期布鲁杆菌病患者按数字表法随机分为观察组(40例)和对照组(36例).观察组给予利福喷汀、多西环素和头孢他啶;对照组给予利福平、多西环素,疗程均为6周,随访6个月.结果 观察组发热、多汗、关节痛、肝脾及淋巴结肿大等消失时间较短,与对照组...