法莫替丁与H1受体拮抗剂联合治疗慢性荨麻疹124例疗效观察

124例单用H1受体拮抗剂治疗疗效差的慢性荨麻疹患，联合应用法莫替丁治疗4周，结果显示临床痊愈率和显效分别为85．4％和10．5％，表明法莫替丁与H1受体拮抗剂联合用于这类H1受体拮抗剂不能奏效的慢性荨麻疹患是一种有效的治疗方法。     

Differentiation of human epidermal stem cells into fibroblasts induced by TGF-β1 in vitro

Objective To investigate the correlation between human epidermal stem cell (hESCs) and hypertrophic scar or keloid. Methods Improved collagen Ⅳ-coated adhesion methods was used to isolate and culture the epidermal stem cells after neutral protease selectively digested the dermo-epidermal junctions. After the cells were cultured and expanded in vitro, and passage 3 hESCs were induced by different concentrations of TGF-β1 (0.1, 5.0, and 10.0 ng/ml). Morphological fea-tures and identification of these cells were meseasured by HE, Masson, immunohistochemical staining on the days 3 and 7, respectively. Results After induced by TGF-β1 for 3 and 7 days, the morpholo-gy of the epidermal stem cell (hESCs) was changed into fusiform shape, similar to fibroblasts. 70 % ofthe cell which was induced by TGF-β1 were blue stained in the cytoplasm by Masson stain, which is the distinctive method for collagen, suggesting collagen appeared or increased in the cells. The collagen concentrations in supernatants of hESCs were 0.4150±0.0014, 0.3380±0. 0020, and 0.3870±0.0020, much higher than that in control group (0.0780±0.0025) and normal skin fibro-blast group (0.15004±0.0051) (P<0.05). Immunohistochemical staining revealed that positive rates of these cells for anti-vimentin staining were more than (95.00±1.20)% in experiments and (5.70±0.20)% in control group. Conclusion The differentiantion of hESCs induced by TGF-β1 into fibro-blasts indicates that hESCs may play a role in the pathogenesis of hypetrophic scar and keloid.     

以高钙性肾病为突出表现的原发性甲状旁腺功能亢进1例

以高钙性肾病为突出表现的原发性甲状旁腺功能亢进１例大连医科大学附属第二医院肾内科（１１６０２３）尹永红麻军患者男，４２岁。以多饮、多尿２年，恶心、呕吐３个月为主诉入院。２年前出现烦渴、多饮，每日饮水量７５００～１００００ｍｌ，多尿、夜尿增多。未介意。...     

松节油合成的杀虫增效剂

杀虫增效剂是指对昆虫没有或者很少有杀虫效果的一类化学品,但当它与某些杀虫剂混合使用,能提高杀虫剂的药效,从而达到减少杀虫剂用量,降低成本,减少环境污染的目的。同时,随着长时间的用药,许多害虫对一些杀虫剂均有不同程度的抗性,加入增效剂也是减少抗性的手段之一。 松节油是松脂加工后得到的萜烯类物质,     

移植肾切除67例次报告

移植肾切除６７例次报告郝俊文李慎勤李香铁杨德安刘少鸽王洪伟韩伟我院于１９８６年３月～１９９５年１２月共施行尸肾移植３６９次，其中移植肾切除６７例次，移植肾切除率为１８１％。现就移植肾切除的原因、术后并发症及有关问题进行回顾性分析，报告如下。临床资料...     

颅内肿瘤术后死亡原因分析

颅内肿瘤术后死亡原因分析李书成，郝维杰（沈阳第一临床学院神经外科，沈阳１１０００１）关键词脑肿瘤；死亡率；分析回顾性分析我科１９８５年９月以来施行颅内肿瘤手术１３５０例，其中死亡４２例，死亡率为３％左右，作者对其死亡原因作了初步分析，以期总结经验教训...     

犬环杓后肌电起搏参数

双侧喉返神经(RLN)损伤可致气道梗阻,选择性切除喉内组织或声带外移恢复呼吸功能的几种手术均不太理想。有报道对喉返神经麻痹采用电刺激喉内肌改善喉功能,但电起搏的许多参数尚不清楚,作者介绍一组试验并确定一组参数。将杂种成年犬分成两组,Ⅰ组RLN紧急切断术(对照组),Ⅱ组RLN切断加静脉注射巴夫龙(0.1mg/kg),旨在消除对神经的直接刺激。分离暴露环杓后肌(PCA),将两个特制的针电极间距2mm平行插入PCA,垂直于肌纤维,电极间所产生的椭圆形电场与肌纤维     

褪黑素和哌替啶对小鼠的镇痛作用比较

利用热板法或甩尾法测定小鼠痛阈，发现褪黑素１０～４０ｍｇ·ｋｇ－１ｉｐ后６０ｍｉｎ时镇痛作用最强，１５０ｍｉｎ时仍有镇痛作用。哌替啶１０～４０ｍｇ·ｋｇ－１ｉｐ后３０ｍｉｎ时镇痛作用最强，１２０ｍｉｎ时已无镇痛作用。在６０ｍｉｎ时两者镇痛作用相近。是示两者的作用特点不同。