神经生长因子及其受体在人胰腺导管癌组织中的表达

Objective To investigate nerve growth factor (β-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma. Methods Expression and distribution of β-NGF, tyrosine kinase A (TrKA) and P75NGFR were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of β-NGF and its receptors with clinicalpathological characters, especially nerve invasion were analyzed. Results β-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0. 01). Β-NGF and TrKA expression are associated with the differentiation grades(DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of β-NGF and TrKA. Β-NGF and TrKA expression have positive correlations. Β-NGF, TrKA and P75NGFR mRNA expression have significantly increased 3.84,4. 23 and 2. 41 times than normal tissues by real-time PCR, respectively. Conclusions β-NGF and TrKA might play potential rules in carcinogenesis for pancreatic cancer,have affinity with clinicopathological characters of pancreatic cancer. Β-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.     

原发肾脏恶性淋巴瘤7例并文献复习

目的:探讨原发肾脏恶性淋巴瘤(PRL)的发病率、临床特征、转移规律、治疗方法及影响预后的因素.方法:回顾性分析2000年1月至2009年1月收治的7例PRL患者的资料,收集性别、年龄、症状、体征、病理类型、B超、CT、PET-CT检查等资料并分析不同治疗方法对生存的影响及预后相关因素.结果:2000年1月至2009年1月间天津市平均人口数中PRL的发病率为6.48/1 000万.7例PRL患者中男3例,女4例,年龄10～72岁,中位年龄55岁.首发部位以单侧肾脏多见.4例以腰痛或血尿就诊,2例在常规体检中发现肾脏肿物,1例以低热、盗汗就诊.4例行肾脏切除术,3例行肾脏肿物穿刺活检.病理均为非霍奇金淋巴瘤(NHL),其中B细胞淋巴瘤(B-NHL)6例,T细胞淋巴瘤(T-NHL)1例.均接受了CHOP或CHOP样方案化疗.男、女性平均生存期分别为9、19.5个月,巨块型(是、否)平均生存期分别为8、24.3个月,体力状态评分(PS)(0、1、2分)平均生存期分别为30.5、10.5和7.7个月,单纯化疗与手术联合化疗的平均生存期分别为8.7、19.8个月.结论:PRL是罕见的结外淋巴瘤.临床和影像学表现不典型,容易误诊为"肾癌",确诊依赖于组织病理学检查.治疗方式推荐手术切除+化疗(放疗)为主的综合治疗.预后可能与性别、肿瘤大小、PS评分和治疗方式有关.     

原发性乳腺恶性淋巴瘤的发病特征和诊疗分析

目的 探讨原发性乳腺恶性淋巴瘤(PBL)的发病率、高发年龄和发病趋势、影像学特点、转移规律及不同治疗方法对预后的影响.方法 总结1960年1月至2007年8月收治的49例PBL患者的资料,收集性别、年龄、病理类型、乳腺钼靶X线及B超检查、转移部位等资料并分析治疗与预后的相关性.结果 1960年至2007年间天津市平均人121数中PBL的发病率为59/1000万,高发年龄30～59岁;自1960年至2007年每10年间该病的发病率分别为2/1000万、3/1000万、0、13/1000万和32/1000万,非霍奇金淋巴瘤占绝大部分(48例),乳腺钼靶X线、B超、冰冻病理检查不典型,随访显示脏器受累以骨髓(9/49)、肺(7/49)、脑膜(4/49)和卵巢(4/49)多见,5年总体生存率仅为6.1%.根治术+化疗组患者预后均优于单纯肿物切除术+化疗组和全乳或区段切除术+化疗组(5年存活率分别为21.4%、0、0).结论 PBL是一组罕见的结外淋巴瘤,发病率逐年增加,临床及影像学表现不典型,易累及骨髓、肺、脑膜、卵巢,治疗方案以根治术联合化疗及局部放疗为佳,但预后不良.     

Neuropilin-1在胰腺导管癌及MIA PaCa-Ⅱ细胞系中的表达及意义

目的探讨Neuropilin-1在胰腺导管癌组织及MIAPaCa-Ⅱ胰腺癌细胞系中的袁达及意义。方法运用免疫组化和RT—PCR法分别检测正常胰腺组织、癌旁组织、胰腺癌组织及MIAPaCa-Ⅱ细胞系中Neuropilin-1蛋白及mRNA表达水平。结果蛋白水平可见正常胰腺组织无表达,癌旁组织轻度表达,而胰腺癌组织及MIAPaCa-Ⅱ胰腺癌细胞中高水平表达。神经组织也可表达Neuropilin-1。mRNA水平见正常胰腺组织微量表达,癌旁组织中度表达,而胰腺癌组织及MIAPaCa-Ⅱ胰腺癌细胞中高水平表达。结论Neuropilin-1可能参与了胰腺癌的发生发展,在胰腺癌神经转移中可能起着重要作用。     

TAM与VEGF在外周T细胞淋巴瘤非特指型中表达及临床意义

  目的  探讨肿瘤相关巨噬细胞(TAM)及血管内皮生长因子(VECF)在外周T细胞淋巴瘤非特指型(PTCL-NOS)中的表达及临床意义。  方法  采用免疫组织化学方法对60例PTCL-NOS患者肿瘤组织中的CD68和VEGF进行检测, 15例正常人淋巴结组织为对照。  结果  肿瘤组织中CD68阳性细胞平均含量为(56.5±18.6)个/高倍镜视野, 而对照组为(12.4±6.2)个/高倍镜视野(P< 0.01), 肿瘤组织与对照组VEGF阳性表达率分别为78.3%和26.7%(P< 0.05)。TAM与骨髓侵犯、IPI评分及疗效相关(P< 0.05)。TAM高表达组和低表达组的2年总生存率(overall survival, OS)分别为23.6%和55.3%(P< 0.05)。VEGF的表达与肿瘤分期、骨髓侵犯和IPI评分相关(P & lt; 0.05), VEGF表达阳性组和阴性组的2年OS分别为22.9%和83.3%(P< 0.01)。单变量生存分析显示VEGF表达、TAM计数、肿瘤分期、IPI评分和疗效是独立的预后影响因素(P< 0.05)。多变量分析显示VEGF和疗效是独立的预后影响因素(P< 0.05)。  结论  TAM和VEGF在PTCL-NOS中表达明显升高, 单因素分析显示二者是PTCL-NOS的不良预后因素。多因素分析显示仅VEGF是独立的预后影响因素。      

神经生长因子及其受体在人胰腺导管癌组织中的表达

Objective To investigate nerve growth factor (β-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma. Methods Expression and distribution of β-NGF, tyrosine kinase A (TrKA) and P75NGFR were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of β-NGF and its receptors with clinicalpathological characters, especially nerve invasion were analyzed. Results β-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0. 01). Β-NGF and TrKA expression are associated with the differentiation grades(DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of β-NGF and TrKA. Β-NGF and TrKA expression have positive correlations. Β-NGF, TrKA and P75NGFR mRNA expression have significantly increased 3.84,4. 23 and 2. 41 times than normal tissues by real-time PCR, respectively. Conclusions β-NGF and TrKA might play potential rules in carcinogenesis for pancreatic cancer,have affinity with clinicopathological characters of pancreatic cancer. Β-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.     

神经生长因子及其受体在人胰腺导管癌组织中的表达

Objective To investigate nerve growth factor (β-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma. Methods Expression and distribution of β-NGF, tyrosine kinase A (TrKA) and P75NGFR were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of β-NGF and its receptors with clinicalpathological characters, especially nerve invasion were analyzed. Results β-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0. 01). Β-NGF and TrKA expression are associated with the differentiation grades(DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of β-NGF and TrKA. Β-NGF and TrKA expression have positive correlations. Β-NGF, TrKA and P75NGFR mRNA expression have significantly increased 3.84,4. 23 and 2. 41 times than normal tissues by real-time PCR, respectively. Conclusions β-NGF and TrKA might play potential rules in carcinogenesis for pancreatic cancer,have affinity with clinicopathological characters of pancreatic cancer. Β-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.     

全胰切除治疗胰腺癌

探讨胰腺癌术式的选择。方法自１９９７年８月－１９９８年２月对３例胰腺癌患者全胰切除术。结果３例患者术后恢复为顺利，无严重合并症发生。除１例术后６个月死于肝转移，另２例随访至５个月和９个月仍健在，无转移征象，生活质量良好。     

胆管类癌32例并文献复习

目的探讨肝外胆管类癌的临床特征和临床诊治。方法报告2例胆总管类癌并结合文献报道30例;对32例肝外胆管类癌进行分析。结果肝外胆管类癌临床少见,32例中女性23例,男性9例,发病年龄平均为49岁(19～79)岁。临床表现梗阻黄疸最为常见,占55%。肿瘤局限于胆管内为71%,诊断时己有远处转移的为29%,其肿瘤局限于胆管内病例3年存活率为100%。结论肝外胆管类癌与胆管腺癌不同,其发病年龄年轻(平均为49岁),并好发于女性。肿瘤的局部浸润为主,很少发生远处转移,根治切除后可获长期生存,对胆管类癌应行积极的根治切除。