131Ⅰ治疗Graves病血清细胞凋亡分子的变化及其相关性研究

通过Graves病(GD)患者131I治疗前后可溶性细胞凋亡相关蛋白(sFas)、肿瘤坏死因子-α(TNF-α)及可溶性肿瘤坏死因子受体2(sTNFR2)的含量变化,研究131I治疗与细胞凋亡的相关性及影响.用EIA法和RIA法监测41例GD患者131I治疗前后sFas、TNF-α和sTNFR2水平变化,根据治疗后6至8个月的临床效果,分为缓解组27例和未缓解组14例.结果表明,治疗前,缓解组和未缓解组血清sFas、TNF-α和sTNFR2水平与对照组比较差异有显著性(P＜0.05);治疗后,缓解组血清sFas、TNF-α和sTNFR2水平与对照组比较差异无显著性(P＞0.05),未缓解组与对照组比较差异仍有显著性(P＜0.05,P＜0.01).治疗前与治疗后比较,缓解组血清sFas、TNF-α和sTNFR2差异均有显著性(P＜0.01),未缓解组差异则无显著性(P＞0.05).作者认为,131I产生的电离辐射对患者甲状腺细胞凋亡过程产生一定影响,血清sFas、TNF-α和sTNFR2的含量变化可以作为GD患者131I治疗临床观测和预后判断的良好指标.     

METABOLIC DERANGEMENT OF GLUCOSE, BILE ACID AND AMINO ACIDS AFTER PORTASYSTEMIC SHUNTS IN CIRRHOTIC RATS

Carbon tetrachloride was used to induce cirrhosis in 81 Wistar rats. The animals were divided into three groups to receive end-to-side mesocaval shunt (MCS), end-to-side portacaval shunt (PCS) and Sham operation as control, respectively. Dynamic values of blood glucose and hormones for regulating glucose metabolism, SGPT, serum glycine conjugated cholic acid (CCA) and free serum amino acids were determined and compared with those obtained from 10 normal rats. The determinations suggested that the insulin level depended on the severity of hepatocellular damage with hyperglucagonemia after portasystemic shunt, and CCA was markedly elevated in MCS rats after interruption of the enterohepatic circulation. Changes of CCA were also influenced by hyperglucagonemia and poor liver function in PCS rats. Increase in the aromatic amino acids (AAA) level was related to the impaired hepatic function and hyperglucagonemia. However, decrease in the branched-chain amino acids (BCAA) level was not likely in response to the serum insulin level. Therefore, it is concluded that imbalance of serum amino acids has partial relationship with the derangement of glucose metabolic hormones.     

STUDIES OF EFFECTS OF PORTASYSTEMIC SHUNT ON THE FUNCTION OF HEPATIC AND PANCREATIC ISLET CELLS

The present study was aimed at dynamic observation of the ef fects of end to side portacaval shunt (PCS) and end to side mesocaval shunt (MCS) in dogs on the functions of the liver and pancreatic islet cells. According to correlation between the changes of plasma insulin level in the portal vein and hepatic flow and liver morphology after PCS and MCS, we conclude that the depletion of hepatic flow is the major factor in the deterioration of liver functions. The levels of insulin and glucagon in both the peripheral vein and the portal vein were decreased after PCS and MCS. There was also depletion of pancreatic islet A and B cells and vacuolar degeneration of the pancreas. These changes were more signifcant in PCS than in MCS, suggesting that portasystemic shunt, especially total portasystemie shunt, might damage pancreatic endocrine functions.     

双核素标记维生素B_(12)吸收试验——104例测定结果分析

应用~(57)Co和~(58)Co分别标记内因子结合态和游离态维生素B_(12)吸收试验,检测了萎缩性胃炎74例,胃手术后13例,回肠切除术后1例,晚期血吸虫病10例和正常人6例,其中31例恶性贫血呈维生素B_(12)吸收障碍,有15例仅示~(58)Co吸收低下,16例则示~(57)Co和~(58)Co均吸收不良,各为10.5±6.2％和4.22±2.78％(正常人为23.1±1.21％和24.1±2.66％);4例全胃切除者~(58)Co吸收严重障碍(0.08±0.15％),而6例血清维生素B_(12)水平低下的胃大部切除后病人维生素B_(12)吸收正常。另同时检测血清维生索B_(12)和内因子浓度、内因子抗体,据此分析和讨论了各种因素和维生素B_(12)吸收障碍的关系。     

血清与支气管分泌液中癌胚抗原测定对肺癌的诊断价值

目前认为癌胚抗原测定对肺癌的辅助诊断、疗效评价与判断预后有一定的临床意义。我院从1985年以来测定病人的血清CEA值与肺癌局部支气管分泌液的CEA值,以非肺癌组作对照,探讨对临床诊断的价值。     

新生儿(脐血)血清铁蛋白分析

婴儿生下后的初几个月中,因母奶或牛奶中含铁量都很低,所以胎儿时期体内的储存铁是生后合成血红蛋白的重要来源。孕妇的血清铁是胎儿所需铁的唯一来源,胎儿和胎盘对孕妇血循环中的铁,具有特殊的摄取能力。我们开展放射免疫分析法测定血清铁蛋白,为测定新生儿体内储存铁的研究,开辟了一个新的途径。     

妊娠高血压综合征胎儿免疫状况的测定

从近代免疫观点看来,妊娠可以说是同种异体移植,而妊娠之所以能继续,则有赖于母一婴之间一系列防排斥机制所确立的免疫平衡。一旦平衡失调,可能导致病理情况。有证据表明妊娠高血压综合征可能与母体胚胎抗原负载过大和保护性抗体(系IgG)产生不足有关。伴有尿蛋白的妊娠高血压综合征患者,在肾脏和子宫螺旋小动脉之血管内皮或基底膜上,可见 IgG、IgM 和 C_3之沉着。胎儿可以逃脱免疫的损伤,一方面由于胎盘之屏障可使进入胎体内的母体 T 细胞停止分裂,丧失其攻击能力,另一方面由于胎儿本身产生一些免疫抑制因子,其中较为重要的是甲胎蛋白(AFP)而     

健身跑对中老年男性激素分泌的影响

以３０名系统参加健身跑４年以上，每天或隔天坚持锻炼不少于５～６ｋｍ以上，年龄在５０岁以上的男性中老年人作为研究对象，以无器质性心肺疾患，身体健康，无烟酒嗜好，从不参加运动的５０岁以上男性中老年人（２２例）作为对照组。主要测定激素有：生长激素（ｈＧＨ）、促甲状腺释放激素（ＴＳＨ）、促黄体生成激素（ＬＨ）、卵泡刺激激素（ＥＳＨ）及甲状腺素（Ｔ３、Ｔ４）、可的松（Ｃｏｒｔｉｓｏｌ）、胰岛素（Ｉｎｓ）、睾酮（Ｔ）、雌二醇（Ｅ２）及第二信史ｃＡＭＰ、ｃＧＭＰ。各项检测指标均采用放射免疫分析法测定。结果表明，在内分泌系统各层次激素浓度两组之间ＦＳＨ、Ｉｎｓ无显著差异，而ｈＧＨ，ＬＨ，Ｅ２。Ｔ，ＴＳＨ，ＦＴ３，Ｃｏｒｔｉｓｏｌ，ｃＡＭＰ及ｃＧＭＰ水平，健身长跑中老年组有明显变化，两组有非常显著差异（Ｐ＜０．００１），ＦＴ４有显著差异（Ｐ＜０．０５）．提示长期坚持系统健身跑，能使不同层次内分泌功能得到改善与提高，内分泌反馈调节作用得到加强。