A quantitative method of measuring the microleakage of thermocycled or non-thermocycled posterior tooth restorations

This study quantified microleakage in restorations made with three packable resin composites-Solitaire, SureFil and P60; one hybrid resin composite-Z250 and an amalgam-Dispersalloy, with or without the thermocycling process. Sixty sound, freshly extracted human molars were sectioned mesiodistally, creating buccal and lingual blocks. One hundred blocks with the flattest surface were selected. Cylindrical cavities with a diameter of 1.85 +/- 0.05 mm and a depth of 1.5 mm were prepared with a special diamond bur. The blocks were randomly assigned to 10 test groups (n = 10): five restorative materials and two thermal stress groups (thermocycled groups at 3,000 cycles at 5 degrees C and 55 degrees C with a dwell time of one minute at each temperature, or non-thermocycled). After the thermocycling test, the samples were immersed in 2% methylene blue for 12 hours. The samples were ground and the powder prepared for analysis in an absorbance spectrophotometer. All the results were statistically analyzed by the Kruskal-Wallis test and the Mann Whitney test. For the non-thermocycled groups, the means (microg/ml) of microleakage were: Amalgam-4.279 (a); Solitaire-4.148 (ab); Z250-3.418 (abc); P60-3.184 (bc); SureFil-2.890 (c). For the thermocycled groups, the means were: Amalgam-7.572 (a); Solitaire-5.471 (a); Z250-4.330 (ab); P60-3.418 (bc) and SureFil-2.779 (c). Thermocycling analysis showed no significant differences between the thermocycled and non-thermocycled groups for each material tested. It was concluded that no test material prevented microleakage. Only SureFil and P60 showed leakage means significantly lower than amalgam, with SureFil showing lower leakage than Solitaire. P60 only showed lower leakage than Solitaire in the thermocycled groups and Z250 showed results similar to the others materials.     

Treatment of gingival fibromatosis associated with Zimmermann-Laband syndrome

BACKGROUND: Gingival fibromatosis is a rare condition characterized by a generalized enlargement of the buccal and lingual aspects of the attached and marginal gingiva. METHODS: This case report describes the periodontal management of a 13-year-old female patient with gingival fibromatosis associated with Zimmermann-Laband syndrome. The patient presented with gingival enlargement involving the maxillary and the mandibular arches, anterior open bite, and non-erupted teeth. Periodontal treatment included gingivectomy in all four quadrants. RESULTS: Histopathologic evaluation of the excised tissue supported the diagnosis of gingival fibromatosis. A significant improvement in esthetic appearance and eruption of the non-erupted teeth were obtained. The patient was referred for appropriate orthodontic treatment and has been closely followed for the earliest signs of recurrence of gingival enlargement. CONCLUSIONS: The successful therapy for gingival fibromatosis depends on correctly identifying the etiological factors and improving the impaired function and esthetic appearance through surgical intervention and adjunctive orthodontics. Maintaining treatment results depends on preservation of periodontal health.     

下颌复合组织移植实验的显微外科解剖学基础研究

同种异体下颌区复合器官和组织移植的研究刚刚起步,为了了解下颌区复合器官和组织移植的显微外科解剖基础,在犬、兔和猴进行了下颌区及颈部血管解剖比较研究。结果发现,在下颌区域,颌外动脉、颌内动脉、颌外静脉和颌内静脉形成闭合的循环回路。在此基础上可以确定下颌复合器官移植的范围并使移植手术得以进行。     

同种异体神经行面神经缺损修复的初步应用

目的:探讨化学去细胞同种异体神经移植修复人体面神经缺损的效果。报告3例同种异体神经成功修复面神经缺损的体会。方法:对3例面神经损伤,应用化学去细胞同种异体神经移植,修复神经缺损,术后定期对患者进行运动功能检查。结果:所有患者在同种异体神经后无异常反应,切口愈合良好。1名患者术后9个月运动功能部分恢复。结论:化学去细胞神经同种异体移植修复人体面神经缺损,能够恢复神经功能,避免了切取自体神经的弊端,在临床上应用是可行的。     

切断双侧腭大动脉的上颌骨前份截骨术的血管造影观察

长期以来,对于是否一定要保留腭大动脉以维持上颌骨前份截骨段的血供尚无一致意见。软组织切口的设计关系到术后局部软、硬组织的血液供应,有时还与截骨段能否充分移动有关。本研究利用线血管造影方法观察在切断双侧腭大动脉并在唇内侧做弧形切口条件下,恒河猴上颌骨前份截骨术前后的上颌骨截骨段血供来源变化。结果发现,术后截骨段血供最初来自由唇动脉等血管构成的血管网,尔后,当鼻腔面粘骨膜再附着后,也可为截骨段提供一定量的血供。我们认为不仅可以切断双侧腭大动脉,而且还可以在唇侧做切口,使唇腭侧截骨操作都可在直视下进行,同时使截骨段获得充分的移动。这将特别有利于腭裂术后病人的上颌骨外科正畸手术。     

局部应用二磷酸盐对鼠正畸牙移动影响的研究

目的 探讨局部注射Zoledronate溶液对鼠正畸牙齿移动距离与牙周组织形态的影响。方法 选用42只雄性Wistar大鼠，牵引其上颌第一磨牙近中移动。实验中分别将Zoledronate溶液及生理盐水注射入实验组（左侧）及对照组大鼠（双侧）上颌第一磨牙腭侧的粘骨膜下。注射于实验前3d开始，共进行9次，每3d一次。分别在加力0、3、7、14、21d后记录上颌第一磨牙移动距离，组织HE染色后，观察牙周组织形态学的改变。结果 ①实验组大鼠牙齿移动距离明显低于对照组。②实验组大鼠压力侧破骨细胞数在实验全过程中均低于对照组，而根分叉区破牙骨质细胞数除加力14d外，2组差异无显著性。③实验过程中Zoledronate溶液对破骨细胞和破牙骨质细胞以外的细胞作用不明显。结论 Zoledronate能有效地抑制支抗牙移动，减少压力侧牙槽骨表面破骨细胞数。     

Immunolocalization of a human cementoblastoma-conditioned medium-derived protein

Little is known about the molecular mechanisms that regulate the cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as a cementum biological marker, we studied its expression and distribution in human periodontal tissues, human periodontal ligament, alveolar bone, and cementoblastoma-derived cells. In human periodontal tissues, immunoreactivity to anti-CP was observed throughout the cementoid phase of acellular and cellular cementum, cementoblasts, cementocytes, cells located in the endosteal spaces of human alveolar bone, and in cells in the periodontal ligament located near the blood vessels. Immunopurified CP promoted cell attachment on human periodontal ligament, alveolar bone-derived cells, and gingival fibroblasts. A monoclonal antibody against bovine cementum attachment protein (CAP) cross-reacted with CP. These findings indicate that CP identifies potential cementoblast progenitor cells, is immunologically related to CAP species, and serves as a biological marker for cementum.     

颌面部神经内分泌癌五例

颌面部神经内分泌癌比较少见 ,可为原发的 ,也可为转移性的 ,我们收治颌面部神经内分泌癌患者 5例 ,其中原发性 2例 ,转移性 3例 ,男性 2例 ,女性 3例 ,平均年龄 6 9岁。均接受头颈部和胸部CT检查 ,其中 3例行颈部和腹部MRI检查 ,2例接受全身正电子发射体层显影 ( positronemissiontomography ,PET)检查。随访时间为 6～ 30个月。2例原发性神经内分泌癌患者中 ,1例初次手术切除范围在肿瘤周缘 2cm者 ,术后 4个月局部复发 ,接受第 2次扩大切除手术 ,在肿瘤周缘 3cm距离扩大切除直至皮下组织 ,随访观察 2 0个月未见复发。另 1例术后接受放…     

Viral RNA splicing and papillomavirus oncogenesis

Human papillomavirus (HPV) infection is an etiologic cause leading to development of cervical and other ano-genital cancers as well as a subset of oropharyngeal cancers.     

Plaque inhibitory effect of a 0.05% cetyl-pyridinium chloride mouth-rinse in a 4-day non-brushing model

To cite this article:
Int J Dent Hygiene 9 , 2011; 266–273 DOI: 10.1111/j.1601‐5037.2010.00490.x
García V, Rioboo M, Serrano J, O′Connor A, Herrera D, Sanz M. Plaque inhibitory effect of a 0.05% cetyl‐pyridinium chloride mouth‐rinse in a 4‐day non‐brushing model. Abstract: Objectives: Results from clinical studies evaluating the efficacy of the adjunctive use of cetyl‐pyridinium chloride (CPC) containing oral hygiene products have shown wide variability, probably due to differences in formulations. The objective of this study was to determine the inhibitory plaque effect of a 0.05% CPC mouth rinse in de novo plaque formation in a 4‐day non‐brushing experimental model. Materials and methods: The study was designed as a short‐term double‐blind randomized cross‐over experimental model aimed to compare three products: a negative control (similar to the test product, without active ingredients), a positive control (with 0.12% chlorhexidine and CPC) and the test product (with 0.05% CPC) in terms of plaque index, gingival inflammation and microbiological variables. Results: Plaque levels after 4 days were 2.88 for the positive control, 3.86 for the negative control and 3.60 for the test. Differences among groups on day 4 were statistically significant (P < 0.001). Gingival index showed comparable values at baseline (P = 0.745), and significant increases were observed, with the exception of the positive control. Total colony forming units showed comparable values at baseline (P = 0.125) and significant increases were observed only in the negative control. Conclusions: The tested 0.05% CPC mouth‐rinse is capable of inhibiting plaque formation.