新型脊髓完全横断缺损模型大鼠的建立

BACKGROUND: Rat models of complete spinal cord transection are common models for neural tissue engineering. After transecting the spinal cord by the previous methods, gap length of broken end cannot keep relatively uniform, so we cannot objectively evaluate effects of various treatments or tissue engineering materials. OBJECTIVE: The spinal cord transection models were established by using double edged micro scissors, and the feasibility of this new model was explored by comparing with the conventional method. METHODS: A total of 42 adult female Sprague-Dawley rats were divided randomly into group A (n=6), group B (n=18) and group C (n=18). Group A only received laminectomy. In the group B, the spinal cord was transected with a sharp-pointed knife. Knife point should touch anterior wall of spinal canal and sidewall bone surface. Complete spinal cord transection models were prepared by repeated cutting. In group C, complete spinal cord transection models were established by using self-made double edged micro scissors. RESULTS AND CONCLUSION: (1) At 1 week after model establishment, in the groups B and C, complete paralysis of the hind limbs was found, and BBB scores were similar. However, significant differences in the spacing of broken end were detected. (2) At 4 weeks after model establishment, hind limb functions could restore to different degrees in both groups, but no significant difference in BBB scores was found. (3) At 8 weeks after model establishment, significant differences in hindlimb motor function scores were detectable between both groups. Biotin glucosamine tracer display: In group B, a few labeled axon fibers were observed at the caudal side of the injured spinal cord. In group C, spinal cord was completely transected, and labeled axon fibers cannot be found at the caudal side. (4) Results suggested that the modeling method of self-made double edged micro scissors could effectively eliminate individual differences, contribute to quantitative analysis and comparative study of therapeutic effects.      

糖痹康对糖尿病模型大鼠坐骨神经胰岛素样生长因子Ⅰ及其受体表达的影响

BACKGROUND: Chinese medicine Tangbikang can improve nerve conduction velocity in diabetic rats, and has anti-inflammatory and antioxidant effects. Insulin like growth factor-I is a key target in the treatment of diabetic peripheral neuropathy. OBJECTIVE: To observe the effect of Tangbikang on the expression of insulin-like growth factor-I and its receptor protein and mRNA in the sciatic nerve of diabetic rat model. METHODS: The experimental diabetes mellitus rat models were induced by feeding high fat forage and injection with streptozotocin. After model establishment, rats were given Tangbikang 4.18, 8.35, 16.7 mg/kg per day. This study set positive control methycobal, model and normal control groups. Intragastric administration was performed for 16 weeks. RESULTS AND CONCLUSION: Compared with the model group, blood glucose levels were similar in the methycobal group, but decreased in the high-dose Tangbikang group (P < 0.01). Immunohistochemical staining and real-time fluorescence quantitative PCR detection revealed that body mass, motor nerve conduction velocity, insulin like growth factor-I and its receptor protein and mRNA expressions were increased in the methycobal and high-dose Tangbikang groups (P < 0.05 or P < 0.01). Results indicated that Tangbikang can prevent and treat diabetic peripheral neuropathy by promoting insulin like growth factor-I and its receptor.       

针刺太冲和足三里治疗高血压病临床观察

目的观察针刺太冲、足三里治疗高血压病的临床疗效。方法临床确诊高血压病患者46例,按自愿、双盲原则分为治疗组和对照组,治疗组每天针刺太冲、足三里,早晚各1次;对照组给予安慰剂口服,一次2片,每天2次。两组每个疗程均为7d,观察患者的血压下降水平。2个疗程后分别观察其疗效,观察两组血压变化。结果治疗组在针刺治疗后血管紧张素转换酶（ACE）表达逐渐升高,在第14天时升高最明显,且明显高于对照组,对照组则无明显升高;与治疗后相比两组收缩压与舒张压均下降,而与对照组相比治疗组经治疗后舒张压下降更明显。结论针刺太冲、足三里能有效降低高血压病患者的血压。     

八珍汤在骨科病人手术后应用体会

现代创伤病人多由于高能量致伤,多为复合伤,损伤较大,失血较多.术后患者功能恢复欠佳,且易合并发热、烦躁、出汗、失眠、体倦、乏力等不适症状.近年来,笔者于创伤病人术后给以八珍汤加减治疗,效果满意,现报道如下:     

“不死之癌”——强直性脊柱炎

<正>颈椎病、腰椎间盘突出症、脊柱侧弯是大家较为熟知的脊柱疾病,但近年来,有一种与脊柱相关的风湿免疫性疾病,因其症状痛苦,伴随终身难愈的特点,“粗暴地”走入人们的视野,更因此被称为脊柱的“癌症”。面对这种无法扭转的疾病,“强直”患者该如何缓解疼痛呢？老百姓常说：“人未老,背先老。”腰酸背痛是现代人很常见的病症,一般表现为忙碌后加重,休息几天便能够得到有效的缓解。然而,有一种背痛持续时间长,越休息,身体反而越僵硬,     

急性脊髓损伤患者早期血清标志物与AISA分级的相关性

背景：目前临床针对急性脊髓损伤严重程度的评估方法存在一定局限性,亟需一种快速、精准的评估手段。目的：分析急性脊髓损伤患者血清内相关氧化应激、神经损伤指标表达量与疾病严重程度的相关性。方法：选择2020年8月至2022年5月濮阳市中医医院收治的患者65例,其中急性脊髓损伤患者32例(试验组),单纯脊柱骨折患者33例(对照组),入院8 h内,评估患者美国脊髓损伤协会(ASIA)残损分级及神经功能评分,采集静脉血样本来检测血清中超氧化物歧化酶、丙二醛、谷胱甘肽、一氧化氮、神经胶质纤维酸性蛋白和神经元特异性烯醇化酶的水平,分析上述血清指标与ASIA残损分级、神经功能评分的相关性。结果与结论：(1)与对照组相比,试验组患者血清超氧化物歧化酶、谷胱甘肽水平降低(P <0.001),丙二醛、一氧化氮、神经胶质纤维酸性蛋白和神经元特异性烯醇化酶水平升高(P <0.01);(2)Pearson或Spearman相关分析显示,试验组患者血清中超氧化物歧化酶、谷胱甘肽水平与AISA残损分级呈正相关(r=0.862 4,0.849 3,P <0.01),丙二醛、神经胶质纤维酸性蛋白、神经元特...     

中西医结合强化理疗方案治疗选择性脊神经后根切断术后肌力下降的疗效分析

目的探讨中西医结合强化理疗方案治疗脊神经后根切断术(SPR)后肌力下降的疗效。方法采用运动疗法、理疗、电针治疗及中医手法相结合的方案治疗SPR术后肌力下降28例(治疗组);采用单纯运动疗法治疗16例(对照组)。对比术后当日、术后2周患儿小腿主要肌肉肌力。结果治疗2周后,治疗组患儿肌力高于对照组(P<0.05),并恢复至术前水平(P>0.05)。结论中西医结合强化理疗方案可以使患儿SPR术后肌力快速恢复。     

川芎-丹参配伍治疗脊髓损伤的网络药理学研究与验证

目的：基于网络药理学,预测川芎-丹参配伍治疗脊髓损伤的作用机制。方法：利用网络药理学工具,获得川芎-丹参治疗脊髓损伤的有效成分、核心靶点及信号通路。采用改良Allen重物打击法构建脊髓损伤模型,采用酶联免疫吸附试验（ELISA）测定各组大鼠活性氧（ROS）、丙二醛（MDA）水平;采用蛋白质印迹法检测各组大鼠脊髓组织中磷脂酰肌醇3激酶（PI3K）、p-PI3K、蛋白激酶B（AKT）、p-AKT蛋白表达。结果：化合物-靶点网络包含154个化合物和484个相应靶点;基因本体（GO）功能富集分析得447个条目;京都基因和基因组百科全书（KEGG）通路富集分析得到126条信号通路。动物实验结果表明,与假手术组比较,模型组ROS、MDA水平显著增高（P<0.01）;p-PI3K、p-AKT蛋白表达显著下降（P<0.01）;与模型组比较,中药高剂量组ROS、MDA水平显著下降（P<0.01）;p-PI3K、p-AKT蛋白表达显著增高（P<0.01）。结论：川芎-丹参联合治疗脊髓损伤具有多成分、多靶点、多通路的优势,其作用机制可能与调控PI3K/AKT信号通路有关。     

匙羹藤对胰岛素抵抗小鼠脂肪组织蛋白激酶B 的影响

目的：观察匙羹藤对胰岛素抵抗（IR）KKAy 小鼠脂肪组织中蛋白激酶B 表达及磷酸化的影响以及调节机制。方法：将18只胰岛素抵抗KKAy小鼠按体质量随机分为模型组（DM）和匙羹藤水提物组（GS）,并选取9只C57BL/6J小鼠为正常对照组（NC）,连续灌胃给药8周。实验结束后取血测定空腹血糖（FPG）,空腹血清胰岛素（Fins）,并计算胰岛素敏感指数（ISI）,测定附睾脂肪组织中磷酸激酶依赖的激酶1（PDK1）、蛋白激酶B（PKB）、P-PKB（Ser473）,P-PKB（Thr308）蛋白相对含量,及磷酸酶和张力蛋白同源物基因（PTEN）mRNA 相对含量。结果：与DM 组相比,GS组小鼠FPG、Fins降低,ISI升高,P-PKB（Ser473）蛋白相对含量及其磷酸化水平升高,P-PKB（Thr308）蛋白相对含量升高,PDK1蛋白相对含量降低,PTENmRNA表达量降低。结论：匙羹藤可通过增加脂肪组织中P-PKB（Ser473）蛋白相对含量,增强Ser473位点磷酸化水平,以及增加P-PKB（Thr308）蛋白相对含量激活PKB,进而改善IR。     

中西医结合治疗下肢深静脉血栓形成56例临床观察

目的:总结下肢深静脉血栓形成的中西医结合治疗经验。方法:对56例下肢深静脉血栓形成患者进行回顾性分析。应用尿激酶、脉络宁注射液静脉滴注,同时辨证服用中药汤剂,外用消肿止痛散。结果:临床治愈率达65.5%,总有效率为97.3%;彩色超声多普勒动态观察,股静脉再通率达91.4%,静脉再通率达99.7%,小腿静脉丛再通率达62.3%,血液粘度降低。结论:以溶栓、活血化瘀为主的中西医结合综合疗法治疗下肢深静脉血栓形成疗效满意,可促使静脉复通,血液粘度降低。