Claudin 4在人月经周期子宫内膜的表达

目的:探讨Claudin4在人月经周期子宫内膜的定位和表达与子宫内膜容受性的形成和胚泡着床的关系。方法:收集33例正常育龄期妇女子宫内膜,用荧光实时定量PCR检测Claudin4mRNA在月经周期各期子宫内膜的表达。用免疫组织化学方法和图像分析方法对Claudin4蛋白在子宫内膜中的表达进行定位和半定量分析。结果:Claudin4mRNA和蛋白在人月经周期子宫内膜呈连续动态表达,增殖早期表达极低,随后逐渐上升,在分泌中期表达明显高于其他各期(P<0.05),分泌晚期表达下降。Claudin4蛋白主要表达于子宫内膜腺上皮、腔上皮。结论:Claudin4在分泌中期子宫内膜的高表达可能与子宫内膜容受性的形成和胚泡着床的过程有关。     

带锁髓内钉治疗复杂骨折122例临床观察

由于带锁髓内钉具有固定牢固、抗旋转、适应症广、骨折愈合率高以及并发症少等优点,越来越受到广大医务人员的重视,并深为病人所接受。我院自2003年元月至今,共应用带锁髓内钉治疗复杂骨折122例,收效良好,现总结如下。1一般资料本组122例中,男79例,女43例;年龄最大56岁,最小19岁,平均32岁;股骨中下段粉碎折44例,其中多段骨折26例;胫骨中下段粉碎折78例,多段骨折18例;闭合性骨折79例,开放性骨折43例;陈旧性骨折延迟愈合32例,新鲜骨折90例;行闭合复位穿针内固定28例,小切口切开复位内固定94例;住院时间最长29d,最短15d,平均19d。2治疗方法以股…     

中药加透明质酸钠治疗髌骨软骨软化症

髌骨软骨软化症又称髌骨软骨病,是髌骨软骨面及其相对的股骨髌面的关节软骨退行性变,是膝关节痛的常见原因,多见于女性和运动员,起病缓慢,多为原发性.笔者自2002年3月～2004年3月2年间,运用中药熏蒸加透明质酸钠关节内注射治疗本病46例,54个膝,效果良好.现总结分析如下.     

糖痹康对糖尿病模型大鼠坐骨神经氧化应激、线粒体能量代谢及AMPK/SIRT3通路的影响

目的 探讨糖痹康对糖尿病坐骨神经病变的影响及可能作用机制。方法 60只雄性SD大鼠随机分为正常组、模型组、硫辛酸组和糖痹康高、中、低剂量组,每组10只。除正常组外,其余各组大鼠采用高脂高糖饲料饲养+链脲佐菌素腹腔注射建立糖尿病大鼠模型。造模成功后硫辛酸组大鼠予硫辛酸注射液0.0268 g/(kg·d)进行腹腔注射,糖痹康高、中、低剂量组分别予糖痹康颗粒剂2.5、1.25、0.625 g/(kg·d)灌胃,正常组和模型组予生理盐水10 ml/kg灌胃,均连续干预12周。各组分别于给药前和给药4、8、12周检测血糖、体质量;给药12周后,检测大鼠热痛阈与坐骨神经运动神经传导速度(MNCV),取大鼠坐骨神经检测氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)]、炎症因子[白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)]、线粒体能量代谢指标[烟酰胺腺嘌呤二核苷酸(NAD⁺)、还原型辅酶Ⅰ(NADH⁺)、三磷酸腺苷(ATP)]水平;HE染色检测大鼠坐骨神经切片组织形态,电镜观察大鼠坐骨神经超微结构;检测大鼠坐骨神经腺苷酸活化蛋白激酶...     

护患沟通在急诊科护理工作中的重要性

目的探析急诊科医疗服务中护患沟通的重要性。方法选取2016年6月至2016年12月间我院未实行护患沟通管理政策时急诊科收治的50例病患为对照组,选取2017年1月至2017年6月我院采用护患沟通政策时急诊科收治的50例病患为观察组,比较两组患者对护理的满意度和护患纠纷事件发生的几率。结果观察组患者对护理的满意度明显高于对照组(P0.05),观察组护患纠纷事件的发生率显著低于对照组(P0.05)。结论采用护患沟通护理,急诊科患者对护理的满意度大大提高,有效地减少了护患之间的矛盾,提升了护理工作的效率。     

经皮电刺激改善气滞血瘀型骨折患者中医证候的效果评价

目的 探讨经皮电刺激疗法改善气滞血瘀型骨折患者中医证候的效果。方法 选取2021年2—12月北京中医药大学东直门医院收治的气滞血瘀型骨折患者60例,按随机数字表法分为对照组30例（常规手术治疗）与电刺激组30例（常规手术治疗联合电刺激疗法）。比较治疗前后两组患者中医证候积分、中医证候疗效评定、肢体功能评分与日常生活能力评分。结果 手术前,两组中医证候积分差异无显著性（P>0.05）。治疗后,电刺激组的中医证候积分明显低于对照组（P<0.01）。对照组中医证候疗效评定总有效率为73.33%,电刺激组总有效率为100%,差异有显著性（P<0.01）。治疗前、治疗后对照组与电刺激组患者肢体运动功能评分与日常生活能力组间比较结果显示,两组差异无显著性（P>0.05）。结论 电刺激疗法可显著改善气滞血瘀型骨折患者的中医证候,提高中医证候疗效,但对肢体运动功能与日常生活能力的改善与对照组差别不大。     

川芎嗪对脊髓损伤大鼠铁死亡相关分子表达的影响

目的研究川芎嗪对脊髓损伤大鼠铁死亡相关分子表达的影响, 探讨其促进脊髓损伤修复的相关机制。方法将36只SD大鼠按随机数字表法分为假手术组、模型组和川芎嗪组, 每组12只。假手术组大鼠行椎板切除术, 不损伤脊髓, 其余2组制备脊髓损伤大鼠模型, 川芎嗪组大鼠术后腹腔注射川芎嗪注射液80 mg/kg, 假手术组和模型组腹腔注射等体积生理盐水, 1次/d, 连续干预28 d。分别于术前1 d和术后1、3、5、7、14、21、28 d采用BBB肢体运动功能评分评价大鼠肢体运动功能。采用尼氏染色观察神经元形态, 采用普鲁士染色观察铁沉积, 采用试剂盒检测脊髓组织MDA、活性氧(ROS)含量, 采用Western blot法检测脊髓组织谷氨酸-半胱氨酸反转运系统轻链(xCT)、谷胱甘肽过氧化物酶4(GPX4)和长链脂酰辅酶A(ACSL4)蛋白表达, 采用qPCR法检测脊髓组织xCT、GPX4、ACSL4 mRNA水平。结果术后第14、21、28天, 与模型组比较, 川芎嗪组BBB肢体运动功能评分升高(P<0.01);川芎嗪可显著改善神经元形态结构, 降低脊髓组织中铁含量;与模型组比较, 川芎...     

新型脊髓完全横断缺损模型大鼠的建立

BACKGROUND: Rat models of complete spinal cord transection are common models for neural tissue engineering. After transecting the spinal cord by the previous methods, gap length of broken end cannot keep relatively uniform, so we cannot objectively evaluate effects of various treatments or tissue engineering materials. OBJECTIVE: The spinal cord transection models were established by using double edged micro scissors, and the feasibility of this new model was explored by comparing with the conventional method. METHODS: A total of 42 adult female Sprague-Dawley rats were divided randomly into group A (n=6), group B (n=18) and group C (n=18). Group A only received laminectomy. In the group B, the spinal cord was transected with a sharp-pointed knife. Knife point should touch anterior wall of spinal canal and sidewall bone surface. Complete spinal cord transection models were prepared by repeated cutting. In group C, complete spinal cord transection models were established by using self-made double edged micro scissors. RESULTS AND CONCLUSION: (1) At 1 week after model establishment, in the groups B and C, complete paralysis of the hind limbs was found, and BBB scores were similar. However, significant differences in the spacing of broken end were detected. (2) At 4 weeks after model establishment, hind limb functions could restore to different degrees in both groups, but no significant difference in BBB scores was found. (3) At 8 weeks after model establishment, significant differences in hindlimb motor function scores were detectable between both groups. Biotin glucosamine tracer display: In group B, a few labeled axon fibers were observed at the caudal side of the injured spinal cord. In group C, spinal cord was completely transected, and labeled axon fibers cannot be found at the caudal side. (4) Results suggested that the modeling method of self-made double edged micro scissors could effectively eliminate individual differences, contribute to quantitative analysis and comparative study of therapeutic effects.      

糖痹康对糖尿病模型大鼠坐骨神经胰岛素样生长因子Ⅰ及其受体表达的影响

BACKGROUND: Chinese medicine Tangbikang can improve nerve conduction velocity in diabetic rats, and has anti-inflammatory and antioxidant effects. Insulin like growth factor-I is a key target in the treatment of diabetic peripheral neuropathy. OBJECTIVE: To observe the effect of Tangbikang on the expression of insulin-like growth factor-I and its receptor protein and mRNA in the sciatic nerve of diabetic rat model. METHODS: The experimental diabetes mellitus rat models were induced by feeding high fat forage and injection with streptozotocin. After model establishment, rats were given Tangbikang 4.18, 8.35, 16.7 mg/kg per day. This study set positive control methycobal, model and normal control groups. Intragastric administration was performed for 16 weeks. RESULTS AND CONCLUSION: Compared with the model group, blood glucose levels were similar in the methycobal group, but decreased in the high-dose Tangbikang group (P < 0.01). Immunohistochemical staining and real-time fluorescence quantitative PCR detection revealed that body mass, motor nerve conduction velocity, insulin like growth factor-I and its receptor protein and mRNA expressions were increased in the methycobal and high-dose Tangbikang groups (P < 0.05 or P < 0.01). Results indicated that Tangbikang can prevent and treat diabetic peripheral neuropathy by promoting insulin like growth factor-I and its receptor.       

纳米材料支架与骨髓间充质干细胞构建纳米骨修复兔股骨头坏死

背景:清华大学材料系制备的纳米晶胶原基骨修复材料的化学性能和物理性能均符合人体内环境要求,具有良好的生物相容性.目的:利用纳米材料和组织工程技术,探索骨髓间充质干细胞和纳米晶胶原基骨修复材料在骨坏死修复中的效果.方法:制备骨髓间充质干细胞与纳米骨复合体.取46只新西兰大白兔建立双侧股骨头坏死动物模型,随机分为3组.假手术组不做任何治疗;单纯纳米骨植入组植入纳米骨;纳米骨+骨髓间充质干细胞组植入复合了干细胞的纳米骨.对植入后4,8,12周的股骨头行影像学观察以及组织学观察.结果与结论:X射线检查:植入后12周,假手术组兔股骨头有塌陷;单纯纳米骨植入组充填区与周围组织区别不明显,周边有新生骨小梁;纳米骨+骨髓间充质干细胞复合体植入组充填区近似周围组织,充填区分布有骨小梁.组织学观察:4周时,假手术组股骨头坏死区无明显变化,其他两组植入物降解和新骨替代;8周时,假手术组股骨头负重区软骨面部分缺损,其他两组坏死区初步修复,可见成骨和材料降解;12周时,假手术组坏死区仍未修复,部分股骨头有塌陷,其他两组坏死区修复,纳米骨+骨髓间充质干细胞植入组骨小梁结构形成.结果表明,单纯纳米骨植入组和纳米骨+骨髓间充质干细胞植入组在股骨头坏死成骨方面效果优于假手术组,纳米骨+骨髓间充质干细胞植入组成骨更佳.