颈淋巴结活检后的鼻咽癌22例疗效分析

本文报道２２例颈淋巴结活检后的鼻咽癌患者，放疗结束时颈淋巴结残留占３１．８％，颈部照射野处皮肤湿性反应占４０．９％，远处转移率高达７７．２％，放疗反应重，效果差，５年生存率仅为１８．２％。而５６例经鼻咽原发灶活检的鼻咽癌放疗效果好，五年生存率达５３．１％。本文提示，鼻咽癌经颈淋巴结活检是放疗失败的重要原因之一。     

变异型心绞痛的发作诱因和合并症

本文报告3例饮酒诱发变异型心绞痛者,2例发展为急性心肌梗塞,1例合并Ⅲ度房室传导阻滞.另3例无诱因的典型变异型心绞痛发作皆无合并症.上述结果提示:饮酒是变异型心绞痛发作的最常见诱因,与无诱因者相比,予后更为严重.因此,有冠状动脉痉挛倾向者如变异型心绞痛,不稳定型心绞痛,卧位或夜间发作的心绞痛等,应避免饮酒.     

儿童输尿管炎性假瘤一例报告

泌尿生殖系炎性假瘤 (ＩＰＴ)少见 ,我们收治 1例 ,报告如下。患儿 ,男 ,9岁。因肉眼血尿 1个月 ,右侧腰痛 1周 ,伴轻度排尿刺激症状 ,于 2 0 0 1年 1 1月 8日就诊。无泌尿系统阳性体征。尿脱落细胞学检查可见异形细胞。Ｂ超示右输尿管下段实性占位 ,凸入膀胱 ,右肾重度积水。ＩＶＵ右肾 60ｍｉｎ未显影 ,膀胱右后壁可见充盈缺损。ＭＲＩ示右输尿管下段梭形占位 ,凸入膀胱 ,未侵犯周围组织。ＭＲＵ示右肾重度积水 ,膀胱图像与ＩＶＵ近似。诊断 :右侧输尿管下段占位性病变。硬膜外麻醉下探查 ,术中冰冻切片提示炎性肿物 ,但切除后输尿管缺损…     

慢性稳定性心绞痛患者肺炎衣原体感染检测的意义

目的　探讨肺炎衣原体感染与慢性稳定性心绞痛患者血中炎症标志物之间的关系。方法　采用微量免疫荧光法 (MIF)和酶联免疫法 (ELISA)对 5 2例经冠状动脉造影证实的慢性稳定心绞痛患者 (CSA)血中肺炎衣原体IgG抗体 (CpIgG)和肿瘤坏死因子 α(TNF α)、白细胞介素 6 (IL 6 )、C 反应蛋白 (CRP)进行检测。结果　在慢性稳定性心绞痛患者组中CpIgG阳性率为 5 5 .77% ;而CpIgG滴度与TNF α、IL 6和CRP单因素及多因素回归分析发现只有CpIgG与TNF α显著相关 (P <0 .0 5 ) ,但与IL 6和CRP之间无显著相关性 (P <0 .0 5 )。在慢性稳定性心绞痛患者中肺炎衣原体感染可能促进TNF α的升高 ,但肺炎衣原体的感染不是导致斑块不稳定的主要因素     

李有伟谈“有毒”中药在痹证中的应用

芜湖市中医医院李有伟主任从事痹证临床研究多年，对于“有毒”中药在痹证治疗中的应用颇有心得，笔者有幸师从，现将学习所得归纳总结如下。     

除去白细胞的输血

负责研讨血液安全的日本厚生劳动省专家调查会4月 2 3日决定 ,将在日本启动除去白细胞的输血。由于白细胞可成为输血后急性肺功能障碍等重症副作用的原因 ,英国、法国、德国、加拿大等国已 1 0 0 %地实施了除白细胞输血 ,美国也在引入这一机制。该调查会指出 ,输血用血液除去白细胞后 ,除减少急性肺功能障碍外 ,还可减少细菌感染、发热等副作用。即使万一有变异型雅各布病患者献血 ,也能大幅度降低其危险性。除去白细胞的输血@徐爽 @盛志业     

Analysis of differentially expressed genes in fetal skin of scarless and scar-forming periods of gestational rats

Objective: To study the differences of gene expression between earlier gestational skin and later gestational skin of rats with the aids of single primer amplification (SPA) and high-density oligonucleotide DNA array to understand the molecular mechanism of scarless healing. Methods: Total RNAs were isolated from fetal rat skin of the scarless (E15) and scar-forming (E18) periods of gestation (term =21.5 days). The RNAs from earlier gestational skin (EGS) and later gestational skin (LGS) were both reversely transcribed to cDNAs, then labeled with the incorporation of fluorescent dCTP for preparing the hybridization probes by SPA method. The mixed probes were then hybridized to the oligonucleotide DNA arrays which contained 5 705 probes representing 5 705 rat genes. After highly stringent washing, these DNA arrays were scanned for fluorescent signals to display the differentially expressed genes between the 2 groups of skin. Results: Among 5 705 rat genes, there were 53 genes (0.93%) with differentially expressed levels between EGS and LGS groups, 27 genes, including fibroblast growth factor 2 ( FGF2 ) and follistatin were up-regulated (0.47%) and 26 genes were down-regulated (0.46%) in fetal skin during scarless period versus scar-forming period. Higher expressions of FGF2 and follistatin in EGS than those in LGS were also revealed by RT-PCR method. Conclusions: High-density oligonucleotide DNA array provided a powerful tool for investigating differential gene expression in earlier and later gestational fetal skins. This technology validates that the mechanism of fetal scarless healing is very complicate and the change of many gene expressions is associated with fetal scarless healing.     

核因子κB圈套寡核苷酸减轻大鼠移植肝缺血/再灌注损伤的作用和机制

目的探讨抑制枯否（Kupffer）细胞核因子κB（Nuclearfactor-kappaB，NF-κB）活性对减轻大鼠移植肝缺血／再灌注损伤（IRI）的作用和机制。方法建立大鼠肝移植缺血／再灌注损伤模型。实验分正常对照组、缺血／再灌注组和圈套寡核苷酸组，每组均为8只大鼠。圈套寡核苷酸组于移植术前2d经供者尾静脉注入120μg脂质体包裹的NF-κB圈套寡核苷酸。移植再灌注后2h，取各组受者移植肝分离枯否细胞。凝胶迁移变动分析法（EMSA）检测枯否细胞NF-κB蛋白结合活性，逆转录聚合酶链法（RT—PCR）观察枯否细胞肿瘤坏死因子α（TNF—α）和白细胞介素6（IL-6）mRNA的表达，同时观察肝组织病理及肝功能变化。结果缺血／再灌注组移植肝再灌注后2h，枯否细胞NF-κB活性及TNF-α、IL-6 mRNA表达量较对照组明显升高（P〈0．01）。光镜下肝细胞大量变性、坏死，伴有肝血窦明显淤血，血清丙氨酸转氨酶（ALT）和胆红素总量（TBIL）较对照组明显升高（P〈0．01）。相反，圈套寡核苷酸组枯否细胞NF-κB活性及细胞因子mRNA表达与缺血／再灌注组相比明显下降（P〈0．01），移植肝未见明显病理组织学改变，肝功能明显改善。结论NF-κB圈套寡核苷酸能高效抑制枯否细胞NF-κB活性，并抑制其下游有害细胞因子的产生，从而减轻缺血／再灌注损伤对移植肝的打击和损害。     

肝移植后非肿瘤性疾病复发

肝移植在手术方式、器官保存及免疫排斥的治疗上取得了重大进展,但移植后非肿瘤性疾病的复发,主要包括肝炎、巨细胞病毒感染、自身免疫性肝病等,往往导致移植肝功能失活,受者死亡.因此,肝移植后非肿瘤疾病复发已日益引起重视.     

俞尚德治疗小儿脾胃病验案举隅

小儿稚体娇嫩，易为内外因素所扰，若过食厚味，或乱用补品，易使小儿脾胃功能受损而形成诸多病证。俞尚德老师治疗小儿消化系统疾病，用药轻灵，疗效卓著。现将其所治验案举隅如下。