3 岁以下患儿反复喘息的相关因素研究

目的 探究≤ 3 岁患儿反复喘息的相关因素。方法 选取2015 年6 月—2017 年6 月齐齐哈尔医学 院附属第三医院收治的523 例喘息患儿作为研究对象,所有患儿年龄均≤ 3 岁,依据患儿喘息发作次数将其分 为反复喘息组（喘息次数≥ 3 次）和对照组（喘息次数≤ 2 次）。回顾性分析两组患儿临床资料,单因素和 Logistic 多因素分析≤ 3 岁患儿反复喘息的相关因素。结果 经单因素方差分析,两组患儿出生方式、纯母乳喂养 时间、家族过敏史、其他个人过敏史、血清免疫球蛋白E（IgE）、感冒次数、血嗜酸细胞（EOS）、胎龄、体重 增长、食物过敏史、湿疹、被动吸烟、饲养宠物及肺炎史比较差异有统计学意义（P <0.05）;经Logistic 多因素 进一步分析,剖宫产、纯母乳喂养时间≤ 4 个月、有家族过敏史、有其他个人过敏史、血清IgE>60 IU/ml、感 冒次数>6 次、血EOS>5%、有湿疹、有被动吸烟、有饲养宠物、有肺炎史为≤ 3 岁儿童反复喘息的危险因 素（P <0.05）。结论 应对具备危险因素的≤ 3 岁反复喘息患儿加强监控,以期降低其反复喘息的发生率。     

结肠梗阻1例报告

<正> 肠梗阻作为临床的常见病、多发病,目前仍以X线检查为最直接、有效。临床上小肠机械性梗阻较大肠梗阻更为常见,对前者的诊断,难度不大,但对后者而言,由于病例较少,往往容易误诊,现就我科遇到的1例乙状结肠不全梗阻报告于后。 患者:刘宣增,男,67岁,以“腹泻、呕吐十余天,伴腹胀加重2d”为主诉入院。急诊查体,上腹部饱满,叩诊鼓音,伴     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

白癜风患者外周血CD4+CD25+调节性T细胞的检测

Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.     

面颈部暴发性痤疮1例

1 临床资料 患者男,16岁。面颈部起丘疹、脓疱、结节、囊肿1个月,疼痛伴发热2周,于2012年12月9日来诊,以“暴发性痤疮”收入院治疗。患者1个月前无诱因面颈部突然出现丘疹、脓疱,当时未诊治,皮疹逐渐加重形成囊肿、肿胀,部分表面坏死结血痂。     

应用改良Kugel补片治疗成人马鞍疝的体会

目的:探讨改良Kugel补片治疗成人马鞍疝的技术操作要点,并评价其疗效.方法:回顾性分析我科应用改良Kugel补片修补45例成人马鞍疝的临床资料.结果:45例患者术后恢复顺利,除3例出现阴囊浆液肿外,其余42例均并发症,随访1 ～ 30个月,无腹股沟疝复发,效果满意.结论:应用改良Kugel补片治疗成人腹股沟马鞍疝安全有效,术后恢复快,疗效满意.     

血栓通注射液对晚期血吸虫病肝纤维化患者的疗效及患者肝纤维化指标的影响

目的探讨血栓通注射液对晚期血吸虫病肝纤维化患者的疗效及患者肝纤维化指标影响。方法 120例晚期血吸虫病肝纤维化患者,随机分为观察组(66例)和对照组(54例)。对照组患者给予常规保肝治疗,观察组患者在对照组基础上给予血栓通注射液。比较两组临床疗效、血清生化指标、肝纤维化指标以及不良反应。结果观察组治疗总有效率显著高于对照组(P0.05);两组患者治疗前TBIL、ALT、AST水平比较,差异无统计学意义(P0.05),治疗后两组患者血清TBIL、ALT、AST水平均显著降低(P0.05),且治疗后观察组TBIL、ALT、AST水平显著低于对照组(P0.05);治疗前两组患者肝纤维化指标比较,差异无统计学意义(P0.05),治疗后两组肝纤维化指标均显著降低(P0.05),且治疗后观察组肝纤维化指标显著低于对照组(P0.05)。结论血栓通注射液能够有效提高血吸虫病肝纤维化患者的临床疗效,促进患者肝功能以及肝纤维化病情的改善,用药安全可靠,值得临床推广。     

N-乙酰氨基葡萄糖对皮肤角质形成细胞透明质酸合成的影响

[摘要] 目的 研究外源性N-乙酰氨基葡萄糖（NAG）对体外培养的人皮肤角质形成细胞（HEKa）透明质酸（HA）合成的影响。方法 采用MTT法检测不同浓度NAG对HEKa细胞增殖的影响;ELISA方法检测不同浓度NAG对HEKa细胞HA合成的影响;RT-PCR法检测NAG对HEKa细胞透明质酸合成酶（即HAS1、HAS2和HAS3）mRNA表达的影响。结果MTT结果表明,1mM/L、3mM/L和5mM/L的NAG对HEKa细胞的增殖无明显影响（P>0.05）,而10 mM/L的NAG显著抑制HEKa细胞的增殖（P<0.05）;ELISA 结果显示,NAG呈剂量依赖性促进HEKa细胞HA的生成（P<0.01）,最佳浓度为5mM/L;RT-PCR结果显示,NAG对细胞HAS1、HAS2和HAS3mRNA的表达无明显促进作用。结论 外源性NAG具有促进体外培养的HEKa细胞合成HA的作用,而对HAS mRNA的表达无明显影响。