纳洛酮联合无创正压通气治疗老年COPD并发呼吸衰竭的临床疗效

目的:研究NIPPV联合纳洛酮治疗呼吸衰竭的效果.方法:研究资料为2013.1~2016.12我院治疗的 96例老年COPD患者,联合使用NIPPV疗法与纳洛酮治疗本组老年患者.结果:96例中显效68例,有效21例,无效7例,有效率为92.71%.结论:联合应用纳洛酮及NIPPV疗法可以有效治疗出现呼吸衰竭症状的老年 COPD患者.     

山东海警某部官兵皮肤病发生情况的调查

目的了解山东海警部队官兵皮肤病的发生情况,为海警部队皮肤病防治工作提供科学依据。方法整群抽样选取山东威海地区海警某部官兵作为调查对象,根据官兵患病后的就诊登记、战后总结及上报材料情况,由门诊医生进行认真详细填写,统计各种常见皮肤病患病人数。各类皮肤病的诊断标准和依据均按照《皮肤性病学》诊断。利用构成比和发病率进行相关统计分析。结果山东海警部队官兵2016-2018年皮肤病发生率均较高,总体变化趋于平稳态势,发病率分别为70.20%、78.19%、74.00%。皮肤病发生有一定季节规律,其中第三季度发病率高于其它季度。三年间皮肤病总发病数为813人,其中蚊虫叮咬所占比例最高,为26.81%,其次为湿疹和脚癣,分别占比18.94%和14.64%。病种构成也有明显的季度特征。结论山东地区属于黄海,冬季寒冷干燥,夏季温暖而潮湿,四季气候变化较大,山东海警的皮肤病发生有季节变化规律,皮肤病防治工作应该随着季节变化而有所侧重;海上作业风吹日晒,气候潮湿,蚊虫叮咬和真菌性皮肤病严重,是防范的重点内容;作业强度大,居住密集,湿疹和各类皮炎发生率也比较高,另外,海警人群年龄普遍较较轻,痤疮毛囊炎等也不容忽视。     

骨髓间充质干细胞促进肺癌转移的机制

BACKGROUND:So far the positive or negative effects of mesenchymal stem cells on tumor growth and metastasis are under discussion. OBJECTIVE:To explore the mechanism of bone marrow mesenchymal cells in promoting lung cancer metastasis. METHODS: Primary rat bone marrow mesenchymal stem cells were obtained by direct adherence method of the whole bone marrow, and differential adherence combined with digestion control method was performed to purify cells. Lung cancer cell lines were cultured, and the effects of bone marrow mesenchymal stem cells on the migration, invasion and metastasis of lung cancer cells were observed by scratch test, cell invasion and migration assays. Orthotopic lung cancer models were established in rats and bone marrow mesenchymal stem cells were seeded onto the left lung of rats. Then, pathological changes of lung tissues were observed at 14 days after transplannation. RESULTS AND CONCLUSION:After the scratch test, the migration rate of lung cancer cells became higher, and the scratches healed with time. And after cell transplantation, the number of migrated lung cancer cells increased, as well as the ability of lung cancer cells penetrating the Matrigel was strengthened. Besides, fibrous connective tissues could be found around the lung cancer tissues, and necrosis with distinct boundary and large tumor nuclei; the metastatic tissues showed obvious infiltration and necrosis with large tumor nuclei. These results suggest that bone marrow mesenchymal stem cells can promote the invasion, migration and metastasis of lung cancer cell lines.       

人脐带间充质干细胞分离鉴定及移植后对肺癌小鼠化疗的影响

BACKGROUND:Mesenchymal stem cells have pluripotent differentiation, and can promote cell engraftment and immune regulation. Therefore, we attempt to use human umbilical cord mesenchymal stem cells as a new source for treatment of lung cancer by exploring cell isolation, identification and transplantation combined with chemotherapy for lung cancer in mice. OBJECTIVE:To investigate the isolation and identification of human umbilical cord mesenchymal stem cells and its transplantation combined with chemotherapy for lung cancer in mice. METHODS: Human umbilical cord mesenchymal stem cells were isolated from fresh umbilical cord of newborns and identified using tissue culture and enzyme digestion. Twenty Balb/C nude mouse models of lung cancer were randomly divided into two groups: mice in chemotherapy group were given chemotherapy, and those in combined group given combination of chemotherapy with human umbilical cord mesenchymal stem cell transplantation. RESULTS AND CONCLUSION:Compared with the chemotherapy group, the gastrointestinal tract was rosy and shiny, intestinal mucosa was smooth and complete, and tumor mass and blood indexes significantly decreased in the combined group (P < 0.05). To conclude, mature human umbilical cord mesenchymal stem cells can be obtained by tissue culture and enzyme digestion, and the cell transplantation combined with chemotherapy can significantly reduce gastrointestinal tract damage and the make peripheral hemogram in a stable level.      

羊布鲁杆菌脂蛋白OMP19单克隆抗体制备与鉴定

目的 制备与鉴定羊布鲁杆菌脂蛋白OMP19单克隆抗体,用于布菌感染免疫机制研究。方法 将OMP19基因连接入pET-30a(+)表达载体中,构建pET-30a(+)/OMP19质粒,转化入大肠埃希氏菌BL21(E3),以不同浓度异丙基-β-D硫代半乳糖苷(IPTG)进行诱导表达,采用镍金属螯合亲和层析(NI-NTA)纯化;以布菌阳性血清检测重组蛋白免疫反应性,并利用杂交瘤技术制备单克隆抗体,以天然OMP19蛋白及布菌外膜蛋白提取物(NMP)对制备的单克隆抗体进行Western Blot及酶免疫染色试验(IEST)鉴定。结果 表达了OMP19蛋白,分子量约19 kDa,通过纯化纯度可达95%,Western Blot分析显示蛋白具有良好的抗原性,并制备了OMP19抗原23株鼠源性单克隆抗体,鉴定结果 显示22(95.65%)株能与天然OMP19蛋白反应,18(78.26%)株能与NMP反应,其中IgG1(k)亚型占91.30%;4株能与羊种布鲁氏菌菌涂片反应。结论 成功制备具有良好抗原性的重组OMP19蛋白,筛选出识别天然蛋白的单克隆抗体,已初步应用于布菌的检测,为OMP19抗原B细胞表位的筛选奠定基础。     

动态载荷下单端桥基牙牙周膜应力的三维有限元分析

并不明显.结论 在下颌后牙单端桥的咀嚼运动中,侧向耠力对基牙牙周健康影响最大;修复后对近缺隙侧基牙的牙周组织条件要求更高;连续多个动态载荷周期加载下牙周膜形成的应力积累不会对牙周膜造成损害,修复第二磨牙缺失的双基牙单端桥设计是可行的.     

爆炸性武器破片与冲击波复合伤发生情况初探

目的 探讨爆炸性武器破片与冲击波复合伤发生情况和损伤特点。方法 山羊34只,用装药量2k和30k的战斗部进行静爆,观察现场至伤后24小时动物存活情况,形态学改变和破片与冲击波复合伤的发生率。结果 现场死亡15只,伤后24小时内死亡3只,死亡率为18／34(52．94％)。形态学改变主要表现为软组织挫伤或缺损,体腔破裂,肢体断离缺失,肢体骨折,心、肺、胃肠道、肝、肾等内脏器官不同程度出血,甚至发生肺破裂,胃肠道穿孔和肝、肾破裂。破片与冲击波复合伤的发生率为16／34(47．06％)。结论 爆炸性武器所致破片与冲击波复合伤具有发生率高、伤情重,死亡率高的特点,早期救治的重点是及时有效地处理肺损伤和内脏器官破裂与穿孔。     

微生物学及免疫基础课堂教学问卷调查分析与研究

微生物学及免疫基础是医学生必修的基础课程。通过对我院2004、2005、2006级及2007级514名学生微生物学及免疫基础课堂教学个人需求问卷调查及效果问卷调查情况的反馈信息的分析,找到了课堂教学中所存在的问题和不足,及时改变以往的教学模式,并提出相应的教学改革及措施,使课堂教学取得了较好的效果,以期达到培养和提高学生综合能力与素质的目标,为创新人才的培养开辟一条新的途径。