Expression of 5α-Reductase Type 2 Gene in Human Testis, Epididymis and Vas Deferens

Objectives To study the expression pattern of 5α-reductase type 2 gene in human male reproductive organsMethods The expression level of 5α-reductase type 2 gene inhuman testis, epididymis and vas deferens tissues was determined by in situ hybridization using Digoxin labeled 5α-reductase type 2 cRNA probe.Results The brown granules of hybridizing signals distributed in the cytoplasm of Sertoli and Leydig cells of the testis, the principle cells of epididymis and the epithelial cells of vas deferens, but there was no positive signal in the nuclei of above-mentioned cells. No positive signal was observed in germ cells, basement of the testis, interstium of epididymis and basement, as well as smooth muscle of vas deferens. Conclusion This study confirmed that the 5α-reductase type 2 gene expressed in Sertoli , Leydig cells of the testis, and the principle cells of epididymis. The expression pattern of the gene in these cells in human was similar to that of rat and monkey. The presence of 5α-reductase t     

阴式全子宫切除术130例临床评价

子宫切除术是妇科常见的手术之一,近几年来,随着微创手术引入妇科领域,阴式全子宫切除术再次受到重视[1]。因它不必做腹部切口,手术时间短,术中出血少,术后患者起床活动早,住院时间短,副反应少。本院从1999年8月至2000年12月进行了130例阴式全子宫切除术,均取得了良好的效果。现报告如下。     

小腿后部各区穿支间的关系与皮瓣设计

Objective To study the anastomotic relationships of perforators in each zone of the poste-rior leg and design perforating flaps for clinic. Methods Six fresh cadavers underwent a whole body, intra-arterial injection of a lead oxide and gelatine preparation. The posterior part of leg is divided into upper, mid-die and below equal parts, Observe topography of the perforating branches in every district by layer, and mea-sured their location, diameter, course, branches and anastomosis pattern. Radiographs of tissue specimens were digitally analyzed. Results There were 13 perforators that diameter≥ 0.5 mm, the average external diameter was 0.8 mm. The areas of each perforator supplied was average 38 cm2. Perforators from popliteal artery was identified an area 4 cm wide, around the intersection of two lines, a line drawn between the medial and lateral epicondyles of the femurs, and the midline of posterior leg. The areas of the every perforator sup-plied was 55 cm2. These vessels were large in diameter and create multiple true anastomoses with the perfora-tors from the posterior tibial artery or fibular artery. Perforating branches were small in the below part, a long perforator chain comprised of two to three perforators accompanies the Achilles tendon. Conclusion The perforator flaps deviced by perforators from the posterior leg may be transplanted to the lower limbs and the other part of the body. The perforators located in the middle zone of the leg are larger. Free posterior tibial or peroneal perforator-based flaps are reliable, relatively large, and have thin flaps. The upper and lower zones were the larger donor site for the proximal or distally pedicled perforator flap harvest.     

听骨链多层螺旋CT曲面重建及临床应用

目的研究听骨链的CT曲面重建(CPR)方法,评估其临床应用价值。方法165耳(122例)行颞骨轴位高分辨率CT检查,对听骨链行CPR。50例(80耳)为正常听骨链,72例(85耳)为病变组。主要扫描参数:准直0.5mm,螺距0.875,重建间隔0.2~0.3mm,重建矩阵1024×1024。结果(1)正常听骨链组:1幅图像上清晰显示3个听骨及其关节。(2)临床应用:39例颞骨外伤中,听骨链异常的18例;锤砧关节半脱位及脱位各5例,砧镫关节半脱位及脱位分别为5例及6例,锤骨柄骨折1例。16例外耳发育不良中,骨性外耳道闭锁13例,膜性3例。骨性外耳道闭锁最常见的伴随听骨畸形是锤骨柄发育不全(10例),中耳腔变窄、听骨缺如3例,锤砧融合伴砧骨长突缺如1例。不伴随先天性外耳发育不良的听骨畸形2例,1例为双耳砧骨长突缺如,另1例为砧骨长突发育不全伴砧镫关节不连。慢性中耳乳突炎15例,6例合并胆脂瘤者听骨均有不同程度的破坏,鼓室硬化症1例。结论听骨链的多层螺旋CT曲面重建是诊断传导性耳聋的有效方法。     

行气活血清热利湿法治疗下肢深静脉血栓形成

下肢深静脉血栓形成 (ＤＶＴ)是常见的周围血管疾病。为深讨下肢深静脉血栓形成综合征阶段病人的治疗效果 ,我院自 1999年 10月至 2 0 0 0年 12月应用中药以行气活血、清热利湿的方法进行治疗 ,取得了较好的疗效 ,现将结果报告如下。1　临床资料本组患者均为我院住院病人 ,符合中华全国中医学会脉管专业委员会 1990年 7月制定的下肢深静脉血栓形成的中医诊断标准。选择病程在 4周以上 ,无肺栓塞及肢体溃疡并发症者共 30例 ,35条肢体。其中男 13例 ,女 17例 ;年龄 2 9～ 80岁 ,平均5 8 1岁。病程 2 8ｄ～ 2年 9个月 ,平均 91 7ｄ。正常对照组…     

小肠神经纤维瘤致消化道反复出血2例报告

小肠出血占下消化出血的 1% - 5 % ,小肠肿瘤占消化道肿瘤的 3%左右 ,小肠神经纤维瘤致消化道反复出血则属少见 ,现将我院收治的 2例报告如下 :1　临床资料例 1:女 ,67岁 ,37年前发现血压升高 ,1980年诊断为“高血压” ;1997年 - 2 0 0 1年间数次因“黑便”住院 ,经内科输血、止血等治疗处理 ,症状消失出院。2 0 0 2年 1月因“高血压”住入我院治疗 ,住院 2天又出现黑色稀大便 ,一天十数次 ,继之出现乏力、嗜睡、血压下降。经急救处理病情稳定后 ,结肠镜检未发现病变。CT发现中上腹区软组织肿块影 ,提示小肠肿瘤。肠系膜上动脉造影见 :左中…     

胆系疾病的实验室检查

<正> 胆系疾病实验室检查的目的在于:(1)判断有无胆汁郁积;(2)鉴别肝内型和肝外型胆汁郁积;(3)查明胆系损害的病因;(4)判断有无合并肝损害(见表1)。     

髋关节置换术应用丙烯酸骨粘固剂的麻醉体会

我们将丙烯酸骨粘固剂（简称骨粘固剂）用应于人工孵关节置换手术。所用材料为天津产聚甲基丙烯酸甲脂（Potymethy emethacrg late，简称PMMA）即共聚份与甲基丙烯酸甲脂（Metbry lmethacry eate，简称MMA）。因单体对机体有毒性作用，尤对心血管的影响较为明显，再加髋关节手术组织创伤大，出血多，易发生休克，因此加强手术中监护与麻醉管理十分重要。现将我们的体会报告如下。     

膀胱肿瘤的高温治疗

早在一百多年前就有人观察到丹毒引起的人体高热可使所患的肉瘤消失,以后人们主动地应用人体全身和局部高温来治疗肿瘤。但由于当时技术条件的限制,使这一方法的研究及应用未能进一步深入和推广。随着科学技术的发展,人们对加温的方法及温度的控制有了更多更好的掌握,并利用人工培养的肿瘤细胞来研究高温治疗肿瘤的效果和机理,这样使得这一方法又重新得到重视并广泛地用于临床治疗各种肿