神经营养因子对认知功能障碍的治疗前景

神经营养因子是一类特殊的蛋白质,鉴于其表现出特异的神经营养活性,人们试图用于治疗某些神经-精神疾病。本文对神经营养因子作用机理的研究作简要介绍,并对以Alzheimer氏症为代表的认知功能障碍性疾病的脑内病因及神经生长因子防治作用的实验研究与临床试用进行了评述。     

高寒山区粮食作物玉米赤霉烯酮污染状况调查报告

高寒山区粮食作物玉米赤霉烯酮污染状况调查报告内蒙古哲里木盟卫生防疫站（０２８０００）宝金彩宋宝才李静朱秀王雅宾周海峰朱少兵（指导）哲里木盟扎鲁特旗霍林郭勒乡、哈尔淖和北阿其郎吐村地处东经１２０度，北纬４５°３５′度，海拨５６０ｍ。无霜期９０天，年最低...     

海洛因中毒(附68例报道)

毒品海洛因(俗称白粉),作用与吗啡同,但毒性较吗啡大,自解放以来,毒品中毒原已不曾遇见。但近年来,由于本院地处特区,眦邻港澳,从港澳和国外来诊的人数甚多,现将我院近年来收治的68例海洛因中毒患者报告如下。     

角膜玻璃异物临床观察报告

角膜玻璃异物临床观察报告新乡市中心医院眼科邢志平，吉兰英，李静我们追踪又见察一例角膜有两处玻璃异物病人近几年，现报告如下：患者田ＸＸ，男，２６岁，汽车司机，１９８５年春节期因车祸打碎驾驶室门上玻璃，而致伤右眼及面部，伤处出血较多，眼疼痛及异物感等刺激...     

超声显像对瓣膜腱索断裂的诊断

瓣膜腱索断裂是临床少见的一种心脏急重症,死亡率高。我院自1995～2 0 0 3年经彩色多普勒超声心动图共检出瓣膜腱索断裂2 5例,总结如下。1　对象和方法1.1　对象　本组年龄11～75岁,男18例,女7例,临床表现不同程度劳累性心慌,气短,心前区听到粗糙收缩期杂音,部分病例闻及海鸥鸣音。ECG示房颤7例,左心室肥大伴劳损11例,双室肥大4例,室早3例,右束支传导阻滞2例。胸部X线示两肺不同程度淤血,心脏扩大,心胸比率0 .5 5～0 .75。临床诊断:胸部外伤(车辆、重物撞及胸部) 2例,感染性心内膜炎6例,高心病3例,冠心病2例,慢性风湿性瓣膜病变4例,二尖瓣…     

《刺法灸法学》教学方法改革探索

《刺法灸法学》是以阐述各种针灸技术的操作方法、临床应用及作用原理为主要内容的针灸分支学科，是介于《经络腧穴学》和《针灸治疗学》之间的桥梁课程，是针灸专业的主干与必修课程。通过本课程教学，要求学生掌握临床各种针灸技术的操作方法，熟悉和了解古今各种针灸方法的作用原理和临床应用情况。     

Inhibitory Effects of Anti-sense PTTG on Malignant Phenotype of Human Ovarian Carcinoma Cell Line SK-OV-3

To construct eukaryotic expression vector expressing full length anti-sense pituitary tumor transforming gene (PTTG) mRNA and observe its blocking effect on the potential invasion of human ovarian carcinoma cell line SK-OV-3. PCR primers containing designed enzyme cut sites were used for cloning full-length PTTG gene fragment, and the resulting PCR product was inserted into the eukaryotic vector pcDNA3. 1 in the antisense direction. The recombinant vector was then transfected into SK-OV-3 by Lipofectamine. The positive cell clone was screened by G418, PTTG and bFGF at protein level expression were detected by Western blot. The biological behavior change of transfection positive cells was observed by colony formation in soft agar assay. Our results showed that SK-OV-3 clones stably expressing full-length recombinant pcDNA3. 1-PTTGas were obtained. The expressions of PTTG and bFGF protein in transfected cells were decreased by 61.5% and 52.3%, respectively as compared with non-transfected ones. The number of colony formation was reduced significantly in transfected cells as compared with empty vector transfected and non-transfected cells. It is concluded that the recombinant vector pcDNA3. 1-PTTGas is a novel tool and provides an alternative anti-sense gene therapy targeted at PTTG in human carcinoma.     

血管紧张素转换酶基因与中国人群冠心病、高血压病及糖尿病的关系

目的：研究血管紧张素转换酶(ACE)基因与中国人群冠心病(CHD)、高血压病(EH)及2型糖尿病(T2DM)的关系。方法：250例呈不同组合的EH、T2DM以及CHD患者及90例正常对照用改良的聚合酶链反应(PCR)方法检测ACE基因型，比较基因型及等位基因频率分布。结果：①无合并CHD的T2DM及EH组ACE基因型及等位基因频率与正常对照无显著差异；②CHD组无论是否合并EH及／或T2DM，Ⅲ基因型及Ⅰ等位基因频率均显著低于正常对照，而DD基因型及D等位基因频率均显著高于正常对照；③T2DM合并CHD组及EH合并CHD组中Ⅱ基因型及Ⅰ等位基因频率均显著低于正常对照，而DD基因型及D等位基因频率均显著高于正常对照。结论：ACE基因多态性与中国人群CHD相关。这种关联亦见于EH或T2DM合并CHD中，但ACE基因仅是CHD发病的遗传学基础，而与是否合并EH及／或T2DM无关。     

艾附暖宫颗粒对大鼠子宫平滑肌影响的实验研究

艾附暖宫颗粒对正常大鼠在体内和离体子宫活动均有明显的抑制作用，能对抗催产素引起的大鼠离体子宫的强烈收缩，协同黄体酮对子宫平滑肌的抑制，具有明显的解痉作用。