氧化应激及炎症与糖尿病肾病

糖尿病肾病（ Diabetic nephropathy,DN）是糖尿病最常见、最严重的并发症之一。 DN发病机制复杂,是在遗传背景的基础上多因素综合作用的结果。长期高血糖是导致DN的始动因素,其诱导的氧化应激及炎症反应相互作用,最终导致DN的发生。针对这两个靶点通路的治疗将成为DN治疗领域的新热点。     

屈光不正性弱视类型及初始治疗的年龄对儿童近立体视功能的影响

目的 探讨屈光不正性弱视类型及初始治疗的年龄对近立体视功能的影响.方法 采用颜氏立体图对门诊的121例3~9岁屈光不正性弱视儿童的立体视功能进行治疗前后(6月)的对比分析,观察弱视程度的不同及屈光类型的不同对患儿立体视治疗前后的影响及不同开始治疗年龄对近立体视发育的影响.结果 屈光不正性弱视儿童有不同程度的近立体视,具有正常近立体视锐度比例为22.92%.以散光性屈光不正为主,高度远视者近立体视较差,开始治疗的年龄越小,近立体视功能发育越好,疗效越好,不同年龄组治疗效果间有统计学意义;不同程度的屈光不正性弱视患者治愈后近立体视锐度也存在差异,轻度 、中度弱视较重度弱视立体视功能发育较好,治疗后预后好,重度弱视的立体视功能预后较差,差异有统计学意义.结论 屈光不正性弱视中高度远视对视功能影响较大,开始治疗年龄越小,立体视发育预后较好.开始治疗年龄较晚的立体视发育相对较差.散光型弱视儿童立体视功能明显好于高度远视组儿童(屈光参差除外),重度弱视有一部分视力提高后立体视仍未发育,在弱视治疗的后期要注意双眼视觉的恢复.     

手术室护士术中低体温护理现状与专业认知、安全文化态度的相关性

目的:探讨手术室护士术中低体温护理现状与专业认知、安全文化态度的相关性。方法:选取泰州市人民医院2018年1月至2019年12月在职的手术室护士78名,采用一般资料量表调查护士基础信息,通过自制的手术室术中低体温护理行为量表评价术中低体温护理现状,经Pearson相关性检验分析手术室护士术后低体温护理与专业认知、安全文...     

开胸顺气丸的GC指纹图谱研究

开胸顺气丸由厚朴、陈皮等八味中药组成,具有行气止痛、消积化滞等功效,药典中未制订其含量测定项[1]。本药方成分复杂,测定单一活性成分或指标成分并不能完全反映其质量,制定指纹图谱对其质量标准研究具有重要意义。本药方的活性成分多具有挥发性,能够满足GC法的要求,同时GC法     

硝普钠对大鼠局脑缺血/再灌注后神经型一氧化氮合酶的影响

Objective To investigate the effect of sodium nitroprusside (SNP) on hippocampal neuronal nitricoxide synthase (nNOS)expression after focal cerebral ischemia-reperfusion in rats and the mechanism of neuroprotective effect of SNP. Method 108 male SD rats weighting 250 g-300 g were randomly divided into 3 groups (n=36). Focal ischemia-reperfusion was established by occlusion of middle cerebral artery (MCA ).Control group (C): in which sham operation was performed; Ischemia-reperfusion group (I): after sodium chloride(5 μl) was injected into the lateral cerebral ventricle using microsyringe, MCA was occluded for 2 h; SNP group (S): SNP (0.055 mg/kg, 5μl ) was injected into the lateral cerebral ventricle using microsyringe, then MCA was occluded for 2 h. The experimental groups were further divided into 3 subgroups(n=12) according to the reperfusion time: 2 h, 6 h and 12 h. Neurological function score were tested before reperfusion; Pathological changes were observed by HE staining; The hippocampal tissue were obtained for detection of nNOS protein expression by immuno-histochemistry technique and nNOS mRNA expression by RT-PCR technique. Results Neuronal mortality in every subgroup of group I and S increased significantly compared with that in group C(P<0.05); Compared with that in 2 h(43.8 ± 2.1),6h (73.9±4.7) subgroup of group I, neuronal mortality in 2 h (36.5 ±1.2), 6h (42.6 ± 1.9) subgroup of group S decreased significantly (P<0.05); There were more nNOS mRNA expression in every subgroup of group I and S compared with that in group C (P<0.05),nNOS mRNA expression in 2 h (0.428 3 ± 0.000 4), 6 h (0.482 7 ± 0.005 2 )subgroup of group S decreased compared with that in 2 h (0.472 1 ±0.011 5), 6h(0.744 2±0.011 6)subgroup of group I(P<0.05); The expression of nNOS protein in other subgroup of group I and S increased except that in 6 h subgroup of group S(P<0.05); Compared with that in 2 h(0.265 8 ± 0.000 5), 6 h(0.284 0 ± 0.013 4) subgroup of group I, the expression of nNOS protein in 2 h (0.251 4 ±0.001 1), 6h (0.258 9 ±0.004 0) subgroup of group S decreased (P<0.05). Conclusion SNP coμld attenuate the focal cerebral ischemia/reperfusion injury and the possible mechanism may be related to the inhibition of nNOS expvession.     

基于明清医案探索痿病的用药规律

本研究通过检索《中华医典》中明清医家治疗痿病的医案,获取48位医家的53本著作,共计207篇医案,提取医案中的处方、药物,建立明清痿病用药数据库,对数据进行规范化处理,运用频率分析、关联规则等数据挖掘技术进行研究。结果显示明清医家在治疗痿病的过程中明辨病机虚实,着重从补益精血和清利湿热两个角度入手,在痿病核心方剂的基础上,灵活运用人参-黄芪-白术、黄柏-知母、熟地-龟甲、黄柏-苍术、薏苡仁-桑枝、虎骨-锁阳、牛膝-肉苁蓉等常用药对,虚实兼顾,从而获得较好的治疗效果。