心肌营养素1对谷氨酸诱导神经干细胞凋亡的作用

背景：心肌营养素1属于白细胞介素6细胞因子家族,近年发现其可能对中枢神经系统有重要保护作用。 目的：探索重组腺病毒心肌营养素1对谷氨酸诱导神经干细胞凋亡的保护作用,为脑损伤提供新的治疗措施。 方法：取新生Wistar大鼠海马神经干细胞,第2代培养第5天用于实验,神经干细胞分为3组：谷氨酸组、重组腺病毒心肌营养素1组及对照组,利用四唑蓝比色了解细胞存活情况及原位缺口末端标记技术检测细胞凋亡。 结果与结论：谷氨酸组神经干细胞呈现明显形态改变,重组腺病毒心肌营养素1组未见类似改变,在谷氨酸暴露后12,24,48及72 h MTT吸光度值均低于心肌营养素1组,而细胞凋亡率均高于心肌营养素1组。与其他两组比较,对照组相同时间点A值增高,细胞凋亡率降低。提示,心肌营养素1对谷氨酸损伤的神经干细胞具有保护作用,可以抑制神经干细胞凋亡,促进其存活和增殖。     

骨折远端截骨内固定治疗股骨颈头、头颈型骨折

目的探讨股骨颈头下、头颈型骨折骨折远端截骨内固定治疗的可行性.方法对36例股骨颈头下、头颈型骨折骨折远端进行截骨,缩小原来骨折线的Pauwel角,减少骨折端的剪切应力,增加其压缩应力,将不稳定的股骨颈头下、头颈型骨折变为稳定型骨折,并且通过股骨头粗隆相嵌插,增加骨折端的接触面积.结果随访6个月至4年,平均41个月.骨折愈合良好33例(91.7%),不愈合1例(2.8%),股骨头缺血坏死2例(5.6%).关节功能恢复情况按Harris评分标准优19例,良13例,可3例,差1例,优良率为88.9%.结论骨折远端截骨内固定提高股骨颈头下、头颈型骨折骨折愈合率以及降低股骨头坏死率.     

太康县1996～2000年AFP病例监测情况的分析

<正>快速、敏感、完整系统的AFP监测工作是消灭脊髓灰质炎(以下简称“脊灰”)的重要策略之一,它为实现2000年消灭脊灰提供了可靠的科学依据。因此,为总结经验教训,进一步提高监测报告的质量,确保太康县消灭脊灰证实后保持无脊灰工作的持续性开展,正确的指导今后的AFP监测工作,现对太康县1996～2000年AFP病例监测情况作如下分析:     

心肌营养素-1的研究进展

心肌营养素-1（cardiotrophin-1，CT-1）最初是Permica等于1995年从小鼠胚胎干细胞的胚状体培养液中分离获得的一种新型细胞因子，其结构与细胞因子IL-6家族成员如白血病抑制因子（leukamia inhibitory factor．LIF）、睫状神经营养因子（ciliary neurotrophic factor．CNTF）抑瘤素M（oncostatin M．OSM）与白细胞介素-11（Interleukin-11,IL-11）具有高度同源性，属IL-6家族成员。研究表明，CT-1作为一种多效性细胞因子，不仅能够诱导新生鼠心肌肥大，还对心肌和神经元的生存起重要保护作用。     

心肌营养素1修饰的神经干细胞移植对癫(癎)持续状态大鼠海马损伤及苔藓纤维发芽的影响

Objective To observe the survival,migration and differentiation of grafted neural stem cells(NSCs)transfected with cardiotrophin-1(CT1)in hippocampus in status epilepticus(SE)rats,and investigate its effect on neuron loss and mossy fiber sprouting(MFS)in hippocampus of SE rats.Methods (1)Lithium-pilocarpine induced SE model rats were divided into 3 groups randomly:CT1-NSCs transplantation group(n=18);NScs transplantation group(n=18)and SE model group(n=18).Another 18 rats served as normal control group.Each group was further divided into 3 time points testing groups(n=6 at each point)corresponding to 1,4 and 8 weeks after transplantation respectively.(2)Under the confocal microscopy,the survival,migration and differentiation of the grafted cells were observed by immunofluorescenee.(3)Morphological changes and neuron loss in the hippocampal CA1 region were examined by Nissl staining.(4)MFS in hippocampal dentate gyrus in rats was obserred by Timm histochemistry.Results(1)At 4 and 8 weeks post-tmusplantation,the numbers of double-labeled NF-200 and EGFP pesitive cells in the CT1-NSCs group were significantly hisher than those in NSCs group.In the former group most of the grafted NSCs migrated away from the needle tract,but in the latter group,grafted ceHs remained at the transplantation site.(2)The numbers of neuron in the hippocampal CA1 region reduced gradually after SE.The numbers of neuron in the CA1 region in CT1-NSCs transplantation rats (68.85±11.49,60.89±12.17 and 51.51±13.34 in 1,4,8 weeks after transplantation respectivelv)were greater than that in NSCs transplantation rats(67.92±10.78,42.56±11.47 and 30.49±10.12).tvalue were 4.650 and 5.334 in 4 and 8 weeks after transplantation(P<0.05).(3)Aberrant MFS in the inner molecular layer of dentate gyrus was observed,and the scores of MFS gradually increased with timelapse.The scores of MFS in CT1-NSCs transplantation rats(0.77±0.04,2.48±0.89 and 2.39±0.82 in 1,4,8 weeks after transplantation respectively)were significant lower than that in NSCs transplantation rats (1.12±0.62,3.17±0.64 and 3.88±0.51,t=6.059,9.511 and 9.728,P<0.05).Conclusions CT1 could promote the survival,migration and differentiation of engrafted NSCs in hippocampud in SE rats.Engrafted NSCs transfected with CT1 have effect on repair of the injured hippocampus,and could inhibit hippocampus MFS in SE rats.     

心肌营养素1修饰的神经干细胞移植对癫(癎)持续状态大鼠海马损伤及苔藓纤维发芽的影响

Objective To observe the survival,migration and differentiation of grafted neural stem cells(NSCs)transfected with cardiotrophin-1(CT1)in hippocampus in status epilepticus(SE)rats,and investigate its effect on neuron loss and mossy fiber sprouting(MFS)in hippocampus of SE rats.Methods (1)Lithium-pilocarpine induced SE model rats were divided into 3 groups randomly:CT1-NSCs transplantation group(n=18);NScs transplantation group(n=18)and SE model group(n=18).Another 18 rats served as normal control group.Each group was further divided into 3 time points testing groups(n=6 at each point)corresponding to 1,4 and 8 weeks after transplantation respectively.(2)Under the confocal microscopy,the survival,migration and differentiation of the grafted cells were observed by immunofluorescenee.(3)Morphological changes and neuron loss in the hippocampal CA1 region were examined by Nissl staining.(4)MFS in hippocampal dentate gyrus in rats was obserred by Timm histochemistry.Results(1)At 4 and 8 weeks post-tmusplantation,the numbers of double-labeled NF-200 and EGFP pesitive cells in the CT1-NSCs group were significantly hisher than those in NSCs group.In the former group most of the grafted NSCs migrated away from the needle tract,but in the latter group,grafted ceHs remained at the transplantation site.(2)The numbers of neuron in the hippocampal CA1 region reduced gradually after SE.The numbers of neuron in the CA1 region in CT1-NSCs transplantation rats (68.85±11.49,60.89±12.17 and 51.51±13.34 in 1,4,8 weeks after transplantation respectivelv)were greater than that in NSCs transplantation rats(67.92±10.78,42.56±11.47 and 30.49±10.12).tvalue were 4.650 and 5.334 in 4 and 8 weeks after transplantation(P<0.05).(3)Aberrant MFS in the inner molecular layer of dentate gyrus was observed,and the scores of MFS gradually increased with timelapse.The scores of MFS in CT1-NSCs transplantation rats(0.77±0.04,2.48±0.89 and 2.39±0.82 in 1,4,8 weeks after transplantation respectively)were significant lower than that in NSCs transplantation rats (1.12±0.62,3.17±0.64 and 3.88±0.51,t=6.059,9.511 and 9.728,P<0.05).Conclusions CT1 could promote the survival,migration and differentiation of engrafted NSCs in hippocampud in SE rats.Engrafted NSCs transfected with CT1 have effect on repair of the injured hippocampus,and could inhibit hippocampus MFS in SE rats.     

一期前路病灶清除后路内固定治疗胸腰段结核合并巨大脓肿

目的 探讨胸腰段椎体结核合并有巨大寒性脓肿行一期前路病灶清除,椎管减压,后路内固定植骨融合的疗效.方法对1999-01～2005-12间收治的22例胸腰椎结核合并有巨大寒性脓病例,采用同期前路病灶清除,后路椎弓根螺钉内固定,自体植骨融合治疗.结果术后随访6～24个月,平均9个月,22例脊柱结核患者均治愈,症状消失,无局部结核复发,植骨全部骨性融合,融合时间为5～7个月,后凸矫正率达80%.结论在胸腰椎结核手术中,后路内固定和前路病灶清除与植骨可同期进行,能有效地稳定病椎,部分矫正畸形,消灭病灶,缩短卧床时间.     

脊髓型颈椎病前路手术后并发食管憩室瘘1例

1 病例资料 患者,男,38岁.因四肢麻木2年,加重伴双下肢行走不稳1年,于2006年3月8日入院.专科检查:双手、双足痛温觉减退,双手握力3级,肱二、肱三头肌力3级,双下肢肌力4级,双侧Hoffmann征( ),四肢腱反射亢进,Babinski征(-),颈椎MRI示C5～6椎间盘突出伴脊髓受压.诊断为脊髓型颈椎病.3月12日,在颈丛 局麻下前路行C5～6椎间盘切除,自体髂骨植骨钛板内固定术,术后予抗炎、止血、对症治疗.     

不稳定型骨盆骨折的手术治疗分析

目的探讨切开复位内固定治疗各类不稳定骨盆骨折的术式选择及疗效.方法对37例不稳定型骨盆骨折手术病人,根据Tile分类选择手术及内固定方式:B型20例,C型17例,分别采取前方钢板螺钉固定和后方螺钉固定.结果 37例随访12～25个月,平均12.5个月,全部恢复行走功能,外形良好,X线平片显示:所有骨折均骨性愈合,根据Matte评定标准,优30例,良7例.结论切开复位内固定是治疗不稳定骨盆骨折的有效方法,选择合理的手术及内固定方式,可有效地防止畸形发生,并减少卧床时间,利于功能锻炼,从而取得良好的疗效.