高效液相色谱法测定米非司酮胶囊相对生物利用度

目的建立人血浆中米非司酮浓度的HPLC测定方法,研究健康受试者口服米非司酮胶囊的药动学,以国产同品种片剂作为参比制剂,计算两制剂的相对生物利用度,判断两种制剂是否等效.方法18名受试者随机分为两组,先后单剂量口服受试制剂和参比制剂后,采用HPLC法测定血药浓度,并计算药动学参数,经方差分析和双单侧t检验进行等效性判断.结果米非司酮最小检测浓度为0.075 mg·L-1,回收率大于85%,日内和日间RSD均≤5%.主要药动学参数分别为米非司酮胶囊tmax为(1.03±0.27)h,AUC0-48为(5 048.7±552.2)μg·h·L-1,Cmax为(461.6±38.5)μg·L-1,t1/2为(44.6±13.5)h.参比制剂tmax为(0.94±0.16)h,AUC0-48为(5 085.8±544.5)μg·h·L-1,Cmax为(4 68.6±51.8)μg·L-1,t1/2为(40.7±9.3)h.结论建立的HPLC法简便快速,定量可靠准确,适合于米非司酮胶囊临床研究.米非司酮胶囊的相对生物利用度为(99.8±10.5)%,经统计学分析,两制剂生物等效.     

锌治疗小儿厌食

佝偻病患儿39例,占26％;营养性贫血37例,占24.6％;反复呼吸道感染24例,占16％;异食癖8例,占5.3％;身材明显矮小2例,占1.3％;健康儿童40例,占26.6％。对患有原发病的儿童均进行了对症治疗,但厌食不见好转。对本组儿童均给予锌剂治疗:以0.2％硫酸锌糖浆口服,     

去甲肾上腺素和前列腺素F_(2α)对家兔黄体的相互作用

对家兔在体卵巢灌流条件下加入去甲肾上腺素(NA)与前列腺素F_(2α)(PGF_(2α))对人绒毛膜促性腺激素(HCG)、促进黄体合成孕酮(P)及环磷酸腺苷(cAMP)的功能有无影响进行了研究。结果表明,HCG能刺激兔早期黄体cAMP和P的产生;PGF_(2α)不能对抗HCG的这种作用,但能明显拮抗HCG促进兔中期黄体cAMP和P产生的作用。兔早期黄体灌流液中加入NA,则PGF_(2α)又能明显抑制HCG促进cAMP和P产生的作用。提示NA对家兔具有调节前列腺素溶黄体的作用。     

倍频Nd∶YAG/532激光在离体猪心肌打孔中工作参数的探讨

目的比较KTP-Nd∶YAG/532激光和Ho∶YAG激光对离体猪心肌打孔的组织学效应,探讨KTP-Nd∶YAG/532激光用于激光心肌血运重建术的可能性.方法用KTP-Nd∶YAG/532激光和Ho∶YAG激光分别以4种工作参数在离体猪心左心室心内膜面和心外膜面进行心肌打孔,光镜下测量激光隧道的直径、深度和热损伤层厚度,对两种激光心肌隧道的组织学变化进行比较.结果 KTP-Nd∶YAG/532激光和Ho∶YAG激光以4、6、8次触发均能形成有效激光隧道,随触发次数的增加,激光隧道深度增加.Ho∶YAG激光形成的隧道不规则,热损伤层大,KTP-Nd∶YAG/532激光形成的隧道顺直、周围热损伤小.结论 KTP-Nd∶YAG/532激光能形成有效心肌激光隧道,具有隧道顺直,周围组织热损伤小的优点,是激光心肌血运重建术(TLMR)可供选择的激光源.     

多媒体技术在临床医学教育中的应用探讨

随着计算机技术的日新月异,多媒体技术已广泛地应用于各种教学领域中。21世纪多媒体技术更是广泛地应用于高等医学院校。当前,高等医学教育改革中一个最热门的话题就是关于计算机辅助教学系统的应用,即如何合理的将多媒体技术引入课堂教学中。多媒体辅助教学既是对传统教学方法     

Stability of human follicle-stimulating hormone receptor mRNA in stably transfected cells

Follicle- stimulating hormone( FSH) is essentialfor ovarian follicular development in the female andenhances spermatogenesisin themale. FSH exerts itsactions via specific,high affinity membrane- boundreceptors( FSHR) that are coupled to guanine nu-cleotide binding proteins and stimulates the enzymeadenylyl cyclase[1] . The isolation,cloning and se-quencing of FSHR c DNAs from several species[2 ]have facilitated analysis of FSHR m RNA expressionin the ovary and testis. m RNA contentfr…     

P38αMAPK在雌性生殖系统中的研究进展

丝裂原活化蛋白激酶（mitogen-activated protein kinases,MAPK）是真核生物长期进化过程中高度保守的丝氨酸/苏氨酸蛋白激酶,MAPK在许多生物中发挥着重要作用,包括细胞生长、分化、凋亡、炎症反应、细胞骨架重排及应激刺激等。P38MAPK是其中重要的一个亚家族,其中P38α是P38MAPK中最重要的亚型,在雌性生殖系统中有着重要的作用。卵巢在受到体内激素或者外界因素等信号刺激时,通过激活P38αMAPK信号通路,表达各种细胞调控因子以调控颗粒细胞活动及卵母细胞减数分裂,进而影响生殖细胞的生长发育。激活颗粒细胞中P38αMAPK信号通路可以调控激素分泌及相关细胞因子的释放,从而有助于卵母细胞的成熟;P38αMAPK可能参与了卵母细胞的减数分裂过程,下调P38α的表达,可能导致成熟卵母细胞数目减少或者非整倍体卵母细胞的产生。     

大鼠脊髓不同平面损伤后神经源性膀胱模型的尿流动力学检查

目的观察大鼠不同平面脊髓损伤（SCI）后尿流动力学的改变，探讨不同平面SCI引起神经源性膀胱的病理生理机制。方法选择健康雌性SD大鼠80只，分为骶上脊髓（胸腰段）损伤组（30只）、骶髓损伤组（30只）及对照组（20只）。采用脊髓横断法制备大鼠脊髓不同平面损伤后神经源性膀胱模型。分别于动物模型制作前及制作后第20天行尿流动力学检查，评价大鼠的膀胱功能。结果骶上脊髓损伤组逼尿肌漏尿点压较对照组、骶髓损伤组明显升高，差异有统计学意义（P〈0．01），骶髓损伤组逼尿肌漏尿点压较对照组显著降低，差异有统计学意义（P〈0．01）；骶上脊髓损伤组膀胱最大容量、膀胱顺应性较对照组、骶髓损伤组明显降低，差异有统计学意义（P〈0．01），骶髓损伤组膀胱最大容量、膀胱顺应性较对照组显著增加，差异有统计学意义（P〈0．01）。结论尿流动力学检查为神经源性膀胱的早期诊断及选择个体化治疗方案提供了一种有效的检查手段。     

急性心肌梗死患者应用激光心肌血运重建术后循环和心脏局部肾素-血管紧张素系统的变化

激光心肌血运重建术 (TMLR)是近年来治疗晚期缺血性心脏病的一种新方法 ,可以单独应用或作为冠状动脉搭桥术(CABG)的一种辅助治疗手段 ,能改善心绞痛症状及提高生活质量。但其对长期心脏功能的影响及远期预后却有不同的结论 ,多数临床研究认为TMLR不能改善长期心脏功能及远期预     

Effects of Antisense Oligodeoxynucleotide to Follicle-stimulating Hormone Receptor on the Cell Proliferation and Apoptosis in Cells Derived from Human Ovarian Mucinous Cystadenocarcinoma in Vitro

The human ovarian mucinous cystadenocarcinoma (hOMC) cells were co-cultured with antisense oligodeoxynucleotide (antisense ODN), nonsense ODN, and follicle-stimulating hormone (FSH) at different concentrations for the purpose of observing the effects of antisense ODN to FSH receptor (FSHR) on the proliferation and apoptosis of cultured hOMC cells in vitro. The inhibitory rates of growth were measured by using MTT method on the 2nd, 4th, 6th, 8th and 10th days after the interference of antisense ODN, nonsense ODN, and FSH, respectively. The apoptotic rates and the cell cycles were determined by means of flow cytometry, the apoptosis indexes were detected by us-ing TUNEL, and the expression of caspase-3 was measured by using SP immunohistochemistry. Compared with that in the control group, the proliferative activity of hOMC cells was increased ob-viously in FSH groups (P<0.05 or P<0.01), decreased distinctly in antisense ODN groups (P<0.05 or P<0.01), and unchanged in nonsense ODN groups, respectively. Meanwhile, antisense ODN could significantly antagonize the FSH-promoted cell proliferative activity (P<0.01). Compared with those in the control group, the apoptotic rates and the expression of caspase-3 were dramatically increased in the mid- and high-dose antisense ODN groups (P<0.05 or P<0.01), while the number of cells in G1/G0 phase was significantly decreased and that in S phase distinctly increased (P<0.01). There was no change in nonsense ODN groups (P>0.05). It was suggested that FSH may improve the develop-ment of hOMC cells. However, antisense ODN could inhibit proliferative activity and the FSH-promoted proliferative activity in hOMC cells, at the same time, antisense ODN could inhibit hOMC cell growth by inducing apoptosis.