齿科桩钉用纤维/树脂复合材料细胞毒性实验研究

目的：研究齿科桩钉用纳米SiO2／S－Gf/EAM复合材料的细胞毒性。方法：用四唑盐比色法研究纳米SiO2／S－Gf/EAM复合材料的细胞毒性。结果：随着培养时间的增加，所测材料各浓度组的吸光值也相应增加，阳性对照组增加较慢，空白对照组和测试材料组增加较快，低浓度组的吸光值高于高浓度组，实验材料的细胞毒性为0级。结论：纳米SiO2／S－Gf/EAM复合材料无明显细胞毒性。     

胆道感染和抗生素的选用

为分析当前胆汁中菌群分布及其药物敏感情况，探讨胆道感染的原因和特点，本文总结了７６例胆道感染病人术中胆汁需氧菌群及药物敏感情况。现在报告如下。资料与方法１．一般资料：本组７６例，男性３４例，女性４２例；年龄２２～７８岁，平均年龄５６．５岁。经手术证实伴发肝内胆管结石１１例（１４．４７％），胆总管结石３９例（５１．３２％），肝内外胆管结石２２例（２８．９５％），肝脓肿５例（６．５８％），阻塞性黄疸５例（６．５８％），急性胰腺炎８例（１０．５３％），先天性胆总管囊性扩张２例（２．６３％），胆道术后７…     

交锁髓内钉治疗胫腓骨骨折并发症防治

目的：观察交锁髓内钉治疗胫腓骨骨折的并发症及防治。方法：1997年2月-2000年6月用交锁髓内钉治疗胫腓骨骨折46例。男36例。女10例。新鲜闭合性骨折27例，开放性骨折15例。陈旧性骨折4例。左侧24例，右侧22例。年龄25-65岁，平均37岁，均采用扩髓及静力型固定。结果：46例均得到随访，平均20个月（6个月-3年）。愈合时间为3-12个月，平均4个月，感染4例均为开放性骨折，肢体短缩1例。成角畸形2例。延迟愈合7例。结论：交锁髓内钉治疗胫腓骨骨折的一些并发症存在必须引起重视。开放性骨折以不扩髓为宜。     

早期负压封闭引流治疗深Ⅱ度烧伤创面的临床疗效评价

Objective To evaluate the clinical curative effect of applying vaccum sealing drainage (VSD) therapy in treating deep partial-thickness burn wound at the initial stage prospectively, and to provide the basis for its clinical application. Methods Twenty-two patients with about 10% TBSA burn of the lower limbs, and in which partial-thickness wound exceeded 1% TBSA in each limb, were admitted to our hospital within 3 hours after burn from May 2009 to March 2010. Wounds in each patient were divided into VSD treatment group (treated with VSD therapy) and control group (treated with 10 g/L silver sulfadia-zine cream) based on the principles of symmetry of location, identical deepness, and similarity in size etc. The amount of water evaporation, the swelling intensity, the status of bacterial colonization, the degree of pain, the healing time, and the quality of healing of wounds in 2 groups were observed and compared. Data were processed with t test and rank-sum test. Results The observation was completed in 21 patients. All of the wounds were treated within 4 hours post burn (PBH). The amount of water evaporation of the normal skin and burn wounds before dressing coverage in VSD treatment group was respectively close to that in control group (with t value respectively 1.310, - 0. 911, P values all above 0.05) ; the amount of water evaporation on the surface of dressing in VSD treatment group [(44. 3 ±3.9) mL·h-1·m-2] was less than that in control group [(66.1 ±6.4) mL · h-1· m-2, t = -11.39, P <0.01]. In VSD treatment group, the circumference of proximal thigh increased (3.48 ±0.35) and (2.51 ±0.21) cm on post burn day (PBD) 3 , 7 as compared with that on PBH 5 , which was respectively smaller than that [(8.02 ± 0.41) , (3. 99 ± 0. 32) cm] in control group (with t value respectively 4. 110, 3. 569, P values all below 0. 01). Positive bacteria' culture rate on PBD 10 of each group was respectively lower than that at admission (with Z value respectively -3.220, -3.870, P values all below 0. 01) , and there was no significant statistical difference between 2 groups at admission or on PBD 10 (with Z value respectively - 0. 894, 0.000, P values all above 0.05). The wound surface in VSD treatment group was weak acidic (pH value 7. 12 ±0.06) on PBD 10,and it was neutral (pH value 7.41 ±0. 13) in control group. The wound pain degree in control group on PBD 1,3,7 was respectively higher than that in VSD treatment group (with t value respectively - 16. 132, -21.230, -16.453, P values all below 0.01). There was no significant statistical difference between 2 groups in healing time of wounds (t =1. 186, P >0.05). The healing quality of wounds in VSD treatment group (100. 00% , 100. 00%) 2 or 3 months after burn was better than that in control group (19. 05% , 85. 71%) (with Z value respectively -11.638, -3. 870, P values all below 0.01). Conclusions Early application of VSD therapy cannot expedite the healing process of deep partial-thickness burn wounds, but it can improve the healing quality. It is one of the effective methods to deal with deep partial-thickness burn wounds, which is worthy of clinical attention and further research.     

埃罗替尼和塞来昔布协同抑制胆管癌细胞生长

目的:观察表皮生长因子受体(EGFR)酪氨酸激酶抑制剂埃罗替尼(erlotinib)与环氧合酶(COX-2)抑制剂塞来昔布(celecoxib)对胆管癌细胞生长的协同抑制作用。方法:采用甲基噻唑基四唑(MTT)比色法检测胆管癌细胞株QBC939增殖,流式细胞术检测用药前、后细胞凋亡和细胞周期的变化,逆转录聚合酶链反应(RT-PCR)、Western印迹方法观察用药前、后细胞中COX-2、EGFR下游信号及凋亡、细胞周期相关基因蛋白表达的变化。结果:QBC939细胞表达COX-2 mRNA和EGFR mRNA及相应蛋白。埃罗替尼、塞来昔布抑制QBC939细胞增殖,促进细胞凋亡,诱导细胞周期阻滞于G0/G1期。同时,塞来昔布增强了埃罗替尼抑制细胞生长、诱导细胞凋亡的作用。联合用药显著降低p-MAPK、p-Akt及PGE2表达。结论:塞来昔布和埃罗替尼均可抑制胆管癌细胞的生长,而联合用药具有协同作用,能同时阻断EGFR和COX-2信号通路。在胆管癌治疗中具有一定应用前景。     

胰岛素泵和甘精胰岛素在2型糖尿病患者围手术期的疗效及费用的比较

目的:比较胰岛紊泵和甘精胰岛素在2型糖尿病(T2DM)患者围手术期控制血糖选标时间及低血糖的发生率及费用的差别.方法:将80例围手术期T2DM患者分为胰岛素泵治疗组(CSII组)42例,甘精胰岛素治疗组(G组)38例.比较各组患者血糖达标时间及低血糖的发生率及费用的差别.结果:CSII组和G组在血糖达标时间及低血糖发生率上无明显差别,而G组在费用方面明显低于CSII组.结论:胰岛素泵及甘精胰岛素在T2DM患者围手术期均能安全、有效、快速、平稳降低血糖,且甘精胰岛素费用较低.     

妊娠期妇女甲型H1N1流感15例临床分析

甲型H1N1流感是一种由新型流感病毒感染所致的急性呼吸道传染病,传染性强,病情进展迅速.妊娠妇女作为甲型H1N1流感重症病例的高危人群,更应引起重视.为尽快了解其相关特性,总结防治经验,笔者对温州市第二人民医院收治的15例甲型H1N1流感妊娠期妇女进行了分析.     

单侧唇裂鼻畸形美学修复临床研究(265例报告)

目的:探讨对单侧唇裂畸形中鼻畸形的修复和方法选择.方法:在265例单侧唇裂进行鼻翼畸形修复,根据不同情况进行鼻翼弧形切口、飞鸟状鼻小柱、鼻翼联合切口、患侧鼻翼穹隆部新月形皮肤切除等方法综合治疗.结果:单侧唇裂的裂度与鼻畸形的程度成正比,修复方法较为复杂和多样术式.结论:唇裂鼻畸形修复采用美学的分析对鼻翼软骨进行对称性设计,达到美学效果.     

索拉非尼抑制肝癌细胞增殖中自噬的作用及其机制

目的：研究分子靶向药物索拉非尼在体外对人肝癌细胞株HepG_2增殖抑制过程中自噬的表达及作用,并探讨其可能机制。方法：以吖啶橙染色荧光显微镜对自噬进行定性观察;cell counting kit-8检测活性氧（reactiveoxygen species,ROS）抑制前后HepG_2细胞成活率的变化;RT-PCR检测自噬基因Beclin-1表达的变化。Western印迹检测自噬相关蛋白Beclin-1的变化：荧光分光光度计检测胞内二氯荧光素DCF的荧光强度。结果：索拉非尼对肝癌细胞HepG_2具有显著的抑制作用;索拉非尼可诱导肝癌细胞HepG_2产生自噬及ROS,自噬在基因及蛋白水平表达均增加;抑制ROS的产生可减少索拉非尼诱导的肝癌细胞HepG_2自噬的表达量,自噬的抑制增强了索拉非尼对肝癌细胞的抑制作用。结论：ROS参与索拉非尼诱导肝癌细胞HepG_2的自噬表达,索拉非尼在抑制肝癌细胞自噬过程中自噬可能起到保护作用,抑制自噬可能为提高进展期肝癌病人索拉非尼分子靶向治疗敏感性提供新的思路。