磷酸二酯酶抑制剂己酮可可碱对人精子受精力的影响

为进一步证明己酮可可碱(pentoxifylline,PF)对精子受精力的影响,对29例临床送检标本进行精液常规分析及体外受精实验.结果:6例精液常规及受精率均低于正常;14例精液常规正常, 但受精率低;9例精液常规及受精率均正常.所有标本均提取上游精子, 其中部分经体外获能后用作对照,部分用1mg/ml PF,37℃, 孵育0.5小时后再行体外获能.获能精子与去透明带金黄地鼠卵行异种体外受精,然后观察受精率,受精指数,及每个卵表面附着的精子平均数,结果提示PF对精液常规正常但受精力低下的精液标本有明显增强精子受精力的作用,这类患者精子经PF体外处理可能提高人工助孕技术的成功率.     

小鼠附睾分段采集的精子穿透实验设计与实践

改进原精子穿透实验(Sperm penetration assay,SPA)教学方案,以适应现实需求.采用分段采集的小鼠附睾精子取代人精子标本完成SPA,引入对不同成熟度精子受精能力的差异及钙通道抑制剂硝苯地平影响的观察,实验更安全、环保,压缩实验时长和成本的同时还丰富了实验内容、提升学生兴趣.     

人精子表面整合素亚基α5、β1参与受精过程的研究

目的：研究人精子表面整合素亚基α5、β1在受精过程中的作用。方法：以人精子穿透去透明带金黄地鼠卵异种体外受精试验（SPA）作为检测人精子受精力的手段；分别用抗整合素亚基α5、β1抗体衣整合素特异的配体RGD肽作用于精子后，观察其受精力的改变。结果：用抗整合素亚基α5、β1抗体及RGD肽作用于精子后，受精率、受精指数及粘附指数均下降。结论：人精子表面整合素亚基α5、β1参与受精过程。     

The Clinical Analysis of Endogenous Endophthalmitis

Endogenousendophthalmitisisavitalocularemergencybeingsubjectedtomisdiagnosisduetoitslowincidence.Therefore,theendoge鄄nousendophthalmitishaspoortherapeuticeffic鄄acyandispronetoblindness,evenotherseverecomplicationssuchasorbitalcellulitis,eyeballperforation,sepsisandsoon.TherehavebeenonlycasereportsofthisentityinChina.Tounderstandthisdiseasebetter,twenty鄄eightcon鄄secutivecases(28eyes)inourhospitalfrom1998toMarch2003.weresurveyedretrospectively.MethodsGeneralmaterialsThereweretotallytwenty…     

陕西省新型冠状病毒感染的肺炎中医药治疗方案(试行第二版)

新型冠状病毒肺炎是2019年12月自湖北省武汉市聚集并爆发的一种新型呼吸道传染病,根据该疾病为新发传染病、传变迅速且以发热、干咳、乏力、脘痞为症状特征以及舌质淡红苔白或薄黄腻、脉濡的特点,结合陕西省今年冬季以来没有有效降雪的气候实际,中医对该病命名为"风瘟"病,病机特点为"寒、湿、热、毒",病期可分为轻症、重症、恢复期,证型以九型论治。参考国家中医药管理局制订的诊疗指南,结合我省新型冠状病毒肺炎的发病特点及临床实践,撰写本诊疗方案,以提高临床疗效。     

Cyclin G1在小鼠卵巢的表达及其与卵泡发育的关系

目的观察细胞周期素G1（Cyclin G1）在小鼠卵巢中各级卵泡的表达及其与卵泡发育的关系。方法性成熟前小鼠用孕马血清促性腺激素（PMSG）刺激卵泡生长,用绒毛膜促性腺激素（hCG）刺激卵泡排卵及向黄体转化。于给药后不同时间取出卵巢,用免疫组化方法检测不同发育阶段卵泡Cyclin G1蛋白的表达和分布;用细胞免疫荧光方法检测不同成熟阶段的卵细胞中Cyclin G1蛋白表达。结果免疫组化染色结果显示,颗粒细胞中Cyclin G1的表达水平随卵泡发育成熟而逐渐增强,且一直定位在胞核;Cyclin G1在各个时期卵泡的卵母细胞中均有表达。该表达的定位与卵泡生长发育的不同阶段有关。随着卵泡的发育,Cyclin G1在卵母细胞的表达由胞核转至胞浆。至排卵前卵泡颗粒细胞,Cyclin G1表达水平减弱,当颗粒细胞分化为黄体细胞后表达水平进一步减弱。闭锁卵泡中Cyclin G1的表达水平很弱。细胞免疫荧光染色结果显示,处于生发泡时期（GV）卵母细胞仅有较弱的Cyclin G1表达,当卵母细胞生发泡破裂（GVBD）后,该表达增强;与处于第二次减数分裂中期（MⅡ）的卵母细胞相比,受精卵的Cyclin G1表达明显增强。结论Cyclin G1在小鼠卵泡发育及卵母细胞成熟过程中的表达具有时空特异性,可能作为细胞周期的正调节因子参与了卵泡生长发育和卵母细胞成熟过程的调控。     

Immediate reconstruction following resection of oral cancer with free rectus abdominis myocutaneous flap

目的：研究游离腹直肌肌皮瓣即刻修复口腔颌面部恶性肿瘤切除术后缺损的方法并评价其作用。方法：６例患者在进行广泛的口腔癌切除术后,应用游离腹直肌肌皮瓣进行缺损即刻修复,３例颊癌患者分别切除唇,颊,下颌骨及上颌骨后造成大面积洞穿性缺损,２例舌癌及１例下颌骨恶性肿瘤患者在进行舌切除及下颌骨切除后造成大面积及复杂的缺损,缺损修复的转移皮瓣最大面积达１１０ｍｍ×２３０ｍｍ。结果：游离腹直肌肌皮瓣及供区无严重的手术并发症,６例游离腹直肌肌皮瓣有５例愈合无并发症,１例皮瓣出现部分坏死,供皮区腹壁无组织感染及裂开。结论：游离腹直肌肌皮瓣使口腔颌面部缺损修复在功能和美观上达到满意的效果,提高了口腔颌面部恶性肿瘤患者广泛切除术后的生存质量。     

Cyclin G1在小鼠卵巢颗粒细胞的表达及调控

目的:探讨cyclinG1在小鼠卵巢颗粒细胞的表达以及促性腺激素对其表达的调控作用。方法:以免疫组化方法检测小鼠卵巢颗粒细胞中cyclinG1的表达情况;取性成熟小鼠的颗粒细胞进行体外培养,分别加入卵泡刺激素(FSH)和绒毛膜促性腺激素(hCG)作用12h后,收集细胞,提取细胞总RNA,采用半定量RTPCR检测cyclinG1表达水平的变化。结果:免疫组化结果显示cyclinG1在不同发育阶段卵泡的颗粒细胞和卵丘细胞中均有较强表达,而黄体细胞中表达较少。RTPCR结果显示,FSH与hCG分别作用于体外培养的颗粒细胞12h后,与对照组比较,hCG可使cyclinG1的表达水平明显降低(P<0.01),FSH对其表达水平影响不明显(P>0.05)。结论:小鼠卵巢颗粒细胞中存在cyclinG1表达;hCG可降低cyclinG1在颗粒细胞中的表达水平,提示cyclinG1在颗粒细胞增殖分化过程中可能发挥正调节作用。     

Methods of testing feasibility for sequenced treatment alternatives to relieve depression (STAR*D)

In large multi-site trials, a feasibility or pilot study can be crucial to test the functionality of all aspects of conducting the study prior to the initiation of the formal study. A feasibility trial was conducted for the Sequenced Treatment Alternatives to Relieve Depression (STAR*D) Project, a multi-site, prospective, sequentially randomized, clinical trial of outpatients with nonpsychotic major depressive disorder. From 14 December 2000 to 8 June 2001, 42 patients were screened for enrollment into the STAR*D Feasibility Trial. Twenty-four patients who were eligible and consented to participate were treated with citalopram for up to 12 weeks. During the course of this trial, issues were raised that resulted in modifications to the study procedures. Modifications made as a result of this trial affected four domains: (1) communication, (2) patient and provider burden, (3) data collection forms, and (4) recruitment and retention of subjects. This paper describes what was learned during the STAR*D Feasibility Trial so researchers planning to conduct similar trials can learn the practical issues related to conducting such a research project. While the information gathered was useful, it did delay the initiation of the formal trial. We view this cost as an investment in the development of overall study procedures that should lead to a stronger study.