针灸脑缺血再灌注损伤大鼠百会及足三里穴位对热休克蛋白70及肿瘤坏死因子-α表达的作用

Objective:To explore the effects of acupuncture on the peripheral serum expression of heat shock protein 70(HSP70)and tumor necrosis factor a(TNF-α)in rats with cerebral ischemia-reperfusion injury(CIRI).Methods:In total,152 Sprague-Dawley(SD)rats were randomly divided into an operated group and a non-operated group according to a random digits table.The operated group included a sham-operated group,a model group and an acupuncture group,whereas the non-operated group consisted of a normal group.Except for the normal group,each group was further divided into 12,24,48,72,96,and 144 h time points according to different reperfusion times.Eight rats were assigned in each operated group and in the normal group.The rat model of CIRI was established by the thread occlusion method in the model and acupuncture groups.The acupuncture group was treated with electroacupuncture at Baihui(DU20)and Zusanli(ST36)for the required time after successful operation.Blood was sampled to detect the HSP70 and TNF-αcontent by enzyme linked immunosorbent assay.Results:The expression of HSP70 protein in the peripheral serum of the experimental groups was higher than that in the normal control group.The peak time in both the model and the sham-operated groups was 12 h,and the peak time in the acupuncture group was 24 h.The expression in the acupuncture group declined to a lower level at 72 h and was lower than that in the model and sham-operated groups(P0.05).The peak time for the expression of TNF-αprotein in the peripheral serum of both the model and the acupuncture groups was 24 h,but the expression in the acupuncture group was lower than the model group.Additionally,the expression of TNF-αin all experimental groups was higher than the normal group(P0.05).Conclusions:Acupuncture at DU20 and ST36 in rats attenuated CIRI,which was associated with a reduction in the expression of HSP70 and TNF-α.These results provide clues to acupunctural neuroprotective properties.Acupuncture at DU20 and ST36 in rats after CIRI can adjust the expression of HSP70 and TNF-αin the peripheral serum,which might be one of the mechanisms of acupuncture's attenuation of CIRI.     

简易血管腔内电凝闭合术治疗大隐静脉曲张33例

大隐静脉曲张是血管外科常见疾病,我国人群中约25%的女性和15%的男性罹患此病[1]。传统的大隐静脉高位结扎加剥脱术疗效肯定,但创伤大、恢复慢且遗留明显手术瘢痕。近年来,随着微创技术的发展和治疗理念的进步,各种微创术式应运而生。激光静脉腔内闭合术(EVLT)治疗大隐静脉曲张发展迅     

ISSR技术在药用植物种质研究中的应用

介绍了ISSR技术的原理和特点,综述了ISSR技术在药用植物的种质资源鉴定、遗传多样性和遗传结构、分子辅助育种等方面的研究进展。     

针刺对脑缺血再灌注损伤大鼠外周血清IL-1β、IL-6表达的影响

目的:探讨针刺百会和足三里穴对脑缺血再灌注损伤大鼠外周血清不同时间点白细胞介素1β(IL-1β)、白细胞介素6(IL-6)表达的干预作用。方法:将SD大鼠随机分为假手术组、模型组和针刺组3个大组,每大组再按再灌注后时间分为12 h、24 h、48 h、72 h、96 h和144 h等6个亚组,每亚组8只大鼠;另设正常对照组8只。以大脑中动脉线栓法制备大鼠脑缺血再灌注模型,用酶联免疫吸附测定法测各组大鼠外周血清IL-1β、IL-6的表达。结果:IL-1β在针刺组表达强度低于模型组,以24 h组和96 h组最显著(P<0.05);IL-6在针刺组的表达低于模型组和假手术组,其中24 h和48 h组具有显著性差异(P<0.05)。结论:针刺百会和足三里穴可抑制脑缺血损伤早期炎症反应,延缓组织损伤。     

代赭石炮制的探讨

代赭石应用历史悠久。但代赭石生用还是煅用,从古到今无一定论。为此,作者对代赭石炮制前后含砷量的变化进行了探讨。结果表明,煅代赭石中砷的含量大大减少,并且煅后代赭石质地松脆,易于有效成分的溶出。故建议应将砷含量作为摄制代赭石的质量标准。     

500例住院儿童眼外伤统计分析

在我院1957年～1979年22年住院病例中,15岁以下的儿童眼外伤共500例,分析如下: 一、我院22年中住院眼外伤共3,659例,其中儿童有500例,占13.6%。     

中药轻粉的制造及其化学原理

轻粉是中医外科常用药,它有止痒、拔毒、去腐生肌的功能。常用以治疗疥癣、湿疹、瘰疬、阴疮等外科疾患。此外还可适量内服,具有祛痰、缓泻、利尿、杀虫、消积的功能,治梅毒等疾病均有相当疗效。轻粉的炼制,我国最早的文献记载是唐·     

关节镜的正确使用和维护保养

主要介绍关节镜的结构;如何正确使用;以及术前、术后的灭菌消毒和关节镜各部件的维护保养。     

外伤性眶内出血致眶尖综合征二例

眶尖综合征又称Ｒｏｌｅｔ综合征，是眼科少见病，多由外伤、肿瘤、炎性病变累及眶尖部引起。现将收治的２例外伤性眶内出血致眶尖综合征的治疗体会报告如下。例１男，２６岁。入院前一天骑车与他人相撞伤及右眼，自觉右眼胀痛、视物不清，ＣＴ诊断为眶内出血而收入院。查...     

Effects of Acupuncture at Baihui(GV 20) and Zusanli(ST 36) on Peripheral Serum Expression of MicroRNA 124,Laminin and Integrin β1 in Rats with Cerebral Ischemia Reperfusion Injury

Objective:To explore the effects of acupuncture at Baihui(GV 20) and Zusanli(ST 36) on the peripheral serum expression of microRNA 124(miRNA 124),laminin and integrin β 1 in rats with cerebral ischemia reperfusion injury(CIRI).Methods:Seventy-two healthy male Sprague-Dawley rats were randomized into a model group,an acupuncture group,and a sham-operated group using a random digits table,with 24 rats per group.Each group was further randomly divided into 1-,3-,5-,and 7-day subgroups based on the reperfusion time according to a random digits table,with 6 rats in each subgroup.In the model and acupuncture groups,CIRI was induced using the thread occlusion method.Electroacupuncture stimulation was applied daily to GV 20 and left ST 36 for 20 min at the indicated time points after successful operations.Serum was sampled for detecting laminin and integrin β1 protein via enzyme-linked immunosorbent assay,and serum miRNA 124 was examined using quantitative polymerase chain reaction.Results:The serum level of miRNA 124 in the cerebral ischemia rats increased significantly,and the peak expression of miRNA 124 in both the model and acupuncture groups occurred at 3 days.The expression of miRNA 124 in the acupuncture group was higher than in the model group at the same time point(5.96 ±0.01 vs.3.11 ±0.04,P0.05).Laminin expression in serum from the cerebral ischemia group was higher than that in the sham-operated group.Compared with the model group,the level of laminin in the serum of the acupuncture group was significantly lower at each time point,especially at the 3-day,and 7-day time points(589.12±3.57 vs.793.05 ± 5.28,and 600.53 ±3.05 vs.899.06 ±5.74,P0.05).The level of integrin β 1 in the serum from the acupuncture group was lower than that in the model group particularly at the 3-day and 7-day time points(208.66 ±0.95 vs.280.83 ±1.77,and212.36 ±0.95 vs.316.77±2.42,P0.05).Additionally,the model group and the acupuncture group showed dual peaks of integrin β 1 and laminin expression at 3-day and 7-day.Conclusions:Acupuncture at GV 20 and ST 36 in rats alleviated CIRI and was associated with upregulated expression of miRNA 124 and with downregulated expression of integrin β 1 and laminin in peripheral serum.These changes may represent one of the mechanisms underlying acupuncture's attenuation of CIRI.