Osteogenic differentiation of bone mesenchymal stem cells regulated by osteoblasts under EMF exposure in a co-culture system

This study examined the osteogenic effect of electromagnetic fields （EMF） under the simulated in vivo conditions. Rat bone marrow mesenchymal stem cells （BMSCs） and rat osteoblasts were co-cultured and exposed to 50 Hz, 1.0 mT EMF for different terms. Unexposed single-cultured BMSCs and osteoblasts were set as controls. Cell proliferation features of single-cultured BMSCs and osteoblasts were studied by using a cell counting kit （CCK-8）. For the co-culture system, cells in each group were randomly chosen for alkaline phosphatase （ALP） staining on the day 7. When EMF exposure lasted for 14 days, dishes in each group were randomly chosen for total RNA extraction and von Kossa staining. The mRNA expression of osteogenic markers was detected by using real-time PCR. Our study showed that short-term EMF exposure （2 h/day） could obviously promote prolifera- tion of BMSCs and osteoblasts, while long-term EMF （8 h/day） could promote osteogenic differen- tiation significantly under co-cultured conditions. Under EMF exposure, osteogenesis-related mRNA expression changed obviously in co-cultured and single-cultured cells. It was noteworthy that most osteogenic indices in osteoblasts were increased markedly after co-culture except Bmp2, which was increased gradually when ceils were exposed to EMF. Compared to other indices, the expression of Bmp2 in BMSCs was increased sharply in both single-cultured and co-cultured groups when they were exposed to EMF. The mRNA expression of Bmp2 in BMSCs was approximately four times higher in 8-h EMF group than that in the unexposed group. Our results suggest that Bmp2-mediated cellular interaction induced by EMF exposure might play an important role in the osteogenic differ- entiation of BMSCs.     

安徽省淮河流域上游洪水型钩端螺旋体病流行因素的探讨

本文探讨了我省淮河上游洪水型钩体病流行区，自１９８１年该地人群停止钩体菌苗预防接种后，加强防治知识的宣传教育，传染源的管理，群众实施堆肥发酵，改变散放为圈猪积肥，猪的圈养率达８１１３％，减少猪之间相互感染，猪钩体带菌率由７０年代的８７６％降为１８９％〈４／２１２〉Ｐ＜０．０１；人群隐性感染率由２２３５％也降为１９４％Ｐ＜０．０１。改变猪散放为圈养的饲养方式，是行之有效的控制传染源的措施，从而控制本病的发生和流行     

科学灭鼠控制出血热发病

科学灭鼠控制出血热发病霍邱卫生防疫站（２３７４００）魏强刘明宋明宇许中安陈家新丁广修我县是安徽省流行性出血热发病稳定的老疫区，１９９１年因特大洪涝灾害，生态环境遭受严重破坏，鼠密度和出血热发病持续增高，据姜家湖乡临闸村监测，室内鼠密度达１０．０％，室...     

地塞米松对骨髓间充质细胞增殖及成骨、成脂分化的效应

目的 探讨不同浓度地塞米松(dexamethasone,DEX)作用不同时间对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)的增殖及成骨、成脂分化的影响.方法 体外分离培养大鼠BMSCs,将状态良好的第三代细胞接种于96孔板中,随机分组为对照组(不加入DEX)及不同浓度(1 nmol/L、10 nmol/L、0.1μmol/L、1μmol/L)DEX作用组,培养1、3、5、7、9 d后,分别用细胞增殖检测(CCK-8)法检测细胞增殖状况.将BMSCs接种于6孔板中,随机分为对照组及不同浓度(1 nmol/L、10 nmol/L、0.1μmol/L、1μmol/L)DEX作用组,培养7、14 d后分别用荧光定量PCR方法检测成骨、成脂相关基因的表达,培养14 d后用Western blot检测成骨、成脂相关蛋白的表达.培养5 d后进行碱性磷酸酶(alkaline phosphatase,ALP)染色,培养14 d后进行油红"O"染色、茜素红染色.结果 较低浓度的DEX促进BMSCs增殖,而较高浓度DEX抑制BMSCs增殖.干预早期,较低浓度的DEX促进BMSCs成骨分化而高浓度DEX抑制其成骨分化;干预晚期,各浓度DEX均促进BMSCs成骨指标的表达,但是不能诱导BMSCs钙质沉积.DEX浓度依赖性地促进BMSCs成脂相关指标基因和蛋白的表达,并诱导BMSCs细胞内脂质沉积.结论 DEX可直接影响BMSCs增殖及向成骨、成脂方向分化,这种效应存在浓度和干预时间依赖性.     

安徽省肾综合征出血热沙鼠肾细胞Ⅰ型灭活疫苗现场实施及防病效果观察

安徽省肾综合征出血热沙鼠肾细胞Ⅰ型灭活疫苗现场实施及防病效果观察宋明宇，魏强，陈家新，陈孝英，丁广修，孙仁品，许中安，花昌华，李含，周本良肾综合征出血热（ＨＦＲＳ）沙鼠肾细胞灭活Ⅰ型疫苗是新近研制的生物制品，经小范围人群接种试验证明是安全的。根据中国...     

安徽省肾综合征出血热沙鼠肾细胞Ⅰ型灭活疫苗免疫性及其流行病学效果的考核

采用ＥＬＩＳＡ、ＩＦＡ检测特异性抗体,ＭＣＰＥＮＴ检测中和抗体和ＨＩ,进行血清学分型。结果：选择姬鼠型疫区,接种沙鼠肾细胞Ⅰ型灭活出血热疫苗安全性较好。免疫后４２天ＨＦＲＳ．Ｖ．ＩｇＧ抗体阳性率为９６４３％,ＧＭＴ＝３５１８,１８０天有明显下降,加强免疫两周后阳性率达１００％,而且１２００天仍有３６３６％人群存在１∶１０（＋）抗体;ＭＣＰＥＮＴ抗体较ＩＦＡ抗体阳性率低,但疫苗免疫人群具有特异性回忆反应。中期流行病学防病效果接种组发病率为２３５／１０万,对照组为４２９３／１０万,保护率９４５３％。     

共培养条件下电磁场干预对大鼠成骨细胞及骨髓间充质干细胞成骨分化的影响

目的观察共培养条件下电磁场干预对大鼠成骨细胞及骨髓间充质干细胞(MSCs)成骨定向分化的影响,并探讨电磁场促进成骨分化的相关机制。 方法体外分离培养SD大鼠BMSCs及成骨细胞,将第三代成骨细胞与BMSCs通过transwell培养小室建立共培养系统。采用随机数字表法将共培养细胞分为共培养组及共培养暴磁组,另随机选择单细胞培养的BMSCs及成骨细胞纳入单细胞培养组。共培养暴磁组细胞每日给予电磁场刺激4 h。于实验进行14 d后随机提取各组细胞总RNA,采用荧光定量PCR技术检测各组细胞Runx2、Sp7、碱性磷酸酶、I型胶原、骨形态形成蛋白-2及骨钙素基因表达情况,并选用茜素红染色法检测各组细胞矿化钙结节形成情况。 结果单细胞培养模式下BMSCs及成骨细胞其各项成骨相关基因表达水平均较低,而共培养模式下BMSCs及成骨细胞其各项成骨相关基因表达均呈现不同程度增强,并且以共培养暴磁组成骨细胞上述指标增强幅度较显著。通过茜素红染色发现,与单纯共培养组比较,共培养暴磁组细胞矿化钙结节数量明显增多。 结论低频电磁场干预可显著促进共培养条件下BMSCs及成骨细胞成骨定向分化,其可能机制为电磁场刺激能促进骨形态形成蛋白-2表达,使其通过自分泌或旁分泌方式介导细胞间信号转导,从而诱导BMSCs向成骨细胞分化及成骨细胞进一步成熟分化。     

霍邱县猪携钩端螺旋体及健康人群钩体病隐性感染调查

霍邱县猪携钩端螺旋体及健康人群钩体病隐性感染调查顾莉莉１杭恒贵１王以银１任次早１罗兆庄１魏强２宋明宇２孙仁品２（１安徽省卫生防疫站２３００６１２霍印县卫生防疫症站）据７０年代调查，安徽省钩端螺旋体病（简称钩体病），在淮河流域地区，以洪水型为主，曾发生...     

霍邱县救灾防病工作对策与效果评价

1991年我县遭受了百年不遇的特大洪涝灾害,全县10个区镇,54个乡镇,626个行政村,29万户,121万人遭受特大洪涝灾害,直接经济损失13.5亿元。洪水围困期间,迁移到淮河大堤及沿岗地区的人畜禽共居又高度集中,水源污染严重,环境卫生恶劣,加上盛夏酷暑,