二氢青蒿素对胰腺癌生长的抑制作用

Objective To investigate the anti-tumor activity of dihydroartemisinin in pancreatic cancer in vitro and in vivo. Methods For cultured cells,cell growth was determined by the MTT assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITG/PI. The protein expression in BxPC-3 cells was analyzed by Western blot assay. BxPC-3 cells were injected subcutaneously into nude mice to establish pancreatic xenograft tumors and the tumor volume was monitored after exposure to dihydroartemisinin. Ki-67 staining and TUNEL assay were used to assess tumor cell proliferation and apoptosis in tumor tissue. Results After treatment by dihydroartemisinin in vitro, the proliferative inhibition rates of pancreatic cancer cells BxPC-3 and AsPC-1 reached up to (76.2 ± 3.5) % and (79.5 ± 2.9) %, and the apoptosis rates were up to (55.5 ± 3.2)% and (40.0 ± 3.5)%, the differences were significantly (P ＜ 0.01) compared with control [(2.0 ± 1.3) % and (0.9 ± 0.4) %]. Dihydroartemisinin inhibited the growth of pancreatic xenograft tumors in nude mice. The proliferation index and apoptusis index were (49.1 ± 3.9)% and (50.2 ± 4.4)% respectively in dihydroarternisinin 50 mg/kg treatment group, compared to those of (72.1 ± 3.3) % and (9.4 ± 2.9) % in control, the differences were significantly (P ＜0.01). Western blot assay indicated that dihydroartemisinin up-regulates expression of proliferation-associated protein p21WAF1 and down-regulates expression of PCNA, increases expression of apoptosis-associated protein Bax and decreases expression of Bcl-2 and activates caspase-9 in BxPC-3 cells. Conclusions Dihydroartemisinin exerts anti-tumor activity in pancreatic cancer both in vitro and in vivo by proliferation inhibition and apoptusis induction. Dihydroartemisinin can be used as a potential anti-tumor drug in pancreatic cancer.     

开设切片制作新实验,加强素质教育

教育部在高教[2001]4号文件《关于加强高等学校本科教学工作,提高教学质量的若干意见》中指出：“实践教学对于提高学生的综合素质,培养学生的创新精神与实践能力具有特殊作用。高等学校要重视本科的实践环节,保证实验课的开出率达到本科教学合格评估标准,并开出一批新的综合性,设计性实验。要根据科技的要求注重更新实验教学内容,提倡实验教学与科研课题相结合,创造条件使学生较早的参与科学研究和创新活动”。因此,     

二氢青蒿素对胰腺癌生长的抑制作用

Objective To investigate the anti-tumor activity of dihydroartemisinin in pancreatic cancer in vitro and in vivo. Methods For cultured cells,cell growth was determined by the MTT assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITG/PI. The protein expression in BxPC-3 cells was analyzed by Western blot assay. BxPC-3 cells were injected subcutaneously into nude mice to establish pancreatic xenograft tumors and the tumor volume was monitored after exposure to dihydroartemisinin. Ki-67 staining and TUNEL assay were used to assess tumor cell proliferation and apoptosis in tumor tissue. Results After treatment by dihydroartemisinin in vitro, the proliferative inhibition rates of pancreatic cancer cells BxPC-3 and AsPC-1 reached up to (76.2 ± 3.5) % and (79.5 ± 2.9) %, and the apoptosis rates were up to (55.5 ± 3.2)% and (40.0 ± 3.5)%, the differences were significantly (P ＜ 0.01) compared with control [(2.0 ± 1.3) % and (0.9 ± 0.4) %]. Dihydroartemisinin inhibited the growth of pancreatic xenograft tumors in nude mice. The proliferation index and apoptusis index were (49.1 ± 3.9)% and (50.2 ± 4.4)% respectively in dihydroarternisinin 50 mg/kg treatment group, compared to those of (72.1 ± 3.3) % and (9.4 ± 2.9) % in control, the differences were significantly (P ＜0.01). Western blot assay indicated that dihydroartemisinin up-regulates expression of proliferation-associated protein p21WAF1 and down-regulates expression of PCNA, increases expression of apoptosis-associated protein Bax and decreases expression of Bcl-2 and activates caspase-9 in BxPC-3 cells. Conclusions Dihydroartemisinin exerts anti-tumor activity in pancreatic cancer both in vitro and in vivo by proliferation inhibition and apoptusis induction. Dihydroartemisinin can be used as a potential anti-tumor drug in pancreatic cancer.     

胰腺类癌二例

病例1,女,33岁。以“排白色大便20 d,皮肤、巩膜黄染3 d”之主诉入院。查体:皮肤及巩膜明显黄染。腹部无发现。化验检查:谷氨酰转肽酶、碱性磷酸酶明显增高;血清总胆红素增高,以直接胆红素增高为著。B 超示肝内胆管扩张,胆总管内径1.8 cm。胰头处可探及一4.9 cm×6.5 cm低回声区,胰管全程扩张,内径1.1 cm。提示:胰头部占位性病变。上腹部 CT 示:胆总管直径达2.2 cm,其下端可见     

抑癌基因RASSFlA在胰腺癌组织中表达的研究

目的 探讨抑癌基因RASSFlA在胰腺癌组织中的表达及其与胰腺癌发生、发展的关系.方法 采用免疫印迹方法检测48例胰腺癌组织及其癌旁(正常)组织RASSFlA基因蛋白的表达水平.结果 癌旁组织RASSFlA蛋白的表达量高于高分化腺癌组织的表达量(P<0.05);高分化腺癌组织RASSFlA蛋白的表达量高于中、低分化腺癌组织(P<0.05).FNM分期Ⅲ期RASSFlA蛋白的表达量低于Ⅰ,Ⅱ期(P<0.05).RASSFlA基因蛋白表达与性别、年龄,肿瘤发生部位无明显关系(P>0.05).结论 RASSFlA表达缺失或低下在胰腺癌的发生、发展中起重要作用.     

血液滤过治疗重症急性胰腺炎的前瞻性临床研究

目的 前瞻性研究血液滤过治疗重症急性胰腺炎的临床效果.方法 遵循随机对照的实验方法,将哈尔滨医科大学第一临床医学院2004年1月至2007年8月治疗的重症急性胰腺炎37例,按发病72 h内是否接受血液滤过分为实验组(血液滤过组,n=17)与对照组(非血液滤过组,n=20),比较两组病人的APACHEⅡ评分、呼吸功能和血流动力学状态、血浆细胞因子等指标.结果 实验组病人的APACHEⅡ评分、血流动力学指标、呼吸功能、血浆细胞因子在血液滤过6 h后明显改善(P<0.05);与对照组比较有统计学意义(P<0.05);72 h后两组间差距缩小;7 d后组间监测指标比较无统计学意义;实验组和对照组病人的平均住院时间[(23.7±4.7)dvs(30.5±6.0)d]、中转手术率(17.6%vs 35.0%)和病死率(5.9%vs 15%)比较有统计学意义(P<0.05).结论 重症急性胰腺炎早期进行血液滤过可以有效改善临床症状,预防全身炎症反应综合征和多器官功能障碍综合征,缩短平均住院时间,降低中转手术率和病死率.     

甲状腺全切除术治疗良性甲状腺疾病临床价值的研究

目的:探讨甲状腺全切除术治疗甲状腺良性疾病的价值及临床意义。方法:回顾分析我院2005年3月～2007年3月收治的169例行甲状腺全切除术的甲状腺良性疾病患者临床病理资料,并对其术后并发症进行分析总结。结果:首次甲状腺全切除术后暂时性甲状旁腺功能低下和暂时性喉返神经损伤的发生率分别为0.76%和1.52%,再次手术的并发症明显增高,分别为40.54%和32.43%,P<0.01。术后患者均未发生永久性甲状旁腺功能低下和永久性喉返神经损伤。结论:甲状腺全切除术治疗良性甲状腺疾病能避免组织残留所致的病变复发和癌变,降低再手术率,且首次手术较再次手术的并发症率低,但需在术中精细操作。     

血小板活化因子乙酰水解酶对大鼠重症急性胰腺炎肺损伤的影响

目的探讨血小板活化因子乙酰水解酶(PAF-AH)对大鼠重症急性胰腺炎(SAP)肺损伤的影响。方法将45只雄性Wistar大鼠随机分为3组(n=15):sham组、SAP组和PAF-AH干预组。PAF-AH干预组于造模1、24h后经阴茎背动脉注射PAF-AH(5mg/kg体重)。各组于造模48h后测定血清中血小板活化因子(PAF)、内毒素、肿瘤坏死因子(TNF-α)含量;肺组织髓过氧化物酶(MPO)活性;对肺组织进行病理学评分。结果与SAP组比较,PAF-AH干预组血PAF(7.91±0.12)pg/L、内毒素(0.28±0.02)EU/ml、TNF-α(1.46±0.76)μg/L含量及肺组织MPO活性(5.24±1.34)U/g、肺组织病理学评分(4.88±1.35)显著降低(P<0.05)。结论应用PAF-AH可从改善微循环障碍、保护肠道屏障功能和抑制急性炎症反应等方面减轻SAP并发肺损伤。     

莫西沙星对急性重症胆管炎疗效的临床研究

目的 探讨莫西沙星对急性重症胆管炎(ACST)的有效性及安全性.方法 采用前瞻性多中心方案,选择哈尔滨医科大学和黑龙江省医院6个普通外科病房2008年l～6月ACST病人50例,在外科干预同时给予莫西沙星400mg,每日1次静脉点滴,观察入院、用药后3d和7d病人体温、WBC、谷丙转氨酶(ALT)、总胆红素(TBIL)、碱性磷酸酶(AKP)、γ-谷氨酰转肽酶(GGT)及病原菌的变化情况.结果 50例病人中1例因用药后出现肝功能异常被剔除试验,其余49例在用药后3d有42例体温和WBC明显下降,肝功能各项指标在3d时也较入院时下降,差异有统计学意义(P<0.01),临床有效率85.7%.49例中有30例培养出细菌42株,其中混合感染11例(占36.7%1,分离出大肠埃希氏菌22株(占73.3%),其次肺炎克雷伯杆菌8株,肠球菌5株,用药后7d有35株致病菌菌消失或转阴,清除率达83.3%(35/42).结论 大肠埃希氏菌、肺炎克雷伯杆菌、肠球菌是胆道感染主要致病菌.莫西沙星可有效清除胆道内病原菌,安全有效治疗ACST.     

大黄辅助早期肠内营养对急性坏死性胰腺炎病程影响的研究

Objective To investigate the effect of rhubarb assisted early enteral nutrition (EEN) on acute necrotizing pancreatitis(ANP) in rats. Methods A total of 48 Wistar rats were ran-domized into 4 groups(n=12) including ANP group (group A), rhubarb group (group B), EEN group (group C) and rhubarb assisted EEN (RAEEN) group (group D). The rats in group B, C and D were infused with rhubarb, enteral nutrient solution and enteral nutrient solution plus rhubarb by enteral nutrient canal after establishing the model, respectively. The remaining living animals in each group were sacrificed 48 hours after ANP models were developed to determine the tumor necrosis fac-tor-alpha (TNF-α), endotoxin, D-lactate, maleic dialdehyde (MDA), angiotensin Ⅱ (Ang Ⅱ) in ser-um. The intestinal secretory IgA (SIgA), the amount of ascitic fluid and the wet-dry weight ratio of ileum, the pancreatic Myeloperoxidase (MPO) activity as well as the wet-dry weight ratio of pancreas were determined and the pathologic changes of pancreas were analyzed by histopathologic grading and scoring of the severity of pancreas. Results Compared with group A, group B, C and D had signifi-cant difference in every index (P<0. 05). Furthermore, compared with rhubarb and EEN, RAEEN significantly increased the level of intestinal SIgA but reduced the other data obviously (P<0. 05). Conclusion Administration of RAEEN can effectively protect the intestinal barrier function, improve the organismic immunity, inhibit the systemic inflammatory reaction and ameliorate the microcirculato-ry disorders in ANP. The combined strategy is more safe and effective than either one alone.