NucliSens HIV-1 QT和Amplicor HIV-1 monitor1.5方法测定HIV-1病毒载量的比较研究

目的进行NuchSens HIV-1 QT和Amplicor HIV-1 monitor 1．5检测相同临床样品HIV-1病毒载量值之间的比较研究。方法收集临床样本82份，使用两种方法测定病毒载量，对病毒载量值进行统计分析。结果未测到核酸的和两种方法病毒载量对数值之差小于0．5的占88．9％；用△log10 VL〈0．5的56份样本统计两种方法的相关性，相关系数为0．956。结论NucliSens HIV-1 QT和Amphcor HIV-1 monitor 1．5两种方法测定的HIV病毒载量值有很好的相关性。     

英夫利昔单抗联合甲氨蝶呤对银屑病关节炎患者血清碱性磷酸酶水平的影响及疗效分析

目的:探讨英夫利昔单抗联合甲氨蝶呤银屑病对关节炎患者血清碱性磷酸酶水平的影响及治疗疗效。方法:选取我院2015年1月～2016年8月收治的银屑病关节炎患者66例,根据数字随机法将患者分为对照组及观察组,每组患者33例。对照组采用甲氨蝶呤进行治疗,初始剂量为7. 5 mg/周,逐渐加量至15～25 mg/周。观察组在对照组基础上联合英夫利昔单抗进行治疗,入组后在0、2、6、14、22、24周时间点输注英夫利昔单抗,每次剂量3 mg/kg。治疗24周后,比较两组患者临床疗效、治疗前后血清碱性磷酸酶水平变化情况及不良反应。结果:对照组患者治疗第14周、24周时分别有16例(48. 48%)、23例(69. 69%)患者达到Ps ARC、PASI50改善,明显低于观察组24例(72. 72%)、30例(90. 90%),差异具有统计学意义(χ2分别为4. 062、4. 694,P均0. 05);对照组治疗第24周时21例(63. 63%)达到PASI70改善,16例(48. 48%)达到PASI90改善,明显低于观察组28例(84. 84%)、29例(87. 87%)(χ2分别为3. 882、11. 803,P均0. 05);两组患者治疗后ALP水平较治疗前明显下降,其中观察组下降幅度明显优于对照组(P0. 05);两组患者在治疗过程中均未出现严重不良反应,不良反应发生差异无统计学意义(P0. 05)。结论:英夫利昔单抗联合甲氨蝶呤治疗银屑病关节炎临床疗效显著,可有效降低患者血清碱性磷酸酶水平,安全性高,值得推广。     

Plasma HIV-1 RNA decline within the first two weeks of treatment is comparable for nevirapine, efavirenz, or both drugs combined and is not predictive of long-term virologic efficacy: A 2NN substudy

BACKGROUND: The initial rate of plasma HIV-1 RNA (pVL) decline has been proposed as a marker of early efficacy of antiretroviral therapy (ART) and a possible predictor of late efficacy. We compared the rate of pVL decline in patients starting ART with nevirapine (NVP), efavirenz (EFV), or both drugs combined in addition to lamivudine (3TC) and stavudine (d4T). METHODS: Analysis of the viral decay constant (VDc) during the first 2 weeks of treatment in patients enrolled in the 2NN study who remained on allocated treatment. RESULTS: The median VDc (log10 copies per day, [interquartile range]) was similar for NVP (0.30 [0.25-0.36], EFV (0.31 [0.27-0.37]), and NVP + EFV (0.30 [0.27-0.36]). Patients with a baseline pVL >100,000 copies/mL were 8.7 (95% confidence interval [CI]: 6.2-12.3) times more likely to have a VDc >75th percentile. A high VDc was not associated with plasma drug concentration or with a decreased risk of virologic failure at week 48 after the start of therapy (hazard ratio = 0.8, 95% CI: 0.6-1.2). CONCLUSION: NVP, EFV, or NVP + EFV in combination with 3TC and d4T show similar rates of pVL decline during the first 2 weeks of treatment. The VDc with these regimens is not predictive of late virologic efficacy.     

新医学教学模式下"终身学习"教学的探索与实践

简要介绍了汕大医学院推出的“系统整合”新医学教学模式,以及在新医学教学模式中开设“终身学习”模块的原因和教学目的,重点阐述了为实现新教学模式的基本理念,在“终身学习”模块《网络基础与应用》课程中,在教学方法、教学手段等方面进行的探索与实践。     

PCR和ELISA检测单纯疱疹病毒的比较

依据ＨＳＶ ＤＮＡ多聚酶基因核苷酸序列设计并合成一对引物，建立了ＰＣＲ方法。用ＰＣＲ和ＥＬＩＳＡ平行检测ＨＳＶ－１，ＨＳＶ－２标准毒株感染的细胞，拟诊ＨＳＶ脑炎患者脑脊液（ＣＳＦ）及多种对照标本。结果表明，两方法特异性完全一致；ＰＣＲ检测阳性率高，比ＥＬＩＳＡ敏感近２０倍，更适用于ＨＳＶ脑炎的早期诊断。     

Human neutrophils secrete gelatinase B in vitro and in vivo in response to endotoxin and proinflammatory mediators

Bacterial sepsis is characterized by a systemic inflammatory state, with activation of numerous cell types. Phagocytes participate in this phenomenon by secreting various proinflammatory cytokines and enzymes. Matrix metalloproteinases (MMPs) such as gelatinases are produced by phagocytes and are thought to play an important role in processes of cell transmigration and tissue remodeling. In this work, we show that endotoxin (lipopolysaccharide [LPS]) and other inflammatory mediators, such as tumor necrosis factor (TNF), interleukin-8, and granulocyte colony-stimulating factor, induce a rapid (within 20 min) release of gelatinase-B (MMP-9) zymogen in whole human blood, as determined by gelatin zymography. The polymorphonuclear neutrophil was identified as the cell responsible for this rapid secretion, as a result of the release of preformed enzymes stored in granules. Normal human subjects given LPS intravenously showed a similar pattern of proMMP-9 secretion, with maximum plasma levels reached 1.5 to 3 h after LPS administration (P = 0.0009). Prior administration of TNF receptor:Fc, a potent TNF antagonist, to subjects given LPS, only partially blunted the release of proMMP-9 (P = 0.033). Ibuprofen, a cyclooxygenase inhibitor, did not alter this pattern of release. Increased levels of proMMP-9 and proMMP-2, as well as activated forms of MMP-9, were found in plasma from two patients with gram-negative sepsis. The levels of MMPs paralleled the severity of clinical condition and a marker of the severity of sepsis, plasma procalcitonin. These data indicate that MMPs are released in whole blood in response to various inflammatory mediators and that they could serve as sensitive and early markers for cell activation during the course of bacterial sepsis.     

茶色素治疗糖尿病前后血液流变性和微循环检测分析

目的：观察茶色素治疗糖尿病前后血液流变微循环的变化。方法：糖尿病人88例，男71例，女17例，年龄32～64岁。口服茶色素2粒（250mg），每日3次，疗程为30天。结果：血液流变学参数、微循环积分值、血生化指标、RIA都有明显改善（P＜0．01或P＜0．05）。结论：茶色素可以明显改善糖尿病人血液流变性和微循环多项检测指标，值得临床推广应用。     

采用对象模型技术构建医院信息系统目标

医院信息系统建设的首要问题是建设目标的确立问题，如何确立医院信息系统的建设目标并有计划地实现目标是医院信息系统建设成功的关键。本文通过对多家医院信息系统的考察并结合自身医院建设经验，提出了采用对象模型技术确立医院信息系统建设目标的新方法。     

The Effects of Extracellular Matrix on Tissue Engineering Construction of Cartilage in Vitro

In the study of bone and cartilage diseases in our laboratory, chondrocyte stereo-differentiation mod-el was cultured into cartilage tissue. The cultured cartilage tissue was closely similar to natural tissue inboth histological condition and biochemical metabolism. The model mimicked the chondrocyte differenti-ation and metabolism in vivo completely〔1〕. We studied some essential extracellular matrices, includingcollagen, proteoglycan(PG) and hyaluronic acid(HA) on the construction of grow…