18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.     

腹腔镜下缝扎切开取胚法治疗输卵管间质部妊娠

目的探讨腹腔镜下缝扎切开取胚法治疗输卵管间质部妊娠的效果。方法2004年7月～2007年12月32例输卵管间质部妊娠按照不同手术方法分为2组。A组16例,腹腔镜下采用缝扎切开取胚法施行输卵管间质部切除术;B组16例,腹腔镜下采用局部电凝后施行输卵管切除术,比较2组手术时间、术中出血量、术后并发症、术后24h血β-hCG值变化、血β-hCG值恢复正常时间。结果A组手术时间（40±16）min显著少于B组（60±19）min（t=-3.178,P=0.004）;A组术中出血量（40±12）ml显著少于B组（95±26）ml（t=-7.644,P=0.000）,2组术后24h血β-hCG值变化差异无显著性[（9889±7945）mIU/mlvs（9696±7883）mIU/ml,t=0.068,P=0.946],A组血β-hCG值恢复正常时间（10.1±3.2）d与B组（9.8±2.4）d无统计学差异（t=0.294,P=0.771）。2组均无持续性异位妊娠发生。结论腹腔镜下缝扎切开取胚法治疗输卵管间质部妊娠疗效肯定。     

联合检测血清VEGF、P和PAPP-A在早期诊断异位妊娠中的应用

目的?探讨联合检测血清中血管内皮生长因子（VEGF）、孕酮(PRG）、妊娠相关蛋白A（PAPP-A）在异位妊娠早期诊断中的应用。方法?选取2011年4月至2012年9月我院妇产科门诊和住院病例138例，分为正常宫内妊娠组（44例）、异常宫内妊娠组（45例）和异位妊娠组（49例），放免法测定血清中P水平，ELLASA法测定VEGF和PAPP-A水平。结果?P水平在正常妊娠、异常宫内妊娠及异位妊娠依次下降，3组之间差异均有显著性(P<0.05)；VEGF水平在正常妊娠与其余两组之间差异有显著性(P<0.05)；PAPP-A水平在异位妊娠与另外两组之间差异有显著性(P<0.05)。如将三者联合成式子[VEGF/(PAPP-A×P)]检测3组之间均差异有非常显著性(P<0.01)。结论?血清孕酮与PAPP-A、VEGF对异位妊娠的早期诊断有一定价值，三者联合测定将进一步提高早期异常妊娠的诊断率。     

隔衣测量血压对测量值的影响研究

目的：探讨血压计袖带缠绕上肢时隔衣物测量血压对血压测量值的影响。方法：以裸臂法为对照组,隔薄衣法和隔厚衣法为试验组。分别测量同一组研究对象的同侧上肢肱动脉血压,3种方法测得的血压值采用方差分析的方法进行统计学分析。结果：对照组与试验组1（隔薄衣法）测得的收缩压和舒张压值无统计学意义（P〉0.05）。对照组与试验组2（隔厚衣法）测得的收缩压和舒张压值在统计学上差异均有显著性。试验组2测得的收缩压和舒张压值均高于对照组。收缩压的平均差值为4.440mmHg（1 mmHg=0.133 kPa）,差值的95%置信区间为1.408～7.472 mmHg。舒张压的平均差值为4.640 mmHg,差值的95%置信区间为1.365～7.915 mmHg。结论：医护人员在为无血压异常病史的患者测量血压时,可以隔一层厚度〈1 mm的衣袖缠绕袖带进行测量,该方法对血压测量的结果不会有影响。     

人胎盘绒毛膜间充质干细胞分离培养的新方法

建立一种简便、快捷的人胎盘绒毛膜间充质干细胞(human placental chorionic-derived mesenchymal stem cells,hpc MSCs)分离培养方法。用手持电动匀浆器处理人绒毛膜,经酶消化后接种培养,用倒置显微镜观察细胞形态,CCK-8测定细胞生长曲线,流式细胞术检测细胞表面标记,成脂、成骨诱导分化试剂盒鉴定细胞的分化潜能。获得的人胎盘绒毛膜间充质干细胞为成纤维细胞样细胞,呈平行排列或漩涡状生长,高表达CD73、CD105、CD90,而CD34、CD45、CD14、CD19、HLA-DR呈阴性,生长曲线为"S"型,经成骨或成脂诱导后,茜素红染色或油红O染色呈阳性。符合国际细胞治疗学会认可的间充质干细胞标准。建立了人胎盘绒毛膜间充质干细胞的分离培养新方法,结合了组织块法和酶消化法的优点,为绒毛膜间充质干细胞的应用提供基础。     

38例早发型子痫前期重度临床分析

目的探讨早发型子痫前期重度的治疗及母儿结局。方法对38例不同孕周的早发型子痫前期重度进行回顾性分析。根据发病孕周分成A组(孕周<28周)、B组(28周≤孕周<32周)、C组(32周≤孕周<34周)。结果B组期待治疗的时间显著长于其他两组(P<0.05);B组、C组分娩方式以剖宫产为主;三组间患者并发症发生率无显著差异(P>0.05);三组间新生儿窒息发生率及围生儿死亡率有显著差异(P<0.05),随着孕周延长而下降。结论早发型子痫前期重度新生儿窒息发生率和围生儿死亡率随发病孕周延长而降低,期待治疗过程中应严密检测母婴情况,适时选择合适的终止妊娠的方式。     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.     

整改通知书反馈法在护理质量缺陷管理中的应用

[目的]探讨有效的护理质量控制信息及反馈方法.[方法]通过查阅文献、访谈制定整改通知书并实施.比较实施前后27个临床科室和6个特殊科室的护理质量控制项目、护理缺陷重复发生率、整改措施内涵质量.[结果]实施后各项比较指标明显好转.[结论]整改通知书反馈法值得临床推广应用.     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.