CO2气腹环境对胃癌细胞黏附与侵袭转移的影响

Objective To investigate the influence of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expression of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cells MKN-45, SGC-7901 and MKN-28 were exposed to 3 different CO2 gradients: 9 mm Hg, 15 mm Hg and control group (0 mm Hg). The expression of E-cadherin, ICAM-1, MMP-2 and VEGF-A were measured at 2 and 4 hours exposure by using RT-PCR, CytoMatrixTM kit and ECMatrixTM kit. The pretreated gastric cancer cells were injected into abdominal cavity of nude mice(2×106 cells per mouse). Five mice in each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept for observation of survival time. Results The expression of E-cadherin (MKN-45: from 2.26 to 2.19, SGC-7901 :from 2.16 to 2.09、MKN-28 :from 2.06 to 1.99), ICAM-1 (MKN-45 : from 2.20 to 2.28、SGC-7901: from 2.10 to 2.18、MKN-28: from 2.00 to 2.08), MMP-2 (MKN-45:from 2.05 to 2.13、SGC-7901: from 1.95 to 2.03、MKN-28: from 1.85 to 1.93) and VEGF-A(MKN-45 : from 2.10 to 2.16、SGC-7901 :from 2.00 to 2.06、MKN-28: from 1.90 to 1.96) didn't change significantly with increasing pressure and time (P>0.05). The expression of adhesive and invasive molecules didn't change significantly between the experimental groups and the control group. There was no statistical significance of tumor metastasis in abdominal cavity of nude mice(MKN-45:from 22 to 23、SGC-7901 :from 20 to 22、MKN-28:from 21 to 22) and survival time(MKN-45 :from 23 to 21、SGC-7901 :from 22 to 21、MKN-28 :from 22 to 21) among all the groups. Conclusion Under low pressure and short time of CO2 exposure, the adhesive and invasive capacity of gastric cancer cells did not change significantly hence did not increase the possibility of neoplasm metastasis.     

腋窝淋巴结异位皮肤附属器伴毛源性肿瘤1例报道

目的 总结腋下淋巴结异位皮肤附属器伴毛源性肿瘤患者的诊断和治疗经验。方法 回顾性收集上海交通大学医学院附属仁济医院南院区普外科收治的1例腋下淋巴结异位皮肤附属器伴毛源性肿瘤患者的临床和病理资料。结果 患者为一63岁女性，因“扪及左侧腋下肿块且逐渐增大1+个月”就诊。术前穿刺组织病理学活检结果提示：皮肤附属器伴毛源性分化肿瘤可能。采用手术完整切除肿块，术后石蜡组织病理检查结果：淋巴组织内增生的鳞状上皮伴多发小囊肿形成；免疫组织化学染色结果：上皮样细胞角蛋白（+），细胞角蛋白34βE12（+），雌激素受体（–），孕激素受体（–），人表皮生长因子受体2（–），Ki-67(10%+)。术后病理诊断：淋巴结内异位皮肤附属器组织伴毛源性肿瘤形成。术后恢复顺利，近期无复发。结论 腋窝淋巴结异位腺体少见，异位皮肤附属器伴毛源性肿瘤更罕见，此需与乳腺癌腋窝淋巴结转移、副乳腺癌、纤维腺瘤等鉴别，完整手术切除为其主要治疗手段。     

8例直肠和乙结肠急性炎症性穿孔临床分析

直肠、乙结肠急性炎症性穿孔临床上较为少见,术前明确诊断较为困难.但是病情进展迅速,若诊治不及时或治疗不当,严重的腹腔内感染有较高的病死率[1].本文回顾分析1998年7月至今本院收治的8例直肠、乙结肠急性炎症性穿孔患者的临床资料,以分析其临床特点及治疗方法.     

胃癌发生和发展的分子生物学基础

胃癌是常见的恶性肿瘤之一，它的发生与发展是一个极其复杂的过程，近年来对其研究比较深入，涉及基因、细胞周期、多态性、端粒酶、细胞因子和细胞黏附分子等一系列的变化。现就其分子水平上的一些变化作一综述。     

CO2气腹环境对胃癌细胞黏附与侵袭转移的影响

Objective To investigate the influence of CO2 pneumoperitoneum on the adhesive and invasive ability of gastric cancer cells based on the expression of adhesive and invasive molecules. Methods With an artificial CO2 pneumoperitoneum model in vitro, human gastric cancer cells MKN-45, SGC-7901 and MKN-28 were exposed to 3 different CO2 gradients: 9 mm Hg, 15 mm Hg and control group (0 mm Hg). The expression of E-cadherin, ICAM-1, MMP-2 and VEGF-A were measured at 2 and 4 hours exposure by using RT-PCR, CytoMatrixTM kit and ECMatrixTM kit. The pretreated gastric cancer cells were injected into abdominal cavity of nude mice(2×106 cells per mouse). Five mice in each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity. The remaining mice were kept for observation of survival time. Results The expression of E-cadherin (MKN-45: from 2.26 to 2.19, SGC-7901 :from 2.16 to 2.09、MKN-28 :from 2.06 to 1.99), ICAM-1 (MKN-45 : from 2.20 to 2.28、SGC-7901: from 2.10 to 2.18、MKN-28: from 2.00 to 2.08), MMP-2 (MKN-45:from 2.05 to 2.13、SGC-7901: from 1.95 to 2.03、MKN-28: from 1.85 to 1.93) and VEGF-A(MKN-45 : from 2.10 to 2.16、SGC-7901 :from 2.00 to 2.06、MKN-28: from 1.90 to 1.96) didn't change significantly with increasing pressure and time (P>0.05). The expression of adhesive and invasive molecules didn't change significantly between the experimental groups and the control group. There was no statistical significance of tumor metastasis in abdominal cavity of nude mice(MKN-45:from 22 to 23、SGC-7901 :from 20 to 22、MKN-28:from 21 to 22) and survival time(MKN-45 :from 23 to 21、SGC-7901 :from 22 to 21、MKN-28 :from 22 to 21) among all the groups. Conclusion Under low pressure and short time of CO2 exposure, the adhesive and invasive capacity of gastric cancer cells did not change significantly hence did not increase the possibility of neoplasm metastasis.     

门静脉高压症诊治中磁共振血管成像与间接门静脉造影和多普勒超声对照研究

目的:将磁共振血管造影术(MRA)与间接门静脉造影术(IPVG)和多普勒超声检查(DUS)对门静脉系统解剖影像学及血流动力学的检查结果进行比较,探讨MRA在门静脉高压症诊治中的应用价值。方法:于38例肝硬化门静脉高压症病人和12例非肝硬化病人对照组进行3D-DCEMRA和2D-PCMR检查,检测门静脉系统解剖的显像和血流量,分别与IPVG和DUS的结果进行比较。结果:MRA能很好显示门静脉系统的各属支,成像结果优于IPVG。对门静脉系统各血管流速、流量的测定与DUS有良好的相关性。结论:MRA能较好显示门静脉系统的解剖影像,并可测得血流动力学资料,可成为门静脉高压症血流动力学研究的首选方法。     

磁共振血管造影在门静脉高压症外科中的临床应用

目的 将磁共振血管造影（MRA）与多普勒超声对门静脉系统解剖影像及血流动力学的检查结果作对比,评价MRA在门静脉高压症外科中的应用价值。方法 对肝硬变门静脉高压症组病人包括行手术者和非肝硬变对照组进行3D－DCE MRA（三维动态对比增强磁共振血管造影）和2D－PC MR（二维相位对比磁共振）检查,检测门静脉系统解剖显像和血流量,后者与多普勒超声（DUS）进行比较。结果 3D－DEC MRA能较好显示PV、SV、SMV和头向侧支以及分流术的吻合口。2D－PC MR对门脉系统血流量的测定与DUS无显著差别。结论 MRA为创性检查,能较好地显示门静脉系统的解剖影像,可与传统X线血管造影媲美,并可测得血流动力学资料,在门静脉高压症外科中具有应用价值。     

铁皮石斛试管繁殖降低成本的研究

研究结果表明,铁皮石斛壮苗阶段,用自来水代替蒸馏水,食用白砂糖代替分析纯蔗糖,去除培养基中的微量元素和有机物来配制培养基可降低培养基成本10%～15%。     

肝移植免疫耐受的研究进展

通常情况下，肝移植后的排斥反应发生率及发生程度远比其它实质性脏器低，并且移植肝可诱导受者对供者其他易排斥的器官产生免疫耐受，如心、肾、皮肤和胰腺等。这种现象说明移植肝能以某种反应形式与宿主的免疫系统发生相互作用，创造出免疫耐受的环境，即肝脏具有所谓内在的“耐受原性”。