聚合酶链反应快速诊断新生儿淋菌性眼炎临床体会

新生儿淋菌性眼炎系由淋病奈瑟氏球菌(NG)引起的眼科急症，必须及时治疗。我院对1997~2000年收治的47例新生儿眼炎的结膜分泌物同时作聚合酶链反应(Polymerase Chain Reaction，PCR)检测、涂片染色和细菌培养法检测，现示出PCR具有敏感、特异、快速的独特优点。 1 一般资料 本     

灌服浓盐水配合注射硫酸钠液治疗牛瓣胃阻塞效果好

灌服浓盐水配合注射硫酸钠液治疗牛瓣胃阻塞效果好高超（山东畜牧兽医学校）牛瓣胃阻塞（又称百叶干）是较为严重的前胃疾病之一。多因冬春两季长期吃单一的质量较差的粗纤维饲料，加以初春天气干燥及饮水不足．致使前胃运动机能减弱，食物在瓣胃内停滞、变干而引起。瓣胃...     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

利培酮对精神分裂症患者血清细胞因子的影响

20世纪80年代以来，国外有关精神分裂症与细胞因子的相关性研究表明，精神分裂症患者存在多种细胞因子功能异常，但对于抗精神病药物对细胞因子的影响研究较少。本研究旨在探讨利培酮对精神分裂症患者血清白介素6(IL-6)、白介素8(IL-8)、r干扰素(IFN-γ)和肿瘤坏死因子α(TNF—α)的影响，并探讨精神症状、药物副作用与细胞因子的关系。     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

支气管哮喘炎性反应的组织学证据——支气管粘膜的超微结构观察

为确定支气管哮喘患者支气管粘膜的组织学特征和炎性反应的程度,我们对5例哮喘病人和3例非哮喘对照病人经支纤镜取支气管粘膜进行超微结构检查。发现哮喘病人支气管粘膜固有层嗜酸性细胞浸润,支气管上皮细胞脱落,上皮基底膜增厚和裸露,纤毛上皮细胞脱落,上皮基底膜下胶原沿着和杯状细胞增生。我们的结果提示支气管哮喘气道内有广泛的炎性改变,支持支气管哮喘是一种炎性疾病的观点。     

表达端粒酶逆转录酶siRNA的溶瘤腺病毒对裸鼠肾癌移植瘤的治疗作用

Objective To investigate the antitumor effect of oncolytic adenovirus armed with small interference RNA targeting hTERT gene for renal cancer therapy. Methods Nude mice were divid-ed randomly into 4 groups (8 mice/group),and were treated by intratumoral injections of ZD55-hTERT ( an oncolytic adenovirus armed with small interference RNA targeting hTERT gene) ,ZD55-EGFP ( an on-colytic adenovirus) and Ad-hTERT (replication-defective adenovirus armed with small interference RNA targeting hTERT gene) with three consecutive daily at 7 × 108 pfu/day or treated with PBS as a control. The expression of E1A and hTERT, and apoptosis of tumor xenografts were assessed by immunohistochemi-cal technique at the 7th day after injections. The tumor volume was measured at the 50th day after injec-tions. Results The tumor volume in ZD55-hTERT treatment group ( 124.1±27.5) was significantly less than that in ZD-EGFP (499.8±77.1 ) and Ad-hTERT ( 609.0±102.5 ) treatment groups. The E 1A pos-itive expression in ZD55-hTERT treatment group was significantly higher than that in Ad-hTERT treatment group. The hTERT positive expression in ZD55-hTERT treatment group was significantly lower than that in Ad-hTERT treatment group. ZD55-hTERT treatment of tumor xenografts resulted in an increased apoptotie cell death as compared with ZD55-EGFP and Ad-hTERT treatment. Conclusion The antitumor effect of ZD55-hTERT was more potent than oneolytie adenovirus ZD55-EGFP and Ad-hTERT.     

介于良－恶性之间的少枝胶质细胞瘤1例报告

 患者男，35岁。患者自觉左侧耳鸣2个月，瘫痛发作一次，余无其它不适。检查：临床上无阳性体征发现。     

内皮抑素及其基因治疗肿瘤的新进展

1971年Ｆｏｌｋｍａｎ教授首次提出“肿瘤的生长依赖于血管生成 ,新血管的形成是体积 >1～ 2ｍｍ3 实体瘤生长和癌细胞转移的前提条件”。从此抑制肿瘤血管的生成成为肿瘤治疗学研究的新热点。 1997年Ｏ’Ｒｅｉｌｌｙ[1] 首次从鼠的血管内皮细胞瘤 (Ｅｏｍａ)培养液中提取出内皮抑素———一种新的血管生成抑制因子。经过 5年的实验动物研究和Ⅰ期临床试验证明 :内皮抑素能有效抑制血管内皮的增殖 ,促使肿瘤细胞的凋亡 ,抑制肿瘤的生长和转移 ,具有很强的抗肿瘤特性 ,而且无耐药性和毒副作用[1] 。本文就内皮抑素及其基因治疗肿瘤的研究应…