艾灸对实验性RA大鼠血浆Th1/Th2细胞因子平衡影响的实验研究

目的:观察艾灸对实验性类风湿关节炎(Rheumatoid arthrids,RA)大鼠血浆IL-1β、IL-4含量的影响,探讨灸法对类风湿关节炎的细胞因子平衡的影响.方法:以佐剂性关节炎(从)作为RA疾病模型.艾灸实验性RA大鼠"肾俞"、"足三里"两穴,运用ELISA双抗体夹心法测定治疗前后大鼠血浆中IL-1β、IL-4含量.结果:大鼠血浆中IL-1β的含量,模型组显著高于空白组(P<0.01),艾灸组显著高于空白组但也显著低于模型组(P<0.01).大鼠血浆中IL-4含量,空白组低于模型组,但两组之间无显著性差异,艾灸组高于模型组与空白组,与这两组之问存在显著性差异(P<0.05).大鼠血浆IL-1β/IL-4比值,模型组显著高于空白组(P<0.05),艾灸组显著低于模型组(P<0.05),与空白组无显著性差异.结论:艾灸能够抑制RA血浆中的IL-1β分泌并同时促进RA血浆中IL-4的分泌,调整Th1/Tn2细胞因子失衡,对恢复RA病程中出现的Th1/Tn2细胞因子失衡,治疗RA慢性炎症以及RA预后有积极意义.     

神阙穴古今临床应用探析

<正>神阙穴,又称"脐中"、"气舍"、"下丹田"、"命蒂"等,始见于《素问·气穴论篇》"藏俞五十穴,……齐一穴,……针之所由行也",但最早提出"神阙"二字的是晋·皇甫谧《针灸甲乙经》。《会元针灸学》载:"神阙者,神之所舍其中也。……脐居正中,如门之阙,神通先天。父母相交而成胎时先生脐带,形如荷茎,系于母之命门,天一生水而生肾,状如未放莲花,顺五行以生土,赖母气以相转,十月胎满,则神注脐中而成人,故名神阙。"本文基于神     

针灸治疗复发性口腔溃疡的选穴特点及分布规律分析

通过检索针灸治疗复发性口腔溃疡(recurrent oral ulcers,ROU)的相关文献,总结其选穴的特点和穴位分布规律,拟为针灸治疗ROU的基础和临床研究科学合理选穴提供理论依据。结果显示:针灸治疗复发性口腔溃疡选穴频次最高为足三里穴,其次为合谷、三阴交、颊车等穴。针灸治疗ROU选穴突出了近治和远治作用、整体和局部相结合的特点,且以足阳明胃经、手阳明大肠经、足太阴脾经及足少阴肾经为主的分布规律,体现了中医针灸"调整阴阳,扶正祛邪"思想在治疗复发性口腔溃疡治疗中的重要性。     

艾灸对类风湿性关节炎家兔关节滑膜液IL-1β、TNF-α含量的影响

目的观察艾灸对实验性类风湿性关节炎(Rheumatoid arthritis,RA)家兔关节滑膜液IL-1β、TNF-α含量的影响,探讨灸法治疗类风湿性关节炎的作用机理.方法以佐剂性关节炎(AA)作为RA疾病模型,艾灸实验性RA家兔双侧"肾俞"穴,运用ELISA双抗夹心法测定治疗前后家兔关节滑膜液IL-1β、TNF-α的含量.结果 RA模型组关节滑膜液中IL-1β、TNF-α含量显著高于空白对照组 (P＜0.05);艾灸治疗组关节滑膜液中IL-1β、TNF-α含量明显低于RA模型组(P＜0.05, P＜0.01).结论艾灸治疗能抑制RA滑膜细胞分泌IL-1β、TNF-α,对抑制滑膜炎症有重要作用.     

免疫刺激对大鼠海马CA2-3区GR表达的影响

目的探讨细菌脂多糖(LPS)对大鼠海马CA2-3区糖皮质激素受体(GR)表达的影响。方法将健康雄性SD大鼠10只随机分为实验组和对照组,每组5只,实验组大鼠腹腔注射LPS,对照组大鼠腹腔注射等量生理盐水,用免疫组化方法测定大鼠海马CA2-3区GR的表达。结果 GR在大鼠海马各区散在分布,实验组大鼠海马CA2-3区GR表达量明显低于对照组(P<0.01)。结论海马CA2-3区GR参与调节LPS诱导的免疫反应过程。     

脑深部电刺激双侧伏核对吗啡成瘾大鼠复吸行为的影响

Objective To investigate the influence on the behavior of withdrawal and relapse after deep brain stimulation of bilateral nucleus accumbens in morphine-dependent rats. Methods The rats with a strong unconditioned preference were discarded in preconditioning test, the selected rats were distributed into five groups randomly. After operation,morphine hydrochloride was injected subcutaneously into SD rats for 12 days (once every day,initial 5 mg/kg,increasing by 5 mg/kg per time,stable in 20 mg/kg ). A modified electrical circuit was used to procedure the DBS,the parameter was 130 Hz,150 A,60 s,l h/d,14 d. CPP test was used to exam the effect of DBS. A minor morphine dose (3 mg/kg) was injected to induce the behavior of relapse, and CPP was tested again after 24 h. Two-way ANOVA was performed on the data with Bonferroni posttest. Result ①After CPP training,CPP score of group morphine, morphine + sham and morphine + DBS was ( 155. 87 ± 20. 45 ) s, (107.33 ± 18.10)s,(135.45 ±22.09)s,and had significant difference with group of control( ( -70.34 ± 15.40) s)(t = 9.45,P＜0.01; t = 6.94,P＜0.01;t = 8.04,P＜0.01).②After 7 days' DBS,the CPP score in group of morphine + DBS reduced significantly compared to group of morphine( t = 4.21, P＜0.01) and morphine + sham( t=1.10, P＜0.05).0n the 14th day,there was more pronounced reduction ( t = 5. 15, P＜0.01; t = 3.92, P＜ 0.01). ③ 24 hours after the minor morphine dose was injected,the CPP score in morphine + DBS didn't increase significantly, and had significant difference with group of morphine ( t = 4.04, P＜0.01) and morphine + sham ( t= 4. 13, P＜0.01). Conclusion DBS bilateral nucleus accumbens in morphine-dependent rats can interfere the behavior of morphine-induced CPP and relapse.     

股骨颈骨折两种治疗方法的比较

目的　探讨闭合复位内固定与切开复位内固定治疗股骨颈骨折的疗效、各自优缺点及最佳适应症。方法　将 98例股骨颈骨折患者随机分为两组 ,分别采用闭合复位内固定术及切开复位内固定术治疗。其中闭合复位组 56例 ,切开复位组 42例。结果　经 1～ 5年 ,平均 2 6年的随访。闭合复位组 :治愈 33例 ,好转 2 0例 ,未愈 3例 ;切开复位组 :治愈 2 1例 ,好转 1 9例 ,未愈 2例。两种治疗方法临床效果无统计学差别 (P >0 0 5)。结论　对能闭合复位满意的新鲜股骨颈骨折 ,闭合复位内固定应视为有效、可靠的首选方法 ;对陈旧性股骨颈骨折及股骨颈骨折颈后骨质粉碎缺损、经复位后对位又不满意者 ,则应首选切开复位内固定结合带蒂骨瓣移植治疗     

肥胖患者异丙酚静脉全麻行无痛肠镜中麻醉并发症临床观察

众所周知,过度肥胖者心脏负荷增大,心、肺疾病易患率增加。因而过度肥胖者在麻醉中对心脏、呼吸系统有影响的药物应用时应监测循环、呼吸系统的各项指标。本研究通过对肥胖患者异丙酚静脉全麻术行结肠镜检查中麻醉并发症的发生情况进行观察,探讨肥胖在异丙酚静脉全麻中对心脏、呼吸系统的影响。