大白鼠骨髓粒系祖细胞(GM-CFU-C)的体外琼脂培养

目前对多种动物的粒系祖细胞(GM-CFU-C)测试技术研究和应用日趋广泛。但是,迄今对大鼠骨髓GM-CFU-C体外琼脂培养的研究方法国内未见公开报道,国外有关文献也为数不多。我们在小白鼠骨髓GM-CFU-C体外琼脂培养方法的基础上,进行了较系统的探索,初步建立起     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

Herbst矫治器与方丝弓矫治器双期矫治AngleⅡ~2类错的硬组织改变

目的探讨铸造夹板式Herbst矫治器和方丝弓矫治器双期矫治AngleⅡ2类错的机制和效果。方法选取30例FG-G期恒牙列AngleⅡ2类错患者,平均分为Herbst治疗组和对照组,两组患者均采用非拔牙矫治。其中,Herbst组15例,采用铸造夹板式Herbst矫治器和方丝弓矫治器双期矫治;对照组15例,采用方丝弓矫治器辅以头帽口外弓后推磨牙治疗。Herbst组和对照组的所有患者均在治疗前、后拍摄头颅定位侧位片,进行矫治前后、颌、面矢状向和垂直向上的头影测量分析,并做统计学处理。结果两组患者均达到了磨牙中性关系,前牙达到正常的覆盖、覆关系,但是治疗组的疗程明显的短于对照组。结论方丝弓矫治器固定矫治法和Herbst矫治器、方丝弓矫治器双期矫治法均能有效地矫治AngleⅡ2类错;但前者主要通过牙齿的变化发挥矫治效能,而后者则主要通过Herbst矫治器刺激下颌骨生长、促进下颌骨前移等骨性变化发挥矫治效能、并且有利于患者面型的改善。     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

安氏Ⅱ^1类错He上颌尖牙远中移位后切牙及磨牙位置变化的研究

目的探讨安氏Ⅱ1类错拔牙矫治中,上下颌磨牙及切牙的位置变化。方法选择15名11－16岁拔除四个第一双尖牙的安氏Ⅱ1类错病例,用方丝弓矫治技术,口外力加强上颌磨牙支抗,治疗前及以间断力后移上颌尖牙到与第二双尖牙接触时,进行X线头影测量及统计分析。结果上颌磨牙前移了0.9mm,比下颌磨牙整体前移少约2mm;上切牙舌侧斜移动了4.1mm;下切牙舌向移动了1.8mm,比上颌磨牙前移少1mm。结论控制上颌磨牙支抗并增大弓丝的滑动,可使上颌切牙在未主动内收前舌向移动;减少下颌尖牙的后移则有利于下颌磨牙的前移。     

凝血功能对急性肾功能衰竭早期检测的意义

目的：探讨凝血功能在早期急性肾功能衰竭中的变化,说明凝血功能在急性肾功能衰竭中的意义。方法选择2011年10月-2014年3月粤北人民医院肾内科和儿科收治的急性肾功能衰竭患者80例为观察组,同期体检中心健康体检者50例为对照组,比较两组肌酐、尿素氮、血小板、活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、凝血酶时间（TT）、血浆纤维蛋白原（FIB）的水平。结果观察组FIB、血小板水平明显高于对照组（P＜0.05）,而APTT、PT、TT差异无统计学意义（P＞0.05）。结论早期急性肾功能衰竭患者血液呈高凝状态,凝血功能异常可能参与急性肾功能衰竭的形成。     

椎管内外神经节细胞瘤一例

椎管内外神经节细胞瘤一例陈立华，陈善成，张明宇，伍军，周艳红患者女，３６岁。因颈部疼痛８年，四肢无力６年，进行性四肢活动障碍１年，大小便失禁１个月，双下肢完全瘫痪１４天于１９９４年１１月１日入院。入院诊断：神经纤维瘤Ｃ１～３。体查：Ｃ１～３脊椎有叩击...     

西瓜霜润喉片治疗咽喉、口腔疾病326例疗效观察

西瓜霜润喉片是佳林中药制药厂独家生产的名优产品,在国内外亨有盛誉,该药适用于咽喉、口腔疾病的治疗和预防。为进一步验证其疗效,我们于1900年10月至1992年9月对326例服用西瓜霜润喉片者进行临床观察,现将结果报告如下。 1 临床资料 326例中,男137例,女189例;年龄2～80岁。平均36岁。门诊病人285例,住院病人41例,所患疾病见附表。     

重型颅脑损伤合并寰枢椎半脱位的诊断与治疗

目的探讨重型颅脑损伤合并寰枢椎半脱位的诊断和治疗方法。方法重型颅脑损伤137例患者中合并寰枢椎半脱位者17例。每例患者均行颅脑和寰枢椎CT扫描，必要时加做寰枢椎CT三维重建或MRI检查。在积极治疗颅脑损伤同时，均予以颈同固定制动、颌枕吊带或颅环弓牵引治疗。结果17例重型颅脑损伤合并寰枢椎半脱位患者经治疗后12例神志转清，5例植物生存。2例有脊髓损伤者完全恢复。无1例因寰枢椎半脱位而导致呼吸骤停或猝死，CT复查寰枢椎结构恢复正常。结论对重型颅脑损伤患者在颅脑伤诊治的同时，应常规行寰枢椎CT检查，以明确诊断，早期治疗，能有效预防并发症，改善预后。     

恒牙期安氏Ⅱ^1类下颌后缩的Herbst矫治器矫治及Edgewise的正畸治疗

患者为男性,14 .3岁,身高1.75m。主诉:上前牙不齐、前突求治。无不良习惯史,全身状况良好,生长发育正常;其母亲下颌后缩,下颌平面斜度较大。临床检查:1 患者正侧位外貌特征:面下1/ 3略小,颏部轻度右偏,唇闭合时左上中切牙稍外露;下颌后缩,颏唇沟深。2 牙列情况:恒牙;右侧磨牙完全远中关系,左侧磨牙远中尖对尖关系,双侧尖牙完全远中关系;前牙深覆Ⅲ°、深覆盖11.5mm ;上前牙区拥挤Ⅰ°,上中线居中,下中线右偏2 .5mm。个别牙齿情况:11近中稍腭向、2 1唇向、2 4远中稍舌向转位、34稍颊向、4 3舌向低位欠隙。口腔卫生情况良好,牙龈组织健康…