Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

浅析托槽黏结强度的影响因素

在正畸固定矫治中,托槽黏结是矫治过程中的常规操作。临床上黏结托槽的步骤一般为：清洁牙面、酸蚀牙面、涂布底涂剂、涂布糊剂、最后黏结托槽于牙面。牙面通过黏结剂与托槽形成整体,弓丝则通过托槽对牙施以各种矫治力。然而,在矫治中时常出现托槽脱落的现象,导致矫治力中断,对矫治疗程及效果产生很大影响。因此,托槽黏结强度越来越受到正畸医生的重视。影响托槽黏结强度的相关因素有：牙面处理、黏结剂的类型和托槽等。本文总结近年来学者们对托槽黏结强度的研究,针对托槽在黏结过程中所涉及的影响因素进行综述。     

干细胞治疗影像监测方法的研究进展

干细胞治疗在糖尿病、心血管病、恶性肿瘤、组织损伤等疾病中展现了潜在的应用价值。高效非创伤性监测干细胞移植方法将加速干细胞治疗技术的发展。概述了核磁共振成像和放射性核素显像监测干细胞治疗的研究进展。     

放线菌性硬膜外脓肿1例并文献复习

正放线菌病是一类由放线菌引起的慢性化脓性肉芽肿性侵袭性感染。该病临床较少见,可发生于身体任何部位,放线菌性颅内感染临床上更为少见,文献仅有个例报道,易被误诊为细菌性颅内感染或肿瘤。本文报道我院收治的1例重型脑外伤术后并发放线菌性硬膜外脓肿病例,同时复习相关文献,以提高临床医师对该病的临床特点认识及诊     

氟保护漆预防正畸治疗中釉质脱矿的临床研究

目的:探讨氟保护漆在正畸治疗中对釉质脱矿的抑制效果.方法:研究分两组,实验组在粘结托槽时,酸蚀后均匀地涂一薄层氟保护漆,30s后用粘合树脂粘结托槽.对照组粘结托槽时未做其他处理.矫治6个月后,观察两组釉质脱矿情况.结果:实验组的釉质脱矿率明显比对照组低.结论:氟保护漆对正畸治疗中的釉质脱矿有明显抑制作用.     

两种拔牙模式矫治安氏Ⅱ^1类错（牙合）软硬组织变化的对比研究

目的 比较2种拔牙模式矫治安氏Ⅱ1类错牙合前后软硬组织的变化,为临床治疗提供参考.方法 选择下切牙较直立、下颌牙弓轻度拥挤但磨牙严重远中关系,且存在下颌第三磨牙的牙性或轻度骨性的安氏Ⅱ1类错牙 合的患者28例,随机分为A、B2组,其中A组13例只拔除上颌2个第一前磨牙,B组15例拔除上颌2个第一前磨牙、下颌2个第三磨牙.2组均采用方丝弓矫治技术矫治,应用头影测量技术对其矫治前、后软硬组织的变化进行测量,分析其测量结果.结果 2组患者矫治后,前牙均达到正常覆牙合覆盖关系,尖牙达中性、磨牙达完全远中关系.矫治后B组较A组下颌第一磨牙位置平均向远中多移动1.35 mm,鼻唇角平均多增大1.43°,而A组较B组L1/MP平均多增大2.37°,下切牙凸距平均多增大0.79 mm,2组比较,差异均有统计学意义(均P＜0.05).结论 2种拔牙模式矫治这类安氏Ⅱ1类错牙 合,其软、硬组织均能得到一定程度的改善,但拔除上颌2个第一前磨牙和下颌2个第三磨牙矫治后,下前牙仍保持直立,上前牙得到更多的内收,侧貌改善更趋向协调.     

Van.Beek功能矫治器治疗安氏Ⅱ’类早期骨性错的研究

目的 ,对 Van.Beek功能矫正器治疗安氏 类早期骨性错的效果进行观察。方法 :应用 Van.Beek功能矫正器对 12名 10 - 12岁安氏 类骨性错患者进行治疗 ,以 X线头影测量分析方法评价疗效。结果 :上下颌骨矢状不调减小 ,Van.Beek功能矫正器能在水平方向产生抑制上颌骨生长 ,刺激下颌骨生长 ;在垂直方向控制上下颌骨后下旋转 ,压低上下前牙及伸长下磨牙的效果。结果 :Van.Beek功能矫正器治疗安氏 类早期骨性错 ,可产生显著的生长改良作用。     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

高效液相色谱法测定乳酸环丙沙星注射液的含量

乳酸环丙沙星是第三代喹诺酮类抗菌药物,其剂型有注射液.文献报道用比色法、紫外分光光度法、高效液相色谱法测定盐酸环丙沙星的含量 [1],用吸收系数法测定乳酸环丙沙星注射液的含量 [2],用高效液相色谱法测定环丙沙星在人血清中的药物浓度 [3].本文建立了高效液相色谱法测定乳酸环丙沙星注射液的含量,方法简单,重现性好.     

悬浮法培养C6胶质瘤细胞系和该细胞系中脑肿瘤干细胞的分离

目的研究应用无血清培养基悬浮法培养C6胶质瘤细胞系的方法;并分离该细胞系中的脑肿瘤干细胞,观察其生长方式和分化特征.方法应用培养大鼠神经干细胞的培养基和培养方法,在无血清培养基中采用悬浮法培养大鼠C6胶质瘤细胞系;再将分离获得的悬浮生长的脑肿瘤干细胞接种于含血清培养基,观察其分化;应用有限稀释和单克隆形成实验确定该细胞系中肿瘤干细胞的比例.结果C6胶质瘤细胞系中有约1.18%的肿瘤细胞在无血清培养基中能够存活、增殖,形成自由漂浮的细胞球;这种细胞球具有人脑肿瘤干细胞球的特征,并可连续传代,若重新接种于含血清培养基中可重新贴壁分化,贴壁分化后细胞形态与直接在含血清培养基中培养的C6胶质瘤细胞系无明显差别.结论C6大鼠胶质瘤细胞系中含有比例较低的、具有增殖和分化能力脑肿瘤干细胞(约1.18%),该细胞系可在含生长因子的无血清培养基中悬浮生长,并维持细胞系.