Myocardial protection of immature rabbits with an ATP- sensitive K+ channel opener pinacidil

Objective To investigate the effectiveness of pinacidil, an opener of ATP- sensitive K(+) channels, in protecting the myocardium of immature rabbit hearts from ischemic r eperfusion injury. Methods Rabbit hearts underwent 30 min of global normothermic ischemia followed by 30 mi n of reperfusion on the modified Langendorff apparatus. Fifty- two isolated hea rts of 3-4 week- old immature rabbits were divided into 4 groups randomly. Duri ng ischemia, 3 different cardioplegic solutions were administered intermittently by infusion every 15 min (20-25 ml each time in all groups). Group 1: con trol group (n=13); group 2: Krebs- Henseleit (K- H) solution with potassium (16 mmo l/L) (n=13); group 3: K- H solution with potassium (16 mmol/L) and pinacidil (5 0 μmol/L) (n=13); group 4: K- H solution with potassium (16 mmol/L), pinacidi l (50 μmol/L) and glibenclamide (10 μmol/L) (n=13). The pre- ischemic and p ost- ischemic myocardial functions were assessed by the percentage recovery of t he left ventricular developed pressure (LVDP); the left ventricular end- diastol ic pressure (LVEDP); both the positive peak and negative peaks of the first deri vative of the left ventricular pressures (±dp/dt(max)); coronary flow; the level of creatine kinase (CK), lactic dehydrogenase (LDH) and aspartate transcar bamoylase (AST) in coronary sinus venous effluent; and by myocardial ultrastruct ural changes. Results Before myocardial ischemia, there were no significant differences among the four groups in any of the parameters mentioned above. Post- ischemic recovery of LV DP, LVEDP, ±dp/dt(max), coronary flow, the level of CK, LDH and AST, and my ocardial ultrastructural changes were better in group 3 than those in the three other groups.Conclusions As a new and effective composition, pinacidil can significantly improve myocardi al protection from cardioplegia for immature rabbit hearts.     

α—淀粉酶对血链球菌粘附程度的影响

目的研究唾液α-淀粉酶与致龋菌血链球菌粘附的关系,为龋病的病因学研究和预防提供理论依据。方法采用不同浓度α-淀粉酶及全唾液作为实验性获得性膜成分,观察经放射性同位素     

表皮生长因子,谷氨酰胺强化的全胃肠外营养对肠屏障功？…

目的 探讨表皮生长因子（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ,ＥＧＦ）、谷氨酰胺（ｇｌｕｔａｍｉｎｅ,ＧＬＮ）对全胃肠外营养（ｔｏｔａｌ ｐａｒｅｎｔｅｒａｌ ｎｕｔｒｉｔｉｏｎ,ＴＰＮ）并发症的防治作用及机制。方法 将大白鼠分为４组：Ａ（正常喂养组）、Ｂ（标准胃肠外营养ＳＴＰＮ）、Ｃ（ＴＰＮ＋ＧＬＮ）、Ｄ（ＴＰＮ＋ＥＧＦ＋ＧＬＮ）,采用大白鼠ＴＰＮ模型,一周后取大鼠近端空肠组织切片行光     

住院医师临床专业技能考核方法与实践

介绍了上海市第一人民医院开展住院医师规范化培训２０年来,在培养住院医师临床思维能力及临床专业技能方法取得一些新的实践经验：重视信院医师临床专业技能培养,不断尝试和改进考核方法,既注重考核内容的实用性,又考虑考核方法的可操作性。     

论如何提高继续医学教育质量

卫生部继续医学教育委员会成立以来,全国继续医学教育工作得到了快速发展。全国各大医学院校和各医疗单位积极申报国家级继续医学教育项目,申报的项目数量逐年增加。从全国各大医学院校和各医疗单位举办项目的情况看,项目执行民政部较好,办班的教学质量较高。但也存在一些问题,如一些申报的项目质量和水平不够高,办学质量有待进一步加强等。鉴于此,就如何提高继续医学教育质量,提出看法,展开论述。     

儿童右胸外侧小切口在心内直视手术中的应用

随着心脏手术安全性的提高以及外科技术的日趋成熟,术后切口美观问题日益受到人们的重视［１］。我院自１９９７年４月～２０００年８月采用右胸外侧及前外侧小切口体外循环下修补心内畸形３２例,效果满意,现报道如下。１．一般资料:本组３２例中男１１例,女２１例;年龄７个月～１６岁,平均５．４２岁;体重７～４９ｋｇ,平均１７．６ｋｇ。心胸比例０．５～０．６５,平均０．５７。手术后诊断为继发孔房间隔缺损(ＡＳＤ)１９例,室间隔缺损(ＶＳＤ)１３例。合并畸形:左上腔静脉(ＬＳＶＣ)４例,右下肺静脉异位引流(ＰＡＰＶＣ)２例,右室流出…     

不同工艺各类棉酚的反相薄层色谱分析

目的：考察苯胺法与酸法工艺制备的各类棉酚的质量,本胺法工艺制品中是否残留本胺棉酚。方法：采用RP18F反相薄层板,以丙酮-水（4：1）为展开系统分离展开。结果：苯胺法工艺生产的棉酚中出现苯胺棉酚的斑点。结论：首次用反相薄层色谱法确证了苯胺法工艺生产的棉酚中残留有苯胶棉酚。     

手背逆行筋膜蒂岛状皮瓣修复手部软组织缺损

目的 :介绍手背逆行筋膜蒂岛状皮瓣修复手部软组织缺损的方法和疗效。方法 :对 11例手部软组织缺损 ,采用手背逆行筋膜蒂岛状皮瓣修复。术中切取皮瓣面积最大 6cm× 4cm ,最小 3cm× 2cm ,术后石膏托固定 2周。结果 :8例完全成活 ,3例皮瓣尖端部分浅表坏死 ,经换药后愈合。结论 :手背逆行筋膜蒂岛状皮瓣的优点是切取皮瓣时不需暴露掌背动脉 ,不切断伸肌腱及腱联合 ,不需牺牲知名主干血管 ,操作简单 ,对手部功能无影响