差示扫描量热法鉴别三层共挤输液用膜初探

目的：初步探索用差示扫描量热法定性鉴别三层共挤输液用膜。方法：采用差示扫描量热法分析三层共挤输液用膜和其生产所用的粒料之间的相关性,并考察其影响因素。结果：三层共挤输液用膜与其粒料的熔融峰和结晶峰基本一致,升温速率和试样量均对其DSC曲线产生影响。结论：差示扫描量热法能定性鉴别三层共挤输液用膜,为其鉴别项目标准修订提供参考。     

RP-HPLC法测定长春西汀葡萄糖注射液中有关物质及含量

目的：建立测定长春西汀葡萄糖注射液中有关物质及含量方法。方法：采用RP-HPLC法,色谱柱C18（4.6 mm ×250 mm,5μm）,以乙腈-0.2 mol· L^-1醋酸铵溶液（60∶40）为流动相,流量1.0 mL· min^-1,检测波长280 nm,柱温30℃,进样量20μL。结果：在选定的色谱条件下,主成分及其有关物质能完全分离,已知杂质与长春西汀的相对响应因子均在0.9～1.1之间;长春西汀在0.0107～0.0305 mg· mL^-1范围内浓度与峰面积的线性关系良好（r＝0.9997）,平均回收率为99.7％,RSD为0.6％（n＝9）,检测限为0.071μg· mL^-1。结论：该法专属性强,准确度高,可用于长春西汀葡萄糖注射液中有关物质及含量的测定。     

Distinct plasmon resonance enhanced microwave absorption of strawberry-like Co/C/Fe/C core–shell hierarchical flowers via engineering the diameter and interparticle spacing of Fe/C nanoparticles

Strawberry-like Co/C/Fe/C core–shell hierarchical flowers (CSHFs) consisting of separated Fe/C nanoparticles (NPs) anchoring on a Co HF surface were prepared by decomposing Fe(CO)₅ in the presence of Co HFs. Changing the decomposition temperature (T_d) and Fe(CO)₅ volume (δ) could also facilely modulate the phase structure, surface morphology and composition of the products. The low T_d and small δ helped form Co/C/Fe/C CSHFs with a strawberry-like plasmon surface. The diameter and interparticle spacing-dependent electromagnetic properties were investigated at 2–18 GHz. The interparticle-spacing-to-diameter ratio determines the plasmon resonance and coupling. The permittivity and permeability enhanced by strong plasmon resonance were exhibited by Co/C/Fe/C CSHFs formed at δ = 3–4 mL with the interparticle-spacing-to-diameter ratio of 1.36–0.76. The collective oscillation of the conduction band electrons and near field on the Co/C and Fe/C surfaces generated a surface plasmon resonance and coupling, which were responsible for significantly enhanced permittivity and permeability with negative values. In view of the synergistic effect of the enhanced permittivity and permeability, dual dielectric relaxations, dual magnetic resonances, high attenuation and good impedance matching, Co/C/Fe/C CSHFs with particle size of 110 ± 20–380 ± 100 nm and interparticle spacing of 150 ± 50 nm were excellent absorbers that feature strong absorption, broad bandwidth and light weight. An optimal reflection loss (R_L) of −45.06 was found at 17.92 GHz for an absorber thickness of 1.6 mm, and the frequency range (R_L ≤ −20 dB, 99% absorption) was over 2–18 GHz. Our findings demonstrated that optimally designed plasmonic heterostructures must be fabricated to improve microwave absorption performances for future applications.

Plasmon resonance enhanced permittivity, permeability, and microwave absorption were found in Fe/C nanoparticles anchoring on Co/C hierarchical flowers synthesized through a carefully devised kinetically tuned procedure.     

老年骨质疏松性椎体压缩骨折并发症的影像学研究

目的 观测探讨老年骨质疏松性椎体压缩骨折并发缺血坏死不愈合的影像学表现。方法45例骨质疏松性压缩骨折并发缺血坏死不愈合病例,20例经X线平片和CT检查,24例仅摄X线片,1例仅作CT检查,对所有X线平片及CT影像进行回顾分析。结果 44例49个椎体的X线平片测量,最大压缩比(病变椎体最薄部位厚度与相邻椎体对应部位厚度均值之比） 为24. 2% ±7. 0%,楔变比为39. 1% ±8. 7%,所有病变椎体的最大压缩比均少于1/3。根据病变椎体X线平片是否见到椎体 “真空现象”,49个椎骨分为两个类型：（1)吸收压缩型,21例25个椎体,主要表现为椎体明显吸收压缩,密度不均匀;（2)假关节不愈合型,24例24个椎体,主要表现为椎体内存在裂隙,骨折不愈合,椎体两部分间有假关节形成。21例经CT扫描患者, 共26个椎体内见到该病变的所谓特征表现“真空现象”,气体多位于椎体裂隙内,其中的11个椎体在X线平片未见明显气体。结论 老年骨质疏松性椎体压缩骨折并发缺血坏死和骨折不愈合,除了椎体内“真空征象”以外,有多项影像学表现对该病症的诊断有重要价值。     

营养透析防治慢性肾功能不全时营养不良的疗效观察

目的：探讨营养透析（ＮＨＤ）对慢性肾功能不全（ＣＲＦ）病人的疗效。 方法：观察３０例ＣＲＦ病人进行ＮＨＤ前、后各营养评价指标的变化，以ＮＨＤ前、后自身对照，数据以比用配对ｔ检验。 结果：ＮＨＤ后病人血ＰＡＢ、ＡＬＢ、ＴＬＣ、Ｈｇｂ及Ｈｃｔ均提高，而血脂质及载脂蛋白水平无明显变化。 结论：营养透析作为防治ＣＲＦ营养不良的新型疗法具有良好的疗效。     

Alzheimer病模型大鼠脑组织c-fos基因的表达

为了研究手术切断穹窿 -海马伞所建立的 Alzheimer病模型大鼠脑组织中原癌基因 c-fos的表达状况 ,于建模后 1h、6d和 15 d对大鼠进行灌注固定 ,取含海马结构的脑组织分别进行 H-E染色和 FOS蛋白的免疫组化反应 ;同时于建模前后对大鼠进行电迷宫检查。结果表明 :FOS样免疫活性在 Alzheimer大鼠的海马及大脑皮层均有增强 ,并随术后时间的延长有不断增强的趋势 ,因而认为这种 Alzheimer模型可以模拟出与 AD患者类似的 c-fos过度表达     

The Effect of Dehydroepiandrosterone on the Expression of AT1 receptor and TNF-induced ICAM-1 in Vascular Smooth Muscle Cells

Objectives To further investigate the molecular mechanism of vasoprotective role of dehydroepiandrosterone (DHEA), we examined DHEA on AT1 receptor and ICAM-1 gene expression in vascular smooth muscle cells (VSMCs). Methods RT-PCR and Western Blot was used to determine the change of the expressions of mRNA and protein of AT1 and ICAM-1 when given various concentration dehydroepiandrosterone. Results 1.AT1 was abundant under the basal condition. The expression of AT1 mRNA and protein decreased after stimulated by DHEA (at 10^-10mol/L, 10^-8mol/L, 10^-6mol/L), and the effects of DHEA on AT1 protein was dose-dependent. ER inhibitor Tamoxifen and AR inhibitor Flutamide enhanced AT1 protein expression, but did not influence the mRNA expression. 2. The exp-ression of ICAM-1 gene was low under the basal condition.It increased when induced by TNF-α, but decreased when induced by DHEA (at 10^-10mol/L, 10^-8moL/L, 10^-6mol/L) ,and the effects of DHEA on ICAM-1 gene expression were dose-dependent. Conclusions These findings suggest that DHEA modulates AT1 and inflammatory factor induced ICAM-1 gene expression in VSMC, butfurther studies are necessary in the mecha-nism of DHEA action.     

miRNA对秀丽隐杆线虫寿命的影响

miRNA具有调控基因表达的作用,目前关于miRNA的发育和作用机制已经有了相对深入的研究。近些年来miRNA对寿命的影响正逐渐被人们所重视,对其作用靶点的进一步研究有利于治疗衰老相关疾病。     

MicroRNA-335 inhibits invasion and metastasis of colorectal cancer by targeting ZEB2

To determine whether chemotherapy treatment at least 6 months prior to the detection of hepatic steatosis is associated with advanced hepatic fibrosis. Demographics, comorbid conditions, and laboratory data for cancer patients with hepatic steatosis were reviewed. The primary end point of this study was a low probability of fibrosis as calculated by the AST-to-platelet ratio index (APRI)—a surrogate for the absence of histologic bridging fibrosis and/or cirrhosis. Of 279 patients, 117 (41.9 %) were treated with chemotherapy and 197 (66.3 %) had a low probability of fibrosis by APRI. A smaller proportion of patients treated with chemotherapy had a low probability of hepatic fibrosis compared with untreated patients (64.1 vs. 75.3 %, p = 0.04). On multivariable analysis, chemotherapy treatment was a negative predictive factor for a low probability of fibrosis (OR 0.366 [95 % CI 0.184–0.708], p < 0.01). Among chemotherapy-treated patients, 75 (64.1 %) had a low probability of fibrosis. There were no differences in chemotherapy duration (mean 7.8 vs. 7.5 cycles) and interval from last dose to steatosis diagnosis (24.3 vs. 21.4 months) between patients with and without a low probability of fibrosis. A smaller proportion of patients treated with irinotecan or 5-fluorouracil had a low probability of fibrosis (37.3 vs. 66.7 %, p = 0.04). On multivariable analysis, irinotecan or 5-fluorouracil treatment was a negative predictive factor for low probability of fibrosis (OR 0.277 [95 % CI 0.091–0.779], p = 0.02). Prior chemotherapy treatment, especially with 5-fluorouracil or irinotecan, is a negative predictor for the absence of advanced hepatic fibrosis among patients with steatosis.     

美国FDA“动物（效应）法规”在生物防御药物开发中的应用及启示

目的： 在应对重大公共卫生事件中，为我国相关生物防御药物的上市审批和应用储备提供参考依据和解决思路。方法： 结合审批实例，对美国FDA"动物（效应）法规"的主要内容、应用范围和实施状况进行综述，针对美国和我国现阶段应对重大公共卫生事件的药品审批机制进行讨论。结果与结论： 在针对可能发生的生物恐怖袭击事件及重大疫情开展的药物研发中，评价尺度可以与普通药物有所区别，应视情做好相应生物防御药物的战略储备，为应对突发重大公共卫生事件提供系统性支持。