医院各区域内空气消毒效果及监测方法的探讨

目的探讨医院各区域内空气消毒效果及监测、培养所需时间.方法对3496份消毒后空气采用"平板暴露法”进行监测.第一组848份采样时平板暴露15min、置35C温箱培养48h,第二组2648份采样平板暴露5min、培养24h.结果第一组检测无菌生长率为2.6%、阳性检出率为97.4%、合格率为83.3%;第二组无菌生长率为l7.5%.阳性检出率为82.5%,合格率为88.4%.结论根据尘粒(1～10μ粒径)自然下降规律和所带细菌受损后修复需要时间,我们认为采用"平板暴露法”监测消毒后空气时以暴露15min培养48h为好.     

The effects of ropivacaine on sodium currents in dorsal horn neurons of neonatal rats

We used a whole cell patch clamp technique to study the effects of ropivacaine on rat dorsal horn neurons. Under voltage clamp, ropivacaine (10-400 microM) produced a dose-dependent inhibition of sodium current. From a holding potential (V(h)) of -80 mV, sodium currents evoked by test pulses to 0 mV were inhibited by ropivacaine with a mean drug concentration required to produce 50% current inhibition (IC(50)) value of 117.3 microM, which was more than the value of the bupivacaine (IC(50) 53.7 microM). The inhibition effect of ropivacaine was also voltage-dependent. Current evoked from a V(h) of -60 mV was inhibited by ropivacaine with a mean IC(50) value of 74.3 microM, which was less than that obtained at the V(h) of -80 mV. The inhibition effect of ropivacaine on sodium current was use dependent. Repeated activation by a train of depolarizing pulses (5 Hz, 20 ms) increased the inhibitory effects of ropivacaine. The ratio amplitudes of the 20th to the first pulse were 91.2% and 71.1%, respectively, in the absence and presence of ropivacaine (50 microM). Ropivacaine also produced a significant hyperpolarizing shift of 11 mV in the steady-state inactivation curve of sodium current. The inhibition of ropivacaine on the sodium channel may contribute to the mechanism of action of local anesthetics during epidural and spinal anesthesia.     

充分发挥思想政治工作在医院建设中的作用

思想政治工作，在医院发展建设中具有极其重要的作用。我院发挥思想政治工作的作用，在转变职工思想观念、推进医院文化建设、关心职工生活、提高职工综合素质、调动职工积极性，为医院改革发展提供思想保证等方面，做了大量尝试和探索，形成了独具特色的基本经验，多次荣获省、地“思想政治┄┄┄┄     

金莲花软胶囊中总黄酮的含量测定

目的建立紫外法测定金莲花软胶囊中总黄酮的含量测定方法。方法采用紫外分光光度计,检测波长500nm,以芦丁为标准品测定本品总黄酮含量。结果测得线性回归方程为y=0.11359x-0.0034,r=0.9999(n=5),平均回收率为99.67%,RSD为0.58%(n=9)。结论本方法简便,准确、重复性好。     

化疗增敏剂影响 P-糖蛋白依赖多药耐药性肿瘤细胞~(99)Tc~m-MIBI 摄取的实验研究(英文)

目的观察化疔增敏剂对多药耐药性(Multi-drug resistance,MDR)肿瘤细胞 P-糖蛋白(P-glycoprotein,P-gp)的影响及伴随~(99)Tc~m-MI-BI 摄取动力学的变化,以建立用~(99)Tc~m-MIBI 来评价化疗增敏剂效果的方法。方法 MDR 人乳腺癌细胞 MCF-7/Adr 37℃培养。(1)实验组和对照组细胞培养基中分别加入化疗增敏剂维拉帕米(10 μmol/L)和等体积培养液 DMEM。~(99)Tc~m-MIBI 与细胞共同孵育2 h 后收集细胞,测定放射性活度和 P-gp 表达水平。(2)维拉帕米(10 μmol/L)加入细胞培养基中,与细胞孵育若干时间后~(99)Tc~m-MIBI 加入细胞培养基中,与细胞一起培养2 h 后收集细胞。测定放射性活度和P-gp 表达水平。结果 (1)维拉帕米作用2h 后,细胞摄取~(99)Tc~m-MIBI 较对照组显著增加(t=2.33,P<0.05),但 P-gp 表达水平差异无显著性(P>0.05)。(2)肿瘤细胞摄取~(99)Tc~m-MIBI 随维拉帕米作用时间的延长而增加,各时间点 UR 差异有显著性(F=58.2,P<0.05),并且,VRP 作用时间在80 min 内时,肿瘤细胞摄取~(99)Tc~m-MIBI 与 P-gp 的表达水平无相关性(r=0.16,P>0.05),VRP 作用时间大于8 h时,肿瘤细胞摄取~(99)Tc~m-MIBI 与 P-gp 的表达水平呈负相关(r=-0.73,P<0.01)。结论化疗增敏剂能够影响 P-gp 过度表达所致 MDR 肿瘤细胞对~(99)Tc~m-MIBI 的摄取。     

Quantifying the effects of anesthesia on intracellular oxygen via low-cost portable microscopy using dual-emissive nanoparticles

Intracellular oxygenation is an important parameter for numerous biological studies. While there are a variety of methods available for acquiring in vivo measurements of oxygenation in animal models, most are dependent on indirect oxygen measurements, restraints, or anesthetization. A portable microscope system using a Raspberry Pi computer and Pi Camera was developed for attaching to murine dorsal window chambers. Dual-emissive boron nanoparticles were used as an oxygen-sensing probe while mice were imaged in awake and anesthetized states. The portable microscope system avoids altered in vivo measurements due to anesthesia or restraints while enabling increased continual acquisition durations.     

护理风险预警机制在慢性乙型病毒性肝炎肝衰竭病人护理中的应用效果

目的:探讨护理风险预警机制在慢性乙型肝炎肝衰竭病人护理中的应用效果。方法:选取2018年1月—2019年12月我院收治的104例慢性乙型肝炎肝衰竭病人,按照病人入院顺序,并采用单双号抽签方式分为对照组和观察组,各52例。对照组给予常规护理干预,观察组临床护理中引入护理风险预警机制,对比两组肝功能指标、并发症发生情况、护理满意度、护理质量评分、护理缺陷评分及住院时间。结果:干预后,两组肝功能指标均有所改善,且观察组下降幅度较对照组大(P<0.001);观察组与对照组并发症发生率分别为7.69%、25.00%,观察组与对照组总满意度分别为96.15%、76.92%,观察组病人护理质量评分高于对照组,护理缺陷评分及住院时间低于对照组,差异均有统计学意义(P<0.05)。结论:将护理风险预警机制用于慢性乙型肝炎肝衰竭病人的临床护理中,有利于改善病人肝功能指标,减少并发症及护理缺陷,提高护理满意度,提升护理质量,缩短病人住院时间。     

新型冠状病毒感染相关急性黄斑区神经视网膜病变的神经眼科特征

目的：总结新型冠状病毒(SARS-CoV-2)感染相关急性黄斑区神经视网膜病变(AMN)的神经眼科特征。

方法：纳入2022-12-27/2023-02-01就诊于西安市第一医院(西北大学附属第一医院)神经眼科并经相关检查确诊为AMN的患者8例(6例女性,2例男性)共14眼纳入本次研究。所有患者病前均有SARS-CoV-2感染史。收集患者的临床资料。纳入最佳矫正视力(BCVA)、非接触间接眼压测定、眼底彩色照相、近红外成像(IR)、频域光学相干断层扫描(SD-OCT)、OCT血流成像(OCTA)、眼底荧光素血管造影(FFA)、吲哚菁绿血管造影(ICGA)、视野、视觉诱发电位(VEP)和视网膜电流图(ERG)检查结果。对纳入患者的神经眼科特征进行分析总结。

结果：纳入的8例患者年龄20-43(平均30±6.63)岁。病前3-11(平均5±3.51)d有SARS-CoV-2感染史,8例患者中有6例在感染SARS-CoV-2的5 d内出现视觉症状。患者的临床表现为视力下降或视物遮挡感; 纳入患者的视力0.08-1.0; 视野缺损表现为中心、旁中心暗点或合并周边暗点。VEP可见P100或P2峰时延迟; ERG提示视网膜感光细胞 功能受损。在疾病早期,SARS-CoV-2感染相关AMN患者可能存在视力、视野和视网膜外层病变大小及形状可能不匹配的情况,并发现视力越低,VEP峰时越迟。

结论：除眼底多模影像,SARS-CoV-2感染相关AMN的神经眼科特征需要临床医师关注,视功能及电生理可以辅助临床更加全面评估患者及判断预后。     

曾被误诊的麻痹性痴呆23例分析

目的:研究麻痹性痴呆的诊断与治疗。方法:对曾被误诊的23例麻痹性痴呆,使用简明精神病评定量表(BPRS)及简易精神状态检查(MMSE)评定。并予驱梅毒及抗精神病药治疗。结果:驱梅毒治疗后可显著提高疗效。结论:麻痹性痴呆应及早正确诊断,作驱梅毒及抗精神病治疗。     

Treatment of 40 cases of infantile convulsion by Tuina

Infantile convulsion is a common disease in infants and babies and often caused by scare and improper nursing. Its main clinical manifestations are fright,crying and waking up with a start, etc. Since 1998, we have treated 40 cases by Tuina. It is summarized as follows.