黄振翘治疗再生障碍性贫血的经验

黄振翘教授擅长运用中医药治疗再生障碍性贫血等疑难血液病 ,认为再障治疗宜掌握标本缓急 ;注重补肾泻肝 ;扶正不忘祛邪 ;强调整体治疗 ,临床取得了良好疗效。     

Influence of moxa smoke on mitochondrial transmembrane potential and Bax/Bcl-2 in alveolar type II epithelial A549 cells

Objective

To investigate the influence of moxibustion products on mitochondrial transmembrane potential (MTP) and mRNA expression of Bax/Bcl-2 in alveolar type II epithelial A549 cells, and to further explore influence of moxibustion products on the oxidative damage of A549 cells.

Methods

Smoke and particles generated by moxibustion were collected using the filter box for gas sampling. The moxa smoke extract (MSE) was diluted sequentially to the final concentrations of 0.05 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL using the cell culture medium, and A549 cells were then intervened by the above MSE solution. Cell MTP was detected by JC-1 staining. Fluorescence quantitative polymerase chain reaction (PCR) was used to detect Bax/Bcl-2 mRNA expression of A549 cells.

Results

Compared with cells in the normal control group, MTP was significantly decreased in cells of 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.01); while MTP showed no significant changes in cells of 0.05 mg/mL, 0.1 mg/mL and 0.2 mg/mL MSE intervention groups (P>0.05); compared with cells in 0.05 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.05 ); compared with cells in 0.1 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.4 mg/mL MSE intervention group (P<0.01). Bax mRNA expression of cells in each concentration of MSE intervention group all showed no significant difference compared to that in the normal control group; Bcl-2 mRNA expression of cells was reduced with the increase of MSE intervention concentration. Wherein, Bcl-2 mRNA expressions of cells in 0.4 mg/mL and 0.3 mg/mL MSE intervention groups were significantly reduced compared with that of cells in the normal control group (P<0.05); Bcl-2 mRNA expression of cells in 0.4 mg/mL MSE intervention group was significantly reduced compared to that in 0.05 mg/mL MSE intervention group (P<0.05).

Conclusion

Certain higher concentration of moxa smoke could reduce MTP and mRNA expression of the anti-apoptosis gene Bcl-2 in alveolar type II epithelial A549 cells. Oxidative damage may be the important mechanism of apoptosis caused by the high concentration of moxa smoke solution, and further studies are necessary on the specific mechanisms.

     

慢性ITP外周血单个核细胞Th1/Th2亚群反应状态及生血灵干预研究

目的探讨辅助性T淋巴细胞亚群Th1、Th2在慢性特发性血小板减少性紫癜(ITP)发病中的作用,观察生血灵对慢性ITP Th1、Th2亚群功能状态的影响。方法以酶联免疫吸附法(ELISA法)检测慢性ITP患者外周血单个核细胞(PBMC)培养上清中Th1型细胞因子IL-2和Th2型细胞因子IL-10的水平。结果慢性ITP患者PBMC培养上清中IL-2和IL-10的含量均较正常对照组明显减低(P均<0.001),生血灵对其具有明显提升作用(P<0.01)。结论慢性ITP患者Th亚群免疫功能低下。生血灵治疗慢性ITP能够增强Th亚群免疫功能,恢复其平衡。     

人参多糖注射液对环磷酰胺增效减毒作用实验研究

目的：研究人参多糖注射液对肿瘤的抑制作用及其对免疫功能的影响,并探讨其对环磷酰胺（CTX）化疗的增效减毒作用。方法：以S180瘤株ICR小鼠作为病理模型,随机分为模型组、未造模组、CTX组、联合用药组。连续给药10天后,测定其抑瘤率、白细胞数、胸腺指数和脾指数。同时复制CTX 致免疫低下小鼠模型,考察人参多糖注射液对血清溶血素和单核细胞吞噬功能的影响。结果：人参多糖注射液和CTX 合用时,能显著提高抑瘤率;同时减轻CTX 的毒副作用;对CTX 致免疫低下功能有一定的改善功能。结论：人参多糖注射液对CTX 具有明显的增效减毒作用。     

基于数学思维构建中药“性-效关联”模型

本研究尝试以数学关系式来描述中药药性与功效之间的关联,并建立数学模型。在此基础上,运用统计学进一步分析,总结中药的性-效关联规律。力求对药性理论进行现代阐释的同时,增加药性理论的确定性。     

桂枝汤对体温双向调节作用机理探讨——对发热及低体温大鼠下丘脑PGE_2含量及COX活性的影响

目的 :研究桂枝汤对体温双向调节作用与下丘脑PGE2 含量及COX活性的关系。方法 :采用放免法对下丘脑中PGE2 含量及COX活性进行同步检测。结果 :在酵母诱导发热大鼠中 ,桂枝汤可使下丘脑中PGE2 含量明显降低 (P <0 .0 5) ,而在安痛定诱导的低体温大鼠中 ,它可使PGE2 含量明显增高 (P <0 .0 5)。在两种模型中 ,桂枝汤对下丘脑细胞中COX活性的影响均不明显 (P >0 .0 5)。结论 :桂枝汤对体温的双向调节作用可能部分是通过影响PGE2 含量来实现的 ,但PGE2 含量增减不依赖于下丘脑细胞中COX活性的变化。     

益气养阴清热解毒法对EB病毒感染者中医体质证候的影响

目的:观察益气养阴清热解毒法对EB病毒感染者中医体质证候的影响。方法:对EB病毒感染者44例行随机对照试验。治疗组服用益气养阴清热解毒方药,连续服3个月;对照组未服任何药物,3个月后复查。比较两组观察前后体质证候平均积分及EBV-VCA/IgA滴度的变化。结果:治疗组的虚弱体质证候平均积分从治疗前2.54±2.11,降为治疗后1.19±1.29,差异有统计学意义(P<0.05);而其它体质证候平均积分在治疗前后差异无统计学意义(P>0.05)。对照组观察前后各体质证候平均积分差异无统计学意义(P>0.05)。治疗组治愈率58.62%,有效率20.69%,总有效率79.31%,对照组治愈率13.33%,有效率6.67%,总有效率20.00%(P<0.01)。结论:益气养阴清热解毒法能改善EB病毒感染者的虚弱体质证候,降低EB病毒活性。     

四瓣技术在改良结膜囊泪囊鼻腔吻合术中的应用

目的:探讨四瓣技术在改良结膜囊泪囊鼻腔吻合术中应用的安全性及有效性。方法:回顾性临床研究。选取2017-09/2020-06在我院就诊的泪道疾病患者28例28眼作为研究对象,均行结膜囊泪囊鼻腔吻合术。术后随访6～12mo,观察患者手术效果、满意度及术后并发症情况。结果:本组患者术后荧光素钠染料排泄试验阳性率为96%(27/28),手术成功率为96%(27/28),其中治愈率为68%(19/28),好转率为28%(8/28),无效率为4%(1/28),患者总体满意度为93%(26/28)。术后并发症包括眼部异物感(28眼)、球结膜充血(11眼)、鼻腔结膜囊渗血(8眼)及泪道引流管脱出(1眼)。结论:四瓣技术用于改良结膜囊泪囊鼻腔吻合术手术成功率高,并发症少,安全有效。