老年腹股沟疝无张力修补术与传统修补术的比较

目的 比较无张力腹股沟疝修补术与传统腹股沟疝修补术在老年患者中应用的临床结果、术后生活质量和经济开支。方法 自2000～2001年60岁以上的老年患者42例行腹股沟疝无张力修补术与42例行传统腹股沟疝修补术者，分类比较。结果　无张力腹股沟疝修补术对于老年患者在复发率、术后镇痛、手术时间和术后下地时间方面均明显优于传统腹股沟疝修补术。结论　无张力腹股沟疝修补术运用在老年患者中，近远期效果均优于传统修补手术。     

Ilizarov牵伸技术在外伤性垂足治疗中的应用

[目的]探讨应用Ilizarov牵拉技术治疗外伤性垂足的疗效。[方法]自2004年12月~2006年2月采用Ilizarov技术治疗8例外伤性垂足患者,其中男5例,女3例;年龄28~52岁,平均34.6岁。垂足产生原因小腿胫前外侧肌群断裂或缺损3例,小腿骨筋膜室综合征2例,腓总神经损伤2例,股骨髁上骨折并腘动脉断裂1例。踝关节强直范围为跖屈50°~65°。术中将准备好的牵拉架套入小腿及足部的相应位置。在钢环对应平面交叉穿入2mm克氏针并固定于Ilizarov牵拉架上,术后3~5d开始以1~2圈/d,4次/d的速度旋转踝关节前、后方的调节螺纹杆,使足环带动患足逐渐背伸,使垂足畸形逐渐得以纠正。当垂足提拉达到或超过中立位后停止牵拉,但继续使用牵拉架固定2~3个月以巩固疗效。[结果]所有患者均获随访,随访时间为10~21个月,平均12.5个月。患足均矫正至中立位,有3例患者踝关节可主动背伸至10°,行走功能均良好。有1例患者1个针道发生感染,经输用抗生素,加强针道护理,保证针道引流通畅,感染逐渐控制。无1例发生皮肤坏死、神经、血管损伤等并发症。全部患者垂足畸形无复发。[结论]应用Ilizarov牵拉技术治疗外伤性垂足较以往的治疗方法有明显的先进性,值得推广。     

3种常用化疗药对正常大鼠血清胃泌素及D-木糖醇浓度的影响

目的：观察3种常用化疗药对正常大鼠血清胃泌素及D-木糖醇浓度的影响。方法：将动物随机分为4组，即对照组、ETX组、5-Fu组和THP组，检测胃泌素及D-木糖醇浓度等指标。结果：3组化疗组上述指标与对照组比较，差异有统计学意义（P〈0．05，P〈0．01）。结论：正常大鼠用ETX、5-Fu和THP，血清胃泌素及D-木糖醇浓度明显减低，从临床表现、胃肠功能方面提示3种常用化疗药可导致正常大鼠脾虚证的发生。     

单丝聚丙烯合成非吸收齿状线在美容整形应用中并发症的防治及应用改进

目的探讨单丝聚丙烯合成非吸收齿状线在美容整形中的并发症产生的原因及应用方法及技术的改进。方法对近两年的单丝聚丙烯合成非吸收齿状线在美容整形中的应用进行总结与分析。结果改进应用方法后，并发症明显减少，效果加强。结论单丝聚丙烯合成非吸收齿状线在美容整形中应用得当，可减少并发症、取得显著效果。     

Treatment of severe subglottic laryngotracheal stenosis using hyoid graft with sternohyoid muscle flap]

OBJECTIVE: To evaluate the possibility and reliability of the hyoid-sternohyoid graft transfer in the correction of server subglottic laryngotracheal stenosis, and delineate the operation skills and clinical results. METHODS: Seven patients with severe subglottic stenosis underwent laryngotracheal reconstruction using the hyoid grafts with sternohyoid muscle flaps (HG-SHMF). Five of these patients had traumatic subglottic stenosis, one with scar tissue of unknown etiology arising in the subglottic region, another with tracheal narrowing caused by inhalation of hydrochloric acid. RESULTS: All seven patients were successfully decannulated with moderate good voice. The average time from reconstruction to decannulation was 15.4 months. The stent was endoscopically removed with a range of 3 to 22 months; the mean time required for stenting was 9.6 months. Two patients who received additional salvage reconstruction procedures because of graft or stent displacement were extubated with improved voices and satisfactory airway. CONCLUSIONS: The HG-SHMF transfer was a single-stage reconstruction, relatively simple procedure that can restore an adequate airway and a good voice. Patients undergoing laryngotracheal reconstruction with HG-SHMF must have regular, long-term follow-up since graft displacement and recurrent granulation tissue or scar reformation can cause restenosis after an initially successful surgery. This procedure should be used in a large number of patients to further test its reliability.     

The study of diethylstilbestrol toxic effect in the mouse sertoli cell line by comparison of miRNA and mRNA expression

It is important to acknowledge the harmful effects of environmental chemicals in human’s lives. The toxic effects of Diethylstilbestrol (DES), one of the endocrine-disrupting chemicals (EDCs), have been documented in many studies. As expected, DES affect male gendal hormone as well as female’s; therefore, epigenetic study should be considered. In this study, microarray technology was used to study harmful effects on the level of genomics, and here, two types of microarray chips- the Agilent mouse genome 4 × 44 K array for gene expression profiling and the Agilent mouse miRNA v13 for miRNA expression profiling-was used to study the relation between gene and miRNA expression profiles. As a result, we identified 4 miRNAs (miR 203, 350, 421, and 466i) that were similarly expressed at 3 hrs and 24 hrs of DES treat times. Twenty one genes matched between predicted target for 4 miRNAs and 118 genes expressed similarly. These genes have functions related to cell differentiation and cell cycle. Therefore, DES affects cellular function and induces toxicity in TM4 cells. In future studies, it is necessary to find more related functions and mechanisms of DES in the system.     

护理科研设计应重视人性化

护理科学研究的对象是人，并且是一批特殊的人群，他们不但承受着躯体的痛苦，而且存在多种心理健康问题。护理科研设计是护理科学研究中非常重要的环节，是对科学研究具体内容和方法的设想和安排，所以护理科研设计应突出人性化，以人为本，以减轻病人痛苦、促进早日康复为目的。     

青光眼双滤过手术的临床报告

目的　观察双滤过手术治疗青光眼的疗效。方法　选择 130只眼晚期、绝对期原发性青光眼随机分为两组 ,每组 6 5只眼。 组行双滤过手术 , 组行小梁切除术。结果　术后随访观察 5～ 2 1月 ,平均 (14 .34±4 .35 )个月。 组眼压≤ 2 1m m Hg(1m m Hg=0 .133k Pa) 6 2只眼 (95 .4 % ) ,其中需局部用药 6只眼 (9.2 % ) ,术后平均眼压为 (19.37± 2 .92 ) m m Hg,较术前下降 (33.2 6± 12 .33) m m Hg; 组眼压≤ 2 1mm Hg5 5只眼 (84 .6 % ) ,其中需局部用药 12只眼 (18.5 % ) ,术后平均眼压 (2 2 .6 2± 5 .6 8) m m Hg,较术前下降 (2 7.83± 8.81) m m Hg。两组手术成功率差异有显著性 (P <0 .0 5 )。并发症 : 组 9只眼 (13.8% ) ,其中浅前房 6只眼 (9.2 % ) ,前房积血 3只眼 (4 .6 % ) ; 组 2 2只眼 (33.8% ) ,其中浅前房 15只眼 (2 3.1% ) ,恶性青光眼 2只眼 (3.1% ) ,脉络膜脱离 2只眼 (3.1% ) ,前房积血 3只眼 (4 .6 % )。两组并发症的发生率差异有显著性 (P <0 .0 5 )。结论　青光眼双滤过手术效果好 ,并发症少 ,是治疗青光眼安全有效的手术方法     

Dynamic observations on NK cell activity and IL-2 and IFNγ productionin vitro in patients on maintenance hemodialysis

Summary In order to study the immune function of patients on maintenance hemodialysis (MHD), we assayed NK cell cytotoxicity against K⁵⁶² targets in 40 patients on MHD, and the production of IL-2 and IFN in peripheral blood mononuclear cells (PBMC) after PHA stimulation, in contrast to those in normal controls. The results showed that NK cell activity and IL-2 and IFN levels were markedly lower in the patients than in the controls. Afeter a single dialysis, NK cell activity as well as IL-2 and IFN levels were elevated to different extent. But there was no significant change in patients after long-term dialysis. There was a positive correlation between the NK cell activity and IL-2 and IFN activity in the controls, but no such correlation was found in the patients on MHD. There was a positive correlation between the NK cell activity and IL-2 activity in patients after dialysis, suggesting that immune function were impaired in the patients on MHD, with a decline in the activity of NK cell and IL-2 and IFN, and a disorder of immune regulation cycle. These abnormal immune impairments in the patients could be partly corrected by hemodialysis. However, long-term hemodialysis is not much helpful in the improvement of patient’s immune function.