不同排牙方法对全口义齿咀嚼效能的影响

目的:探讨不同排牙法的全口义齿咀嚼效能的异同。方法:采用上颌排牙法、下颌排牙法和综合排牙法为10名无牙颌患者分别制作3副全口义齿。戴用3个月后,通过吸光度法测定咀嚼效率,比较不同排牙法的全口义齿咀嚼效能的异同,并与有牙颌进行对比。结果:在单侧咀嚼和双侧咀嚼2种咀嚼方式下,全口义齿患者的咀嚼次数和咀嚼效能均少于有牙颌组,本实验3组全口义齿在3个月后均发挥了良好的咀嚼效能。下颌排牙法和综合排牙法全口义齿的咀嚼次数和咀嚼效能无统计学差异,但均好于上颌排牙法全口义齿。结论:下颌排牙法和综合排牙法的全口义齿排列的人工牙能够减少在咀嚼过程中不利于全口义齿稳定因素的影响,提高咀嚼效能。     

唇腭裂患者牙槽嵴数字化模型建立及分析的初步探讨

目的 通过三维激光扫描建立唇腭裂患者牙槽嵴的数字化模型，为唇腭裂患者的模型分析建立科学方法。方法 唇裂修复术前于全麻状态下对1例右侧完全性唇腭裂患者的上颌无牙颌制取模型，用激光扫描仪扫描，通过计算机逆向工程软件进行模型重建。结果 此法可在重建模型上设定测量参考点9个，并可测出各标志点间的距离；模型可切割后测量和计算。结论 激光扫描三维分析方法是分析唇腭裂患者牙槽嵴形态及方向改变的一种理想方法，应用此模型可量化分析唇腭裂患者随生长发育而变化的上颌牙槽嵴形态。     

Consensus conference on immediate loading: the single tooth and partial edentulous areas

PURPOSE: A consensus conference was held to determine what the parameters should be for the immediate functional loading of the single-tooth implant restoration and short-span fixed implant-supported bridgework. MATERIALS: Forty-one clinicians and researchers presented cases and situations relating to the topic. A panel then distilled questions that were presented to the audience (430) at large. Answers were gleaned to formulate a consensus. RESULTS: Ten distinct answers evolved that constituted the essence of guidelines for clinicians to be aware of when undertaking immediate loading. These guidelines are contained within the body of the text. CONCLUSIONS: Extreme caution and adherence to a universal generic protocol are suggested for clinicians who are involved with single-tooth and short-span multiple-teeth implant replacements as related to immediate loading as defined within this text.     

游离血管化髂骨瓣：26例回顾分析

该文对应用游离髂骨瓣修复颌骨缺损的病例进行回顾研究，并分析手术并发症。作者共用26块髂骨肌瓣、1块髂骨肌皮瓣修复18例下颌骨和8例上颌骨缺损。1例因微血管再灌注问题而导致手术失败：3例术后行组织瓣抢救，其中2例吻合口血栓形成，重新吻合血管后抢救成功；10例术后出现股外侧皮神经支配区皮肤麻木，3例并发切口疝，1例供区创口裂开，2例并发口鼻瘘，1例颈部创口感染，     

云南省双江县布朗族头面部器官微机测量研究

目的 对云南省双江县邦丙乡布朗族头面部器官进行全面系统地测量研究。方法 采用自行研制的头面部摄相—计算机测量系统，对正常布朗族成人155人进行了头面部器官全面系统的测量，其中男性96人，女性59人。结果 得出了布朗族成年男女两性头面部器官41个项目测量值和17个头面部指数资料。结论 该研究委仅对临床医学和美学研究有重要意义，而且对人类学、民族学和工业等相关学科也有重要的参考价值。     

Induction of dental pulp fibroblast matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 gene expression by interleukin-1alpha and tumor necrosis factor-alpha through a prostaglandin-dependent pathway

Matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) are involved in the degradation of extracellular matrix in many inflammatory diseases. Little is known regarding the expression of these mediators in dental pulp fibroblasts. The effects of proinflammatory cytokines (interleukin (IL)-1alpha and tumor necrosis factor-alpha (TNF-alpha)) and prostaglandin E2 (PGE2) on pulp fibroblast MMP-1 and TIMP-1 gene expression were investigated. Northern hybridization showed that IL-1alpha and TNF-alpha induced significant MMP-1 gene expression, with only little effect on TIMP-1 gene. Exogenous PGE2, however, upregulated TIMP-1 mRNA synthesis but not MMP-1. Concomitant addition of IL-1alpha and PGE2 or TNF-alpha and PGE2 suppressed MMP-1 mRNA production, compared with the groups treated with IL-1alpha or TNF-alpha alone. In contrast, PGE2 enhanced the upregulatory effects of TIMP-1 mRNA by IL-1alpha or TNF-alpha. Furthermore, cytokine stimulation of MMP-1 and TIMP-1 gene expressions can be enhanced or blocked by indomethacin, respectively, and reversed by exogenous PGE2. These results suggested that cytokine-stimulated MMP-1 and TIMP-1 gene expression in dental pulp fibroblasts was mediated, at least in part, by a prostaglandin-dependent pathway. The differential regulation of IL-1alpha or TNF-alpha-induced MMP-1 and TIMP-1 mRNA synthesis, as well as the direct upregulation of TIMP-1 gene expression by PGE2, also implied that prostaglandin may serve as a protective mechanism from excessive tissue breakdown during pulpitis.     

Purification and characterization of a trypsin-like protease from the culture supernatant of Actinobacillus actinomycetemcomitans Y4

We purified and characterized a protease from Actinobacillus actinomycetemcomitans. The protease was isolated from the culture supernatant by sonication in phosphate-buffered 3-[(3 cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). The protease was purified by acetone precipitation, followed by column chromatography with Arginine Sepharose 4B, DEAE Sepharose CL-6B, Sephacryl S-200HR and HiTrap Q. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified protease showed a clear band at approximately 50 kDa. The protease showed trypsin-like activity with hydrolytic activity for the synthetic substrates N alpha-benzoyl-DL-arginine p-nitroanilide (BApNA) and N alpha benzoyl-DL-lysine p-nitroanilide (BLpNA). The activity of the protease was stable at pH 7.0 to approximately 8.0. The activity of the protease was inhibited by leupeptin, phenylmethylsulfonyl fluoride (PMSF), and EDTA, but was not affected by dithiothreitol (DTT), cysteine, 2-mercaptoethanol, pepstatin or soybean trypsin inhibitor. These data suggest that this protease is a serine protease or metallo protease. This enzyme extensively degraded collagen type I and fibronectin.     

Use of biodegradable polylactic acid barrier materials in the treatment of grade II periodontal furcation defects in humans--Part I: A multicenter investigative clinical study.

This study evaluated two bioresorbable polylactic acid barriers (Epi-Guide and Guidor) to determine if design differences were of therapeutic significance in the treatment of Grade II furcation defects in humans. Forty patients with bilaterally matched, Grade II furcation defects in maxillary or mandibular first or second molars were treated in a multicenter study. Comprehensive initial periodontal therapy, followed by defect debridement and root preparation, preceded randomized membrane placement. Data collected from all three investigative centers were pooled and analyzed using an analysis of variance appropriate for a counterbalancing design. Both barrier types produced measurable improvements of clinical probing values. Barrier exposure scores taken through the eighth week postoperative revealed that Epi-Guide was less likely to become exposed than Guidor. The findings of this study, which was conducted over a 12-month period, demonstrated that Epi-Guide and Guidor were comparable as measured by clinical probing determinations.     

The distribution of nitric oxide synthetase in Vcx and its relation with the expression of FOS induced by teeth movement in rats]

It was studied the central role of nitric oxide(NO) during experimental teeth movement and the relation between nitric oxide synthetase (NOS) positive neurons and FOS like immunoreactivity (FLN) with the NADPH-diaphorase histochemistry and immunocytochemical reaction method. Results indicated that NOS positive neurons and FLN showed typical distribution in Vcx and there was some overlap between them. It suggests that NO is involved in the central modulation of the stimulating message of teeth movement, and which further explains the central modulation mechanism of experimental teeth movement in rats.     

IL—1,IL—6,TNF对人TMJ滑膜细胞蛋白合成的影响

目的：研究人ＴＭＪ滑膜细胞（ＳＭＣ）蛋白质合成能力，与其它组织细胞的差异，及细胞因子对这方面的影响。方法：采用体外培养的ＳＭＣ、ＴＧＣ、ＮＭＣ３种细胞及无血清培养液，应用紫外分光光度仪在２８０ｎｍ波长处，对培养上清中的蛋白含量进行测定，并与加用ＩＬ－１、ＩＬ－６、ＴＮＦ后蛋白质合成的量进行对比观察。结果：正常状态下，ＴＧＣ蛋白合成量最高，ＯＤ值达０．１４，而ＳＭＣ最低，为０．０７９。在加用细胞因子作用４８ｈ后，ＳＭＣ蛋白含量达到０．１１７，提高３０％，而ＴＧＣ、ＮＭＣ却下降１０％～３０％。结论：正常状态下ＳＭＣ合成蛋白质成份较其它细胞少，而细胞因子可刺激ＳＭＣ蛋白质合成增加，这些蛋白成份中可能具有影响关节炎症的物质。