检测胞浆抗原用于急性白血病免疫分型

目的:探讨胞浆抗原在急性白血病免疫分型中的临床意义。方法:用流式细胞仪检测42例初治急性白血病患者胞浆抗原CyCD_3,CyCD_(22),CyCD_(79a)和MPO。结果:T—ALL阳性胞浆抗原主要表达在CyCD_3,阳性率占88.9％。B—ALL阳性胞浆抗原主要表达在CyCD_(79a),AML主要表达CyMPO。结论:CyCD_3和CyCD_(79a),及MPO可分别作为T—ALL,B—ALL和AML系列特异性标志,对正确诊断白血病提供非常有价值的依据。     

22例老年重症心力衰竭合并急性呼吸衰竭患者机械通气的临床观察

目的评价老年重症心力衰竭合并急性呼吸衰竭的救治.方法 22例重症心力衰竭患者由于肺部感染而先出现低氧血症(PaO2 46.42±5.55mmHg),继之急性CO2潴留(PaCO2 88.05±18.75mmHg),发生急性Ⅱ型呼吸衰竭. 22例均行气管插管、机械通气支持.结果 4例经上述治疗后好转,拔除气管插管,恢复正常呼吸.18例无法脱离机械通气,于3～28d内死于多脏器衰竭.结论机械通气支持是目前抢救重症心力衰竭合并急性呼吸衰竭患者常用的方法.但对心功能IV级、心力衰竭晚期、全身衰竭者是否进行此治疗有待进一步探讨.     

Estrogen receptors in the external anal sphincter

Inasmuch as anal competence in women is reduced after the age of 50 years, it may be dependent on effects of estrogens. In this study, samples of the external anal sphincter were analyzed for the presence of estrogen receptors and were found to be present at a median concentration of 5.0 fmol per milligram of protein (range, 1.9 to 13) in women (n = 7), and 1.1 fmol per milligram of protein (range, 0 to 3.2) in men (n = 7). These findings are of interest with regard to the treatment of idiopathic anal incontinence.     

三氮唑核苷不同途径给药治疗喘憋性肺炎疗效评价

本文采用三氮唑核苷超声雾化吸入治疗喘憋性肺炎，并与静脉用药比较，结果表明：用药前及用药后末梢血象变化，雾化给药不影响血液系统，而且有助于红细胞、血红蛋白恢复正常，无论在止咳、平喘、肺部罗音及Ｘ线炎症吸收效果均明显优于静脉给药组（Ｐ＜０．０１），提示超声雾化吸入用于治疗呼吸道感染（ＡＲＩ），可以取代静脉途经用药，其疗效快，安全方便，避免静脉用药所引起的不良反应。     

顺丁烯二酸水溶液的热反应动力学

研究了不同温度、浓度条件下，顺丁烯二酸（顺酸）非催化反应网络，即顺酸可生成反丁烯二酸（反酸）和苹果酸，反酸与苹果酸存在可逆反应。建立了该反应网络的动力学模型。测定了不同反应条件下顺酸、反酸和苹果酸浓度随时间变化的规律，据此，动力学参数进行了估值。     

通脉灵对心肌缺血再灌注犬心功能的保护作用

本实验复制２４只杂种犬心肌缺血再灌注损伤病理模型，应用与生理盐水、丹参对比的方法观察通脉灵对血液动力学及心功能的影响。结果表明，通脉灵小剂量组（ＴＭ１Ｇ）、通脉灵大剂量组（ＴＭ２Ｇ）和丹参对照组（ＤＳＧ）的心输出量（ＣＯ），心脏指数（ＣＩ），心搏指数（ＳＩ）、左室内压峰值（ＬＶＳＰ）及左室内压最大变化速率（±ｄｐ／ｄｔｍｓｘ）均明显高于生理盐水对照组（ＮＳＧ）。本实验证明，通脉灵主要是通过保护心肌舒缩功能改善血液动力学指标进而防止缺血再灌注时心功能不全的发生。     

Identification of Leishmania donovani isolates from different kala-azar foci in China by kDNA hybridization.

kDNA sequence homology of Leishmania donovani isolates from three types of kala-azar foci in China were analyzed by using dot and Southern hybridization with biotin- and 32P-labelled probes. The results revealed kDNA sequence heterogeneity among Leishmania donovani isolates from the three kala-azar foci: sequence homology between isolates of hill and desert foci was higher than that between hill and plain foci isolates. The kDNA hybridization technique was also found to be specific and sensitive for direct identification of Leishmania in animal tissues. In a preliminary survey, kDNA hybridization of cutaneous tissue blots of 71 dogs from endemic regions showed a positive rate of 40.8%, and the rate of double positive cases (touch blot hybridization and bone marrow smear) reached 91.3%. The direct identification of Leishmania in tissues by kDNA hybridization seems to be a useful and convenient method for epidemiological study and clinical diagnosis, especially for species/strain characterization.     

Determination of functional kidney volume by single-photon emission computed tomography in patients with insulin-dependent diabetes mellitus.

Functional parenchymal kidney volume was determined by single-photon emission computed tomography (SPECT) for 99mTc-dimercaptosuccinic acid (DMSA) using a rotating gamma camera in phantom experiments and in patients with insulin-dependent diabetes mellitus (IDDM). The results from the patient examinations were corrected according to the phantom studies and were thereafter set in relation to renal haemodynamics, blood pressure, and urinary albumin excretion. Functional parenchymal kidney volume was significantly greater in diabetic patients compared to that of 11 healthy controls (P < 0.003). Urinary albumin excretion was increased and glomerular filtration rate (GFR) per renal parenchymal volume significantly less in patients with a duration of diabetic disease of more than 15 years compared to patients with shorter duration of disease (P < 0.03 and P < 0.05 respectively). Diabetic patients with a GFR of more than 120 ml/min had greater renal parenchymal volume than patients with lower GFR (P < 0.02). Patients with increased GFR, renal plasma flow (RPF), renal blood flow, or filtration fraction had significantly greater functional parenchymal volume than the healthy subjects (P < 0.01 for all comparisons). We conclude that by application of SPECT for DMSA we were able to show that IDDM patients have greater renal parenchymal volumes than healthy subjects. GFR/kidney volume was increased in IDDM patients with a duration of disease of < 15 years compared to patients with long-standing diabetes. The SPECT technique seems suitable for prospective long-term follow-up studies of functional kidney volume in IDDM patients.     

Protective effect of Re-LPS antiserum on experimental multiple system organ failure.

This study examines the possible beneficial effect of Re-LPS (F515) antiserum on experimental multiple system organ failure (MSOF) in rabbits. The results showed that the plasma LPS level was significantly decreased, and it took a shorter period to clear up LPS in experimental than in control rabbits after receiving Re-LPS antiserum. Pretreatment with antiserum can markedly improve the function of the liver, lungs, kidneys, blood and gastrointestinal tract. The MSOF incidence in the group of rabbits receiving immune sera was only 11.2% and the survival rate was raised by about 40.0%. The results suggest that early passive immunotherapy may neutralize gut-derived endotoxin, inhibit endotoxin-induced mediators release and prevent development of severe complications due to sepsis. It is therefore postulated that LPS core antiserum may provide a prophylactic effect on the development of experimental MSOF.