外固定架治疗桡骨远端粉碎性骨折

目的总结桡骨远端粉碎性骨折的外固定架治疗疗效。方法从1995年～2003年采用外固定架治疗桡骨远端粉碎性骨折46例,并进行随访。结果治疗后均随访1年,术后按Dienst评分,优良率为83．3％。结论外固定架固定牢靠,可早期活动腕关节,是治疗桡骨远端粉碎性、开放性骨折的有效方法。     

Crossed immunoelectrophoretic analysis of anti-Salmonella typhi antibodies in sera of typhoid patients and carriers: demonstration of the presence of typhoid-specific antibodies to a non-O, non-H, non-Vi antigen

A veronal buffer extract of Salmonella typhi was used as the reference antigen and its corresponding rabbit antiserum as the reference antibody in crossed immunoelectrophoresis to analyze antibodies in sera obtained from typhoid patients and carriers. Four precipitating antibodies were regularly detected. Three were against antigens common to other gram-negative bacteria and one appeared to be typhoid specific. Of the three common antigens, one (antigen no. 7) formed a precipitin resembling in mobility and morphology the lipopolysaccharide antigen seen in crossed immunoelectrophoresis analysis of other gram-negative bacteria. The other (antigen no. 19) was heat labile and antigenically similar to the reported common antigen of Pseudomonas aeruginosa. The third (antigen no. 14), also heat labile, was present in members of the family Enterobacteriaceae but not the family Pseudomonas. The typhoid-specific precipitating antibody present in sera of most typhoid patients and carriers but not patients infected with nontyphoid salmonella was directed to a heat-labile, non-O, non-H, and non-Vi antigen (antigen no. 28), probably protein in nature.     

腺病毒载体介导的lacZ基因在NG细胞系及大鼠黑质的表达

本实验用标记基因ｌａｃＺ５型重组腺病毒（Ａｄ５ＣＭＶｌａｃＺ）转染培养的ＮＧ细胞系，Ｘ－ｇａｌ染色检测转染效率．在培养的ＮＧ细胞系，当病毒滴度为２×１０８时，转集率达到５０％，当滴度为２×１０９时，转染率达１００％，有较好的量效关系；固定病毒液度为１０１０，培养２～１６ｈ，细胞的转染率随时间延长而提高，有较好的时效关系。将Ａｄ５ＣＭＶｌａｃＺ注射到大鼠黑质部位后，分别于注射后３～１２０ｄ取脑、切片、Ｘ－ｇａｌ染色，发现黑质局部从第７ｄ开始有部分蓝染，第１０ｄ达高峰，注射局部感染率１００％；９０ｄ时开始下降，持续至１２０ｄ；纹状体等其它部位无蓝染．上述结果提示，腺病毒载体介导的标记基因可在培养的神经细胞系和中脑黑质部位高效表达，为进一步开展中枢神经系统退变性疾病尤其是帕金森氏病的基因治疗奠定基础。     

妊娠高血压患者血浆中降钙素基因相关肽的测定及其临床意义

本文测定了３０例正常育龄妇女，２８例正常妊娠以及３６例妊娠征患者血浆中降钙素基因相关肽的水平，结果发现，轻度妊高生与正常妊娠相比，血浆中ＣＧＲＰＫ是有下降但不显著（Ｐ〉０．０５）。中重度妊高征与正常妊娠相比，差异有非常显著性，因此推测，妊娠状态下血浆中降钙素基因相关肽的减少可能是妊娠高血压症产生的重要原因之一。     

可切削渗透陶瓷玻璃料对不同堆积密度氧化铝基体的渗透

探讨了可切削渗透陶瓷 (Infiltration of Machinable- infiltrated- ceramic,MIC)渗透玻璃在不同堆积密度氧化铝基体中的渗透情况及渗透后复合体的颜色表达。在 110 0℃保温 2 h,将玻璃渗透到不同堆积密度的氧化铝基体中 ,分别测得其渗透深度和颜色参数。扫描电镜观察玻璃—氧化铝复合体断面。玻璃渗透深度的平方与氧化铝堆积密度有直线负相关关系 ,最小渗透深度为 3.0 92 m m。复合体的颜色系数与氧化铝堆积密度无相关性。渗透复合体在断裂过程中可见 :裂纹偏转、晶体拔出和穿晶断裂。本实验证实 MIC渗透玻璃的渗透性能达到临床要求。渗透后的复合体颜色稳定 ,强度可靠     

ESWL实际焦点位置的理论和数值分析

从理论和数值模拟两个方面分析了ESWL中的非线性的动力学焦点和实际焦点的位置 ,给出三种典型的动力学焦点的数值结果 ,说明非线性动力学焦点和实际焦点一般不是几何焦点 ,指出正确确定实际焦点在ESWL技术中的重要性。     

Association analyses of DNA methyltransferase-1 (<Emphasis Type="Italic">DNMT1</Emphasis>) polymorphisms with systemic lupus erythematosus

The etiology of systemic lupus erythematosus (SLE) is very complex, and genetic factors appear to play a significant role in susceptibility to SLE, in determining the disease expression, and in the autoantibody profiles of individuals with SLE. DNA methyltransferase-1 (DNMT1) is a major enzyme that determines genomic methylation patterns and both maintains methyltransferase and exhibits de novo DNA methylation activity in vivo. In order to clarify the association of DNMT1 polymorphisms with SLE, we scrutinized the genetic polymorphisms in exons and their boundaries of DNMT1, including the –1,500 bp promoter region, by direct sequencing in 24 Korean individuals. Twenty-nine sequence variants were identified: two in 5UTR, six in exons, and 21 in introns. Eight of these polymorphisms were selected for a larger-scale genotyping (n=680) by considering their allele frequencies, haplotype-tagging status, and linkage disequilibrium coefficiencies (LDs) among polymorphisms. The associations between DNMT1 polymorphisms and the clinical profiles of SLE were analyzed. No significant associations with the risk of SLE were detected. However, further analyses of association with autoantibody production among SLE patients revealed that one nonsynonymous SNP, +14463G>C (V120L) in exon 4, was weakly associated with an increased risk of anti-La antibody production (P=0.04), although the significance could not be retained after correction of multiple tests. The DNMT1 variations and haplotypes clarified in this study would provide valuable information for future genetic studies of other autoimmune diseases.     

Effect of adenosine agonists on guinea-pig isolated trachea

Inflammation Research -     

足筋膜间隙综合征相关肌肉磁刺激运动诱发电位的临床研究

目的:探讨足筋膜间隙综合征患者相关肌肉的磁刺激运动诱发电位(MEP)的特征及其临床意义.方法:对21例确诊为单侧足筋膜间隙综合征患者,在双侧腘窝上缘用磁刺激坐骨神经,同心圆针电极插入双侧足部各筋膜间隙内相应的肌肉中,记录各自MEP的潜伏期及波幅变化,并计算其比值,分析该比值与足筋膜间隙综合征发生的相关性.结果:正常足部各筋膜间隙内相应的肌肉磁刺激MEP先正后负,潜伏期相对恒定,但波幅不够稳定;当间隙内压大于30mmHg时,间隙内相应肌肉MEP潜伏期明显延长,为健侧的(1.9l±0.23)倍.统计学分析显示,筋膜间隙综合征的发生与磁刺激MEP波幅比值降低之间无相关性(P＞0.05),而与相应肌肉MEP潜伏期比值的变化之间存在着明显相关性(P＜0.01).结论:磁刺激MEP作为诊断足筋膜间隙综合征的客观指标具有重要的价值.