全文获取类型
收费全文 | 211篇 |
免费 | 2篇 |
国内免费 | 19篇 |
专业分类
儿科学 | 5篇 |
妇产科学 | 5篇 |
基础医学 | 14篇 |
口腔科学 | 5篇 |
临床医学 | 19篇 |
内科学 | 37篇 |
神经病学 | 1篇 |
特种医学 | 96篇 |
外科学 | 5篇 |
综合类 | 7篇 |
预防医学 | 7篇 |
药学 | 22篇 |
肿瘤学 | 9篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 3篇 |
2012年 | 6篇 |
2011年 | 1篇 |
2010年 | 4篇 |
2009年 | 3篇 |
2008年 | 3篇 |
2007年 | 14篇 |
2006年 | 1篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 10篇 |
1997年 | 21篇 |
1996年 | 11篇 |
1995年 | 9篇 |
1994年 | 13篇 |
1993年 | 9篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 18篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1980年 | 8篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有232条查询结果,搜索用时 15 毫秒
51.
Mai WP 《中国医药工业杂志》2009,40(4)
空间位阻、对空气稳定的单氧氯化膦与Pd2(dba)3反应原位形成复合物,可促进芳基硼酸与芳基卤的Suzuki交叉偶联反应。芳基卤上有吸电子基时反应性更高,羰基及氰基无需保护。13例收率66%~93%。 相似文献
52.
目的:研究慢性肝炎患者HBV-DNA水平与病情变化的关系. 方法:采用荧光标记定量PCR的方法,测定慢性乙型肝炎急性发作患者(HBeAg阳性45例,抗HBe阳性30例)其活动期和恢复期的血清HBV-DNA含量. 结果:HBeAg阳性组血清HBV-DNA含量(Logarrithm copy/mL)在活动期显著高于恢复期[(10.2±1.6) vs (5.3±2.1), P<0.05],抗-HBe阳性组血清HBV-DNA含量(Logarrithm copy/mL)在活动期显著高于恢复期[(7.6±1.3) vs (4.0±2.0), P<0.05]. 另外,活动期血清HBV-DNA含量(Logarrithm copy/mL)在HBeAg阳性组显著高于抗HBe阳性组[(10.2±1.6) vs (7.6±1.3), P<0.05]. 血清HBV-DNA水平与丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)及总胆红素(T-BLLI)之间无相关性. 结论:HBV-DNA复制水平与慢性乙型病毒性肝炎的活动密切相关. 相似文献
53.
Serum, plasma and paraffin-embedded tissues as sources of DNA for studying cancer susceptibility genes 总被引:1,自引:1,他引:1
The ability to isolate DNA from archived human serum, plasma and
paraffin-embedded human tissues enhances opportunities to study breast,
lung and other cancer risk factors. We report herein a simple and fast
protocol for the extraction of genomic DNA from these sources. Using a
phenol-based extraction method, the recovery for DNA is quantitative and
reproducible. DNA yields in serum (250 microl) were between 162 and 1060 ng
(n = 18 subjects), in plasma (250 microl) were between 165 and 375 ng (n =
5 subjects) and in embedded tissues (5-microm thick sections for ethanol
fixed, and between 5- and 20-microm sections for formaldehyde fixation)
were between 1 microg and 11.7 microg (n = 32 subjects). The extraction
method was combined with newly designed PCR- based assays for cancer
susceptibility marker genes such as CYP1A1 (exon 7), CYP2E1 (Dra1, Rsa1),
GSTM1 and NAT2 [NAT2*5A (C481T), NAT2*6A (G590A), NAT2*7A (G857A)].
Genotyping results from the serum and paraffin-embedded tissues compared
favorably to results from archived freshly frozen tissues, where
concordance was 98% for serum, 100% for ethanol-fixed embedded tissues, and
97% for formaldehyde-fixed and paraffin-embedded tissues. This facile
method will allow for the use of archived tissue samples of prospective
cohort and other studies where intact DNA was not previously available.
相似文献
54.
Mutation analysis of the Smad2 gene in human colon cancers using genomic DNA and intron primers 总被引:2,自引:0,他引:2
Takenoshita S; Tani M; Mogi A; Nagashima M; Nagamachi Y; Bennett WP; Hagiwara K; Harris CC; Yokota J 《Carcinogenesis》1998,19(5):803-807
In mammals, one of the Mad homologues, Smad2, was reported to be a mediator
of TGF-beta signaling, and was found mutated in some cases of colon and
lung cancers. To extend the analysis of this gene, we previously
investigated the genomic organization of the human Smad2 gene and defined
the structure of 12 exons and flanking introns. In this study, we designed
11 sets of intron-based primers to examine the entire coding region of the
Smad2 gene. By the PCR-SSCP method using these primers, we screened genomic
DNA sequences of colorectal cancers for mutations of the Smad2 gene. Though
there was no mutation within all exons of the Smad2 gene, two of 60
sporadic colorectal cancers displayed deletions in the polypyrimidine tract
preceding exon 4. Deletions of this region were also detected in colon
cancer cell lines, and were clustered within cells exhibiting
microsatellite instability. Deletions in the polypyrimidine tract had
various effects on pre-mRNA splicing, but had no effect on the splicing of
the Smad2 gene in these cases. However, our data support the idea that the
polypyrimidine tract in the splicing acceptor site is a target of mutations
in mismatch repair-deficient tumors.
相似文献
55.
56.
57.
58.
59.
Puijlaert CB; Mali WP; Rosenbusch G; van Straalen AM; Klinge J; Feldberg MA 《Radiology》1986,159(3):635-637
Two cases are reported in which rupture of the renal artery occurred many hours after renal percutaneous transluminal angioplasty. Delayed rupture can be recognized by the angiographic appearance and by the presence of persistent flank pain. The typical angiographic finding is a poorly defined zone of contrast medium at the site of perforation. 相似文献
60.