Electrophysiologic recordings in a patient with a discrete unilateral thalamic infarction

The electroencephalogram, and somatosensory and auditory evoked potentials were recorded from a patient, who, at necropsy, showed a restricted unilateral thalmic infarct involving predominantly the anterior and lateral thalamus. The electroencephalogram showed distinct monomorphic delta activity and a suppression of the alpha rhythm over the side of the lesion. Short latency somatosensory evoked potentials were present bilaterally; mid-latency somatosensory evoked potentials were absent ipsilateral to the lesion. Both mid-latency and long latency auditory evoked potentials were normal.     

果糖氯化钠注射液在急危重病救治中的临床研究

目的观察果糖氯化钠注射液在急危重病救治中对机体内环境的影响。方法将120例急危重病患者随机分为试验组（60例）和对照组（60例）,开放静脉通道的第一瓶液体分别使用果糖氯化钠注射液250ml及0．9％氯化钠注射液250ml,输液速度均为10ml／min。观察两组患者治疗前后的血钾、血钠、血氯、血糖水平变化。结果试验组治疗前后血钾、血钠、血氯、血糖水平变化绝对值分别为（0．26±0．25）mmol/L、（1．7±1．7）mmol／L、（1．5±1．3）mmol／L和（0．7±0．5）mmoL／L,对照组分别为（0．32±0．40）mmol／L、（2．7±3．1）mmol／L、（2．4±2．8）mmol／L和（0．7±0．5）mmol／L,两组患者治疗前后血钠、血氯水平变化绝对值间差异有统计学意义（P〈0．05）,而血钾、血糖水平变化绝对值间差异无统计学意义（P〉0．05）。结论果糖氯化钠注射液可以保持血钾及血糖水平稳定,且血钠、血氯变化值小于0．9％氯化钠注射液,可为急危重患者提供能量而不引起血糖、血钾升高,是一种安全、合适的注射液。     

麦制面粉添加剂对糖尿病的动物实验与临床研究

用含水溶性纤维素、铬和锌的麦制面粉添加剂进行动物实验和临床治疗观察。结果表明，该添加剂可明显地降低血糖、改善血脂异常和糖尿病的一般情况，不引起高胰岛素血症，无明显副作用，容易为患者长期接受。     

Evaluation of the detection of PML-RARα fusion gene in acute promyelocytic leukemia to monitor minimal residual disease

Objective To investigate the kinetics of PML-RARα fusion gene in acute promyelocytic leukemia(APL)to monitor minimal residual disease(MRD). Methods In induction therapy,consolidation and maintenance therapy courses, PML-RARα fusion gene was performed by RT-PCR. Results The long-term follow-up of 18 cases achieved complete remission (CR),two cases experienced molecular relapse. One case relapsed at 4 months after CR1 and achieved CR2 after induction therapy. However, molecular and hematology relapsed again at 2 months after CR2 and re-achieved CR3. The other case relapsed at 74 months after CR1 and achieved CR2 after induction treatment, who had survived for 106 months until the end of follow-up. Conclusion RT-PCR assay for detection of PML-RARα should be performed regularly during CR period so as to find molecular relapse eady. Hematological relapse could potentially be averted through treatment modification according to molecular monitoring results of PML-RARα.     

CXCL12/CXCR4生物轴在头颈部鳞癌淋巴转移的作用

目的 观察趋化因子受体4(CXCR4)及其配体12(CXCL12)在头颈部鳞癌(HNSCC)组织及颈部淋巴结中的表达,分析CXCR4、CXCL12的表达与HNSCC临床病理关系,探讨CXCL12/CXCR4生物轴在HNSCC淋巴转移中的作用.方法 半定量逆转录.聚合酶链反应(RT-PCR法)及免疫组织化学SP法检测65例HNSCC组织、15例正常口腔组织及良性病变中CXCR4、CXCL12基因及蛋白表达.结果 RT-PCR结果:CXCR4在鳞癌转移组、非转移组、良性对照组中的表达分别为0.406±0.044、0.464±0.068、0.900±0.108,各组间表达的差异有统计学意义(P<0.05).转移组、未转移组淋巴结中CXCL12的表达分别为0.935±0.087、0.861±0.047,差异无统计学意义(P>0.05).免疫组织化学结果:CXCR4在鳞癌转移组、非转移组、良性对照组中的表达率分别为71.4%、33.3%、13.3%.各组间表达的差异有统计学意义(X2=65.23,P<0.05).CXCR4的表达与临床分期、肿瘤分化程度、淋巴结转移显著相关(P<0.01),与年龄、性别无明显相关(P>0.05).淋巴结中高表达CXCL12,CXCL12的表达在所有参数的分组表达中差异均无统计学意义(P>0.05).结论 CXCR4的高表达可能赋予HNSCC细胞较强的局部侵犯及淋巴转移潜能;CXCL12/CXCR4生物轴在HNSCC淋巴转移中可能发挥作用.     

伊曲康唑口服液对急性白血病化疗后粒细胞缺乏期侵袭性真菌感染的预防作用

目的评价伊曲康唑VI服液对预防急性白血病化疗后粒细胞缺乏（粒缺）期侵袭性真菌感染（IFI）的疗效。方法回顾性分析2008年1月-2010年12月南方医院血液科收集的急性白血病化疗后伴粒缺的136例患者,将其分为伊曲康唑组（67例）和对照组（69例）。伊曲康唑组中急性非淋巴细胞白血病（ANLL）36例,急性淋巴细胞白血病（ALL）31例;对照组中ANLL30例,ALL38例,1例为急性双表型白血病（BAL）。伊曲康唑组化疗后粒缺期间给予伊曲康唑口服液,持续至中性粒细胞计数〉0．5×10^9／L,或是体温正常且影像学无IFI改变时停用。采用SPSS13．0软件分析两组IFI的发生率及临床特点。计数资料采用PearsonX。检验,正态分布的计量资料采用t检验,偏态分布的数据采用Mann—WhitneyU检验。结果伊曲康唑组12例发生IFI,发生率为17．9％（12／67）,对照组32例发生IFI,发生率为46．4％（32／69）,两组IFI发生率比较差异有统计学意义（X^2=12．59,P〈0．01）。伊曲康唑组中ANLL患者IFI发生率（6／36,16．7％）低于对照组中ANLL患者的发生率（17／30,56．7％）（X^2=11．53,P〈0．01）。伊曲康唑组女性患者IFI发生率（3／35,8．6％）低于男性（9／32,28．1％）（X^2=4．35,P〈0．05）,同时也低于对照组中女性患者IFI发生率（17／38,44．7％）（X^2=11．98,P〈0．01）。结论急性白血病化疗后粒缺期患者预防性口服伊曲康唑可以有效降低IFI的发生,对于急性非淋巴细胞白血病女性患者,疗效更加显著。     

Human erythroid progenitor cells express Rhesus antigens

The expression of Rhesus antigens on hematopoietic progenitor cells was studied using monoclonal antibodies. Because these antibodies are not capable of lysing mature red blood cells in a complement-dependent cytotoxicity assay, fluorescence-activated cell sorting was performed. Using the monoclonal anti-Rh 29 antibody B10, 68% +/- 6% of the mature erythroid progenitor cells (CFU-E) were sorted into the positive fraction, while only 2% +/- 1% of the relatively immature erythroid progenitor cells (BFU-E), and 3% +/- 1% of the granulocyte-macrophage progenitor cells (CFU-GM) were cultured from this same fraction. Thus up to a 15-fold enrichment of CFU-E could be obtained. In two experiments more than 4% of the cells in the positive fraction consisted of CFU-E; in one experiment even more than 7% did. Using fractionated cell sorting, the Rhesus antigens appeared to have a lower density on CFU-E than HLA-DR determinants. Antibodies against the Rhesus antigens can be applied to enrich erythroid-committed stem cells and to separate mature from immature erythroid progenitor cells.