The relation of infectious and hemagglutination titers to the adaptation of influenza virus to mice

The non-pathogenic (unadapted) line of the Rhodes strain of influenza virus multiplied readily in the mouse lung, but its rate of multiplication was slower and its hemagglutination titer attained at the peak of growth was much lower than that of the adapted line of the same virus. The implication of these findings to mouse adaptability of the virus is discussed.     

Ilizarov外固定器治疗马蹄内翻足的护理

[目的]总结13例马蹄内翻足应用外固定器矫形的护理。[方法]采取心理疏导、针孔护理、密切观察肢体感觉、运动功能,指导正确使用外固定器及康复训练等综合护理。[结果]随访13例患者术后疗效满意,无1例因护理不当出现并发症。[结论]应用外固定器治疗,通过围手术期精心护理,保证了手术的成功,达到了病人的满意。     

子宫内膜体外构建及其应用研究进展

体外构建子宫内膜是利用组织工程学原理,在体外构建结构、形态与功能上与体内子宫内膜相似的三维模拟体。种子细胞分离培养、生物支架材料的合理选择及体外构建方法的优化是体外构建子宫内膜的重要因素。构建的子宫内膜在病理研究、三维培养体系、胚胎发育学及移植等方面具有重要的研究意义。只有更进一步模拟自然子宫的结构,优化构建技术路线和培养条件,才可以再造出真正意义上的组织工程化子宫。     

Anti-inflammatory effect of erythropoietin pretreatment on cardiomyocytes with hypoxia/reoxygenation injury and the possible mechanism

Objective： To investigate the anti-inflammatory effect of erythropoietin （EPO） pretreatment on cardiomyocytes exposed to hypoxialreoxygenation injury （H/R） and explore the possible mechanism.
Methods： The cultured neonatal rats＇ ventricular cardiomyocytes were divided randomly into 4 groups, control group （C group）, EPO pretreatment group （E group）, EPO and pyrrolidine dithiocarbamate （PDTC） pretreatment group （EP group） and PDTC pretreatment group （P group）. After 24 hours＇ pretreatment, the cardiomyocytes were exposed to H/R. After pretreatment and H/R, the expression of tumor necrosis factor- α （TNF- α ） gene in all the groups was detected by RT-PCR and Western blot. The nuclear factor- κ B （NF- κB） activity was detected by electrophoretic mobility shift assay （EMSA） and the inhibitor- κB α （Ⅰ- κB α） protein level was detected by Western blot.
Results： The decrement of Ⅰ- κB a protein and the increasing NF- KB activity were found in cardiomyocytes pretreated with EPO before H/R compared to other groups （t=3.321, 4.183, P〈0.01）. However, after H/R, NF- κB activity and expression of TNF- α gene were significantly reduced, Ⅰ- κB a protein expression was increased in cardiomyocytes of E group compared to other groups （t=-3.425, 3.687, 3.454, P〈0.01）. All theses changes caused by EPO pretreatment were eliminated by the intervention of PDTC （an antagonist to NF- κB） during pretreatment.
Conclusions： EPO pretreatment can inhibit the activation of NF- κB and upregulation of TNF- α gene in cardiomyocytes exposed to H/R through a negative feedback of NF- κB signaling pathway, and thus produces the anti-inflammatory effect. This might be one of the ways EPO produces the anti-inflammatory effect.     

妇产科理论授课方法改革的尝试

改革妇产科理论课讲授。改革课程设置，以病例为中心讲授，把讨论教学形式引入课堂，取得较好效果。     

微创穿刺术治疗基底节区脑出血临床随机对照研究

目的评价比较微创穿刺血肿粉碎清除术与内科保守治疗两种方法治疗基底节区脑出血(25～40m l)的疗效异同。方法采用多中心、随机对照试验的方法,42个参研医院共随机入选465例基底节区脑出血患者,根据纳入与排除标准共排除88例,其中资料不全者16例;不符合入选标准者72例,分别为Glasgow评分≤8分(64例)、术前出血量>40m l(7例)、从发病到达急诊室时间>72h(1例)。评价治疗14d时两组患者神经功能缺损程度和日常生活活动能力、治疗3个月时的日常生活活动能力以及3个月和住院期间病死率。结果最终符合入组标准的病例数为377例,其中微创治疗组195例,对照组182例。微创治疗组患者于治疗14d时,神经功能改善明显优于对照组(χ2=7.931,P=0.02);治疗3个月时达良好功能状态的患者比例明显多于对照组(35.91%vs21.82%;χ2=8.294P=0.004)。微创治疗组病,残率明显低于对照组(40.88%vs63.03%,χ2=16.948,P<0.01);两组病死率间差异无显著性意义(6.67%vs8.79%)。结论与单纯内科保守治疗相比,应用微创穿刺血肿粉碎清除术治疗基底节区小血肿不增加病死率,并可明显提高脑出血患者的日常生活活动能力,降低病残率。     

脑弥漫性轴索损伤40例临床诊治

脑弥漫性轴索损伤(diffuse.axonal.injurg,DAI)是由Adrims等在1982年正式命名.并被国际学术界所公认.……     

四腔双囊管治疗老年尿闭临床观察（附5例报告）

目的:观察四腔双囊管局部治疗老年尿闭的疗效。方法:2%普鲁卡因溶液10ml作尿道麻醉,将消毒后的四腔双囊管经尿道插入膀胱,并留置3天,每天冲洗膀胱及尿道2次,并经注药腔注药于前列腺,并维持压力恒定。每次治疗40～50分钟。结果:治愈4例,好转1例。有效率:100%,治愈率80%。结论:采用四腔双囊管治疗老年尿闭,损伤小,疗效显著。