Antiarrhythmic activity of GABA derivative TZ-50-2

The GABA derivative TZ-50-2 exerted pronounced antiarrhythmic effects on a variety of arrhythmias (atrial, ventricular, and mixed). The drug was superior (or at least comparable) to quinidine, procainamide, lidocaine, verapamil, bonnecor, and other reference drugs in antiarrhythmic activity and therapeutic range, and showed no cardiotoxicity. The antiarrhythmic effects of TZ-50-2 were due to modulation of calcium and sodium channels. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 4, pp. 415–418, April, 1999     

Establishing and managing a periodontal biobank for research: the sharing of experience

Periodontal bio‐repositories, which allow banking of clinically validated human data and biological samples, provide an opportunity to derive biomarkers for periodontal diagnosis, prognosis and therapeutic activities which are expected to improve patient management. This article presents the establishing of the Malaysian Periodontal Database and Biobank System (MPDBS) which was initiated in 2011 with the aim to facilitate periodontal research. Partnerships were established with collaborating centres. Policies on specimen access, authorship and acknowledgement policies were agreed upon by all participating centres before the initiation of the periodontal biobank. Ethical approval for the collection of samples and data were obtained from institutional ethics review boards. A broad‐based approach for informed consent was used, which covered areas related to quality of life impacts, genetics and molecular aspects of periodontal disease. Sample collection and processing was performed using a standardized protocol. Biobanking resources such as equipment and freezers were shared with the Malaysian Oral Cancer Database and Tissue Bank System (MOCDTBS). In the development of the MPDBS, challenges that were previously faced by the MOCDTBS were considered. Future challenges in terms of ethical and legal issues will be faced when international collaborations necessitate the transportation of specimens across borders.     

新缩瞳剂包公藤甲素人工合成研究

包公藤甲素是从包公藤(Erycibe obtusifolia Benth.)茎中提得的一个新莨菪烷生物碱,具有强烈的缩瞳作用,临床用于治疗青光眼。本文报道用合成的6β-乙酰氧基托品酮为原料,经卤代、水解、还原和N-去甲基化等反应合成包甲素(8)。经光谱测定证实8与天然包甲素的结构完全一致。合成品系外消旋体,其作用机理与天然品相同,而强度则减半。     

肿节风片的质量标准研究

目的：制订肿节风片质量标准。方法：紫外分光光度法测定了总黄酮的含量,对肿风药材进行了薄层色谱鉴别。结果：加标回收率平均９８．１％（ＲＳＤ＝２．３％,ｎ＝６）,ｒ＝０．９９９９６,重复性ＲＳＤ＝１．２３％（ｎ＝６）,精密度ＲＳＤ＝１．１４％,结论：方法稳定,可靠,可作为该制剂的质量控制方法之一。     

Clinical Analysis of 57 Patients with Ovarian Dysgerminoma

Objective  Ovarian dysgerminoma is an uncommon ovarian malignancy, Its clinicai features are special and there are many factors affecting its prognosis. If treated properly, the patient can be cured. Otherwise it may endanger the patient’s life. The aim of this study is to investigate the clinical features and factors related to prognosis of ovarian dysgerminoma. Methods  Data from 57 patients with pure ovarian dysgerminoma were analyzed retrospectively. The patients were admitted to the Cancer Center, Sun Yat-sen University from January 1.1964 to December 31, 2000. Results  The main clinical features were abdominal mass (56.1% ), abdominal pain (21.1% ), abdominal swelling (17.5%.), vaginal bleeding (5.3% )and genital tract abnormalities (5.3%). Twenty-six patients had stage I diseases, 8 stage II.9 stage III.1 stage IV and 13 recurrent and persistent diseases. The uterus was involved in 41.2% of patients with stage II -III diseases. Combined modality was given to 52 cases and a single-method treatment to 5 cases. The total overall 5 and 10-year survival rates for stages I-IV was 80.1 % and 70.0% respectively. The 5-year survival rate for stage I was 100%, stage II 55.2%. stage III 55.6% and stage IV 0%; for recurrent and persistent diseases, 72.7%. The stage I group of 12 patients. received adnexectomy and 14 patients underwent hysterectomy and adnexa removal. There was no significant difference between the 5 and 10-year survival rates (all 100%). Of the 23 patients in the stage I group to whom oniy chemotherapy was given after operation, 19 cases received 3 or more courses and were well without recurrence; 4 patients received only one course and one of them recurred 21 months after the operation. In the group of stages II and III cases, the 5-year survival rate was 86.7% for those whose chemotherapy courses were 3≥ 4 and 25.0% for patients who received less than 4 courses of chemotherapy (P<0.05). Conclusions  The prognosis of ovarian dysgerminoma is closely related to the disease stage and treatment modality. A fertility-preserving operation can be considered in early -staged patients, but caution needs to be exercised in the middle to late staged cases. Good results can be achieved with an operation-based combined modality in recurrent patients.     

Effect of adrenaline on the quality of postoperative epidural analgesia

The aim of the study was to assess the efficacy and safety of patent controlled analgesia (PCEA) combining opioid-local anesthetic mixture and adrenaline administered after thoracotomy and laparotomy. 164 adult patients after major thoracic and abdominal surgery were enrolled in prospective randomized study. All patients were given PCEA using 0.125% bupivacaine and fentanyl (2 mcg/ml) eithr without (BF group, n = 73) or with adrenaline (2 mcg/ml) (ABF group, n = 91). Pain scores were assessed in rest and coughing by 100-point visual analog scale (VAS) at 3, 6, 12 and 24 hours after ICU admission. In addition, the consumption of medication and the incidence of adverse effects (pruritis, urine retention, and nausea/vomiting) were recorded. Data were compared by the means of Student's t-test and chi2 test. p < 0.05 was regarded as statistically significant. VAS in coughing was significantly lower in the ABF group at 3rd 6th 12th, 18th and 24th hour after ICU admission. The consumption of medication requested for the adequate analgesia (VAS < 3) decreased in the ABF group compare to the BF group. The number of incidence of side effects was lower in the ABF group. The outcome of the study shows that use of PCEA with bupivacaine--fentanyl mixture with adrenaline after major surgery reduces the consumption of both bupivacaine and fentanyl and lowers the incidence of adverse effects, therefore improving the quality of analgesia.     

Regulation of Toll-like receptor 1 and -2 in neonatal mice brains after hypoxia-ischemia

Background  

Hypoxic-ischemic (HI) brain injury remains a major problem in newborns, resulting in increased risk of neurological disorders. Neonatal HI triggers a broad inflammatory reaction in the brain, including activation of the innate immune system. Toll-like receptors (TLRs), which are key components of the innate immune system, are believed to play a role in adult cerebral ischemic injury. The expression of TLRs in the neonatal brain and their regulation after HI is unknown.