辛伐他汀对大鼠胫骨骨折愈合的促进作用及其相关信号通路研究

目的 探讨辛伐他汀对大鼠胫骨骨折愈合的促进作用及其相关信号通路。方法 建立64只SD大鼠右侧胫骨骨折模型,随机分为短期用药组、渐撤量给药组、连续用药组和对照组。术后第1,2,3,4周,每组随机选择4只大鼠处死,分别从X线检查、骨组织HE染色、血清RANKL、OPG水平分析差异。结果 X线检查：术后4周,渐撤量给药组与连续用药组骨折线愈合良好,仅有少量骨痂残留;短期用药组仍可见明显骨痂及骨折线。骨组织HE染色：术后2周,用药组可见编制样骨开始形成,并可见造血细胞灶;术后4周,短期用药组有板层样骨形成,但骨排列方向紊乱,渐撤量给药组可见骨基质中大量骨细胞沉积。血清RANKL水平：术后2周,短期用药组血清RANKL水平明显低于对照组（P<0.05）;连续用药组血清RANKL水平随时间逐渐下降的趋势最明显;术后第3周,短期用药组血清RANKL水平达到峰值,显著高于其他3组。血清OPG水平：术后第2周,各用药组血清OPG水平显著高于对照组（P<0.05）,术后第4周,短期用药组血清OPG水平最高（P<0.05）。结论 辛伐他汀对骨折愈合有一定的促进作用,但骤然停药对骨折愈合会造成明显的不良影响。     

慢性前列腺炎中医证型与血清前列腺特异性抗原的相关性

目的：通过检测慢性前列腺炎患者的血清前列腺特异性抗原（PSA）值,探讨慢性前列腺炎证型与血清PSA的关系.方法：选取门诊符合NIH慢性前列腺炎诊断标准患者149例,严格按中医辨证分为湿热下注,脾肾气虚,气滞血瘀,肾阴亏虚四组;用化学发光微粒子免疫法检测各组血清PSA水平.结果：湿热下注与气滞血瘀两组PSA水平有显著性差异（P＜0.01）,脾肾气虚,肾阴亏虚二个证型与PSA水平无显著性差异（（P＞0.05）.结论：湿热下注证与气滞血瘀证对PSA变化影响较大,湿热下注证与气滞血瘀证均与PSA水平呈正相关,而脾肾气虚证、肾阴亏虚证与PSA水平相关性不大.     

Estimation of stored energy in deformed metals from EBSD data sets

A number of methods for estimating the stored energy of deformation from EBSD data have been examined. The methods are illustrated here using samples of AA1100 deformed to strains ofε oM=2.4 and εoM=4.0 using the accumulative roll bonding process. A qualitative method, based on pattern contrast, reveals some aspects of local differences in stored energy in the deformed samples. Quantitative methods where the stored energy is estimated through the use of the Read-Shockley equation have also been exam- ined. A. special mean misorientation angle is developed to take account of nonlinearity of the Read-Shock-ley equation. Two approaches have been considered. One is based on subgrain reconstruction and the other is based on misorientation angles taken directly from an orientation map. Calculations show that the subgrain method is particularly sensitive to orientation noise in the data and care must be taken when using this method.     

Genetic association of PLCE1, C11orf92-C11orf93, and NOC3L with colorectal cancer risk in the Han population

Colorectal cancer (CRC) is a common malignant tumor that is influenced by an interaction between genetic and environmental factors. Currently, the inherited factors of CRC are unclear. Our study selected 19 tag single nucleotide polymorphisms (tSNPs) to investigate whether they were associated with CRC in the Han population. In this Han Chinese case–control study, we genotyped 203 CRC cases and 296 controls using Sequenom MassARRAY technology and analyzed their associations with CRC using χ² tests, SNPStats software, and SHEsis software. Based on χ² tests, PLCE1 -rs2077218, rs11187877 (p?=?0.049) and C11orf92-C11orf93-rs3802842 (p?=?0.023) correlate with CRC risk. In the genetic model analyses, we found the genotype “CC” of rs3802842 in C11orf92-C11orf93 may significantly increase CRC risk in the recessive model (p?=?0.0071), whereas “GT” of rs17109928 in NOC3L may decrease the risk in the over-dominant model (p?=?0.0091). Using SHEsis software, we found PLCE1 and NOC3L are strongly linked, and the “GCCATTCTGTC” haplotype may increase the risk of CRC (p?=?0.049). We found three genes (PLCE1, C11orf92-C11orf93, and NOC3L) are associated with CRC susceptibility. In combination with previous reports, our results suggest that these genes may be associated with CRC in the Han population.     

小儿外伤性脑梗塞25例临床分析

目的：探讨小儿外伤性脑塞的临床特点,发病机理,诊疗及预后,方法：全部患儿予行头颅ＣＴ平扫,予扩血管、解痉、神经营养及抗凝治疗。１例合并阻塞性脑积水行脑室腹腔分流术,１例合并左颞硬膜下血肿予开颅血肿清除术。结果：全血患儿均治愈出院,预后良好。结论：ＣＴ扫描和追踪观察对本病诊断的确有重要价值。外伤后脑的微循环障碍和脑血管痉挛为本病形成的主要原因,及早发现和治疗预后良好,对合并其他颅内损伤应早期诊治。     

腺苷酸激活蛋白激酶激活参与促进慢性缺氧时心肌细胞存活

目的 检测腺苷酸激活蛋白激酶(adenosine monophosphate-activated protein kinase,AMPK)在慢性缺氧心肌组织中的激活水平,探讨AMPK激活对慢性缺氧心肌细胞存活的影响及其意义.方法 选择本科2013年行手术矫治的先心病患儿24例,其中紫绀型先心病患儿12例,非紫绀型先心病患儿12例,取手术中切除的右室流出道心肌组织作为标本;选用4～6周龄清洁级雄性SD大鼠24只,分为常氧组和缺氧组(n=12).将缺氧组置入低压舱(模拟海拔5000m,气压405 mmHg),常氧组正常饲养.同时喂养28 d后,处死大鼠后迅速取出心脏并分离出右心室部分作为标本.采用Western blot分别检测p-AMPK在人体、大鼠缺氧心肌组织中的激活水平.选用H9c2心肌细胞株,并将其分为常氧组、低氧组、阻断组、激活组.将后3组细胞置入缺氧培养箱3 d (94％ N2,5％ CO2,1％ O2),建立H9c2心肌细胞株慢性缺氧模型;常氧组置入细胞培养箱3d,对照培养.分别向阻断组和激活组中加入AMPK特异性阻断剂Compound C和激活剂AICAR,采用Western blot检测p-AMPK的表达,流式细胞仪、TUNEL法检测心肌细胞凋亡,Hoechst33528染色观察细胞核形态、LDH测定心肌细胞死亡率.结果 Western blot结果显示与常氧组相比,缺氧心肌组织中p-AMPK/AMPK比值明显增高.与低氧组比较,AMPK阻断组凋亡细胞比例升高,TUNEL-阳性细胞比例、不正常核比例、细胞死亡率均明显升高(P ＜0.05,P＜0.01);AMPK激活组与低氧组比较,凋亡细胞比例、TUNEL-阳性细胞比例、不正常核比例、细胞死亡率均有降低(P＜0.05).结论 在慢性缺氧条件下,AMPK蛋白磷酸化水平增强,p-AMPK对心肌细胞慢性缺氧适应具有一定保护作用.     

结直肠癌患者血清游离DNA的表达及意义

目的:建立基于ALU序列的实时荧光定量PCR(ALU-q PCR)检测游离DNA浓度的方法,并探讨结直肠癌患者血清游离DNA定量检测的临床意义。方法:随机采集结直肠癌104例、结直肠息肉63例和正常人110例血清标本,采用ALU-q PCR方法定量检测血清ALU115表达水平;用化学发光方法检测肿瘤标志物CEA的水平。结果:(1)结直肠癌患者血清ALU115浓度中位数为1046.0 ng/m L,显著高于结直肠息肉组423.3 ng/m L和对照组385.4 ng/m L,差异有统计学意义(P均<0.001),结直肠息肉组和对照组比较差异无统计学意义(P>0.05)。(2)结直肠癌血清ALU115和CEA联合检测灵敏度、特异性、准确度、阳性预测值和阴性预测值分别是82.69%、99.09%、91.12%、98.85%和85.82%,两者联合检测提高结直肠癌诊断率。结论:基于ALU序列的实时荧光定量PCR是一种快速简便、灵敏度高及特异性好的检测方法。血清cf-DNA浓度对结直肠癌辅助诊断有一定的价值。