Nanoscale Au@SiO2-drug/VEGF as an in vivo probe for osteosarcoma diagnosis and therapy

Osteosarcoma is a common primary bone malignancy, with a 5-year survival rate of only 20–30% in patients undergoing surgical treatment. Thus, it is important to identify novel methods for diagnosing and treating osteosarcoma, which was the aim of the present study. Vascular endothelial growth factor (VEGF) was used as the tumor-targeting protein to synthesize a multifunctional core-shell nanostructure, Au@SiO₂-drug/VEGF, in which the drug can be indocyanine green (ICG; as an optical tracer) or doxorubicin (DOX; as a chemotherapeutic agent). With VEGF as the osteosarcoma-targeting protein, Au exhibited optimal photothermal transformation performance, while SiO₂ served as the carrier for the drug. Au@SiO₂-ICG/VEGF nanoparticles (NPs) were evaluated for imaging and for the monitoring of drug accumulation in a tumor region in mice. Once the optimal drug accumulation was achieved, combined treatment of osteosarcoma (chemotherapy and photothermal therapy) was assessed. In the perioperative period associated with minimal invasive embolization of osteosarcoma, photothermal therapy and chemotherapy were applied for osteosarcoma diagnosis using Au@SiO₂-DOX/VEGF NPs. Taken together, the results of the present study provide a promising strategy for tumor detection prior to surgical treatment to improve the survival outcome of patients with osteosarcoma.     

LMR与妇科恶性肿瘤预后的研究进展

大量医学研究已证实炎症与肿瘤的发生、发展密切相关。在近年来的医学临床研究中,也逐渐证实并阐述了肿瘤相关性炎症反应在肿瘤的预后中发挥着重要作用。而且,肿瘤相关性炎症反应可通过患者的中性粒细胞、淋巴细胞和单核细胞等多种外周血象指标反映出来,同时,肿瘤患者的外周血淋巴细胞与单核细胞的比值,即淋巴细胞/单核细胞(LMR),在妇科恶性肿瘤的诊断及预后中发挥着重要作用。在国内外很多研究中,已将LMR在消化、血液等多个系统发生的恶性肿瘤预后中的重要作用给予阐述,而其在妇科生殖系统的肿瘤领域的研究进展,国内外鲜有报道。因此,本文主要就LMR对妇科常见恶性肿瘤预后的研究进展进行综述。     

营养不良风险的慢性乙型肝炎患者甲胎蛋白、高尔基体糖蛋白73表达分析

目的：研究应用营养筛查评分简表（NRS2002）评分在甲胎蛋白（AFP）、高尔基体糖蛋白73（GP73）高表达的患者中的应用价值。方法：对206例肝炎患者体重指数（BMI）、白蛋白、AFP、GP73的测定,利用NRS2002评分简表对患者进行评分,将患者分成营养风险组（A组）和非营养风险组（B组）,采用统计学方法对A、B组两组患者的AFP、GP73表达进行分析。结果：营养风险组所检测的AFP、GP73高表达率显著高于非营养风险组（P〈0.05）;营养风险组的CHB、肝硬化、原发性肝癌（PHC）患者AFP、GP73的表达逐渐增加;NRS2002分值越高,AFP、GP73表达亦增高。结论：结合NRS2002评分能更多地将无AFP、GP73高表达的患者被正确区分,从而提高AFP、GP73高表达的检出率。     

芫花条化学成分的研究

从芫花Ｄａｐｈｎｅ ｇｅｎｋｗａ条的乙酸乙酯可溶部分分到３个结果性化合物，借助于波谱分析手段推断出Ⅱ为西瑞香素，Ⅲ为ｄａｐｈｎｏｄｏｒｉｎ Ｂ。     

雅博司治疗乙肝肝硬化引起的肝性脑病26例疗效观察

目的 观察雅博司治疗乙肝肝硬化引起的肝性脑病的临床疗效。方法 将48例乙肝肝硬化出现肝性脑病患者随机分为雅博司组（26例）和乙酰谷酰胺组（22例）,治疗1周后观察疗效。结果 雅博司组显效12例（46．2％）,有效13例（50％）,无效1例（3.8％）,无死亡,总有效率为96．2％;而乙酰谷酰胺组显效5例（22．7％）。有效7例（31．8％）,无效6例（27．3％）,死亡4例（18．2％）,总有效率为54．5％。两组总有效率比较差异有显著性（P〈0．01）。雅博司组血氨浓度显著降低（P〈0．05）。结论 雅博司是治疗乙肝肝硬化患者肝性脑病的有效药物,可迅速控制惠者的临床症状,降低死亡率。     

多重耐药鲍曼不动杆菌血流感染的危险因素和预后分析

目的探讨多重耐药(MDR)鲍曼不动杆菌血流感染的危险因素及其对患者预后的影响。方法采用病例对照的研究方法,回顾性收集昆明医科大学第一附属医院2013-2018年鲍曼不动杆菌血流感染患者142例(142株),以MDR鲍曼不动杆菌血流感染111例(111株)为观察组,非多重耐药(NMDR)鲍曼不动杆菌血流感染31例(31株)为对照组,进行血流感染的危险因素分析;按预后分为预后不良组81例和好转组61例,进行预后的危险因素分析。结果鲍曼不动杆菌对常用抗菌药物耐药率高,除替加环素外,MDR鲍曼不动杆菌组的耐药率均高于NMDR鲍曼不动杆菌组,差异均有统计学意义(P<0.05)。MDR鲍曼不动杆菌组病死率高于NMDR鲍曼不动杆菌组(43.2%比3.2%),差异有统计学意义(P<0.05);MDR鲍曼不动杆菌组预后不良发生率高于NMDR组(69.4%比12.9%),差异有统计学意义(P<0.05);多因素分析结果显示呼吸衰竭、初始抗感染治疗不当是MDR鲍曼不动杆菌血流感染的独立危险因素。呼吸衰竭、使用导尿管、初始抗感染治疗不当、肺部感染是影响鲍曼不动杆菌血流感染预后的独立危险因素。结论MDR鲍曼不动杆菌血流感染所致的病死率较NMDR鲍曼不动杆菌病死率高。呼吸衰竭、初始抗感染治疗不当是MDR鲍曼不动杆菌血流感染的独立危险因素。呼吸衰竭、使用导尿管、初始抗感染治疗不当、肺部感染是影响鲍曼不动杆菌血流感染预后的独立危险因素。     

Lung fluid absorption is induced in preterm guinea pigs ventilated with low tidal volumes

The objective of this study was to determine if low tidal volume (V(t)) ventilation was beneficial when ventilating preterm fetuses. The authors ventilated preterm guinea pig fetuses at gestation day (GD) 67, 3 days before birth, newborn, and 10-day-old (PD10) guinea pigs with low V(t) (6 mL/kg body weight [bw]) and compared them to age-matched fetuses/animals ventilated with higher potentially injurious V(t) (12 mL/kg bw). Lung fluid absorption was measured after intratracheal instillation of 5% albumin in 0.9% NaCl. Low V(t) ventilation stimulated lung fluid absorption when compared to higher V(t) in all groups. The increased lung fluid absorption in low V(t)-ventilated fetuses was associated with increased α epithelial Na channel (αEnaC) mRNA. However, αENaC and βENaC protein was unchanged over the 1-hour study. Because stretch induces mitogen-activated protein (MAP) kinase expression and MAP kinases may affect lung fluid absorption, the authors investigated if MAP kinase (MAPK) expression was affected by V(t). Extracellular signal-regulated kinase (ERK) and MAPK/ERK kinase (MEK) were phosphorylated in the higher V(t)-ventilated guinea pig fetuses. This suggested that a reduced activation of MAP kinases might explain the increased lung fluid absorption in the low V(t)-ventilated fetuses. Thus these data suggest that low V(t) ventilation increases fetal lung fluid absorption and thus may be preferential to use clinically.     

江苏中燃油品储运有限公司医学媒介生物本底调查报告

目的摸清江苏中燃油品储运有限公司媒介生物的本底情况,为开展媒介生物控制工作提供科学依据。方法按照出入境检验检疫行业标准SN／T1240-2003、SN／T1300—2003、SN／T1313—2003、SN／T1191—2003、SN／T1292—2003、SN／T1293—2003、SN／T1294—2003、SN／T1339—2003进行。结果全年共布鼠夹4536只,有效夹4418只,共捕获鼠28只,隶属1科2属3种,黄胸鼠为该公司所在地的优势鼠种,鼠类年平均密度为0．64％,6～9月份相对鼠密度较高;全年共放置诱蚊器90次,捕获蚊虫981只,隶属1科2亚科2属2种,淡色库蚊是该公司所在地的主要优势种,蚊类年平均密度为10．9只／笼,6～10月份为蚊类密度的高峰期;全年共放置诱蝇笼120只,捕获蝇类25490只,隶属4科13属17种,大头金蝇是该公司所在地的主要优势蝇种,蝇类年平均密度为213．4只／笼,6～10月份是蝇类活动的高峰期;全年共放置诱捕盒1080个,捕获蜚蠊23只,隶属2科2属3种,德国小蠊是该公司所在地的主要优势种,年平均蜚蠊密度指数为0．021只／瓶,7、8月份密度最高;在鼠夹捕获的28只鼠体上,未能发现染蚤鼠;全年共拉蜱108人次,经检查,未发现蜱类;全年共放置诱捕灯108次,经检查,未能捕获蠓类;结合鼠类调查,在鼠夹捕获的28只鼠体上,未能采集到螨类。结论在媒介生物防治工作中应采取综合性防治措施,加强监测,作好环境治理,确保媒介生物密度符合国家标准。