NT—3、NT—4和BDNF在面神经逆行转运的动力学比较

OBJECTIVE: To compare the kinetic characters of retrograde transport of neurotrophin-3 (NT-3), neurotrophin-4 (NT-4) and brain-derived neurotrophin factor (BDNF) in facial nerve. METHODS: Radioactive tracer technique was used. After one lateral facial nerve trunk of adult rabbit was transected, a silicone chamber was inserted between the stumps, and 3.7 MBq of 125I-NT-3 or 125I-NT-4 or 125I-BDNF or 125I-HSA was administered into the chamber. At different time-points after injection, the facial nerve trunk and facial nerve motor neurone of brain-stem were collected and the uptake rates were measured. The kinetic parameters of each labeled compound were calculated using 3P87 program of kinetics. RESULTS: The transport amount of neurotrophin retrogradely transported by facial nerve is NT-3 > BDNF > NT-4 (P < 0.05), the transport rate is NT-4 > NT-3 > BDNF (P < 0.05). CONCLUSION: The findings could serve as the kinetic characters of retrograde transport of neurotrophins.     

新型基因重组藻胆蛋白对小鼠S180实体瘤COX-2表达的影响

目的探讨新型基因重组藻胆蛋白对小鼠S180实体瘤环氧化酶-2(COX-2)表达的影响。方法将小鼠腋下接种S180细胞荷瘤造模后,随机分为阴性组、环磷酰胺组、新型基因重组藻胆蛋白组[50mg/(kg·d)]、藻胆蛋白低、高浓度组[25mg(kg·d),100mg/(kg·d)],共5组,阴性组给予等量生理盐水,灌胃给药11天后处死,剥离肿瘤,采用免疫组化方法检测肿瘤组织中COX-2的表达。结果新型基因重组藻胆蛋白组、藻胆蛋白低、高浓度组突变型COX-2平均阳性表达率分别为28.64%、42.38%、31.68%,均低于阴性组66.12%(P<0.05)。结论新型基因重组藻胆蛋白可明显抑制COX-2表达,可能对COX-2表达有下调作用。     

COX-2在系统性红斑狼疮病人PBMC中的表达及意义

目的：检测环氧全酶-2(Cyclooxygenase-2，COX-2)蛋白在系统性红斑狼疮(Systemic lupus erythematosus．SLE)患外周血单核细胞(Peripheral blood monoeytes，PBMC)中的表达情况。探讨COX-2蛋白表达水平与SLE病情活动程度之间的关系。方法：从外周血中分离PBMC后涂片，用免疫组化染色的方法测定COX-2蛋白的表达情况。结果：COX-2蛋白在SLE实验组呈高表达状态，与SLE疾病的活动性之间呈正相关。结论：COX-2的表达与SLE的发病有关，可作为今后辅助治疗措施的目标和病情变化和疗效评价的参考指标之-。     

Successful removal of a potentially lethal left atrial thrombus detected by transesophageal echocardiography following the removal of a left ventricular assist device inflow cannula

The left ventricular assist device (LVAD) is a battery-operated, mechanical pump-type device that helps in maintaining the pumping ability of a failing heart. Thromboembolism poses a significant risk during and after LVAD implantation. It occurs in up to 35% of patients with adverse sequelae. We present the case of a 75-year-old man who underwent coronary artery bypass graft surgery and LVAD implantation for acute myocardial ischemia and severe left ventricular dysfunction. However, subsequent transthoracic echocardiographic examination revealed an LVAD thrombus, and LVAD removal was suggested following the failure of thrombolytic therapy. After the LVAD cannula was removed, transesophageal echocardiography (TEE) revealed a residual thrombus in the left atrium. Thrombectomy was successfully performed by opening the left atrium with cardiopulmonary bypass. We believe that TEE monitoring aided the implantation and removal of the LVAD device. In this case, we found that TEE not only helped in monitoring the ventricular function but also in detecting other problems such as the residual thrombus. We strongly recommend TEE monitoring during the entire LVAD-removal procedure, particularly for patients who need to undergo LVAD removal because of thrombosis formation.     

冠心病合并甲状腺功能亢进33例临床分析

目的探讨冠心病合并甲亢的临床特征,提高临床诊疗水平。方法回顾性分析我院1993年-2008年的33例冠心病合并甲亢患者的临床资料,根据临床症状体征、心脏彩超、心电图、运动平板试验、核素和冠状动脉造影等结果进行分析比较。结果33例冠心病合并甲亢患者中有冠心病史15例,甲亢病史10例,糖尿病24例,高血压18例,吸烟12例;均表现胸闷、心悸,合并胸痛21例,心律失常30例,心功能不全22例,体重减轻21例,怕热、皮肤湿润15例,暴躁、失眠8例,大便次数增多12例,甲状腺肿大6例。入院时均未考虑冠心病并甲亢,明确诊断后给予抗冠心病和抗甲状腺药物治疗。好转出院26例,病重自动出院2例,死亡5例。结论临床应重视冠心病合并甲亢的诊断：对冠心病反复出现顽固性心力衰竭、心绞痛,常规抗冠心病抗心力衰竭治疗效果欠佳时,注意存在不明原因消瘦、多汗、便次增多、失眠、暴躁、手颤等症状,应及时做甲状腺功能测定;对初诊甲亢患者,若伴有冠心病危险因素,出现心力衰竭、心绞痛、心律失常等表现应及时做心电图、心脏B超、运动平板试验及核素等检查,必要时做选择性冠状动脉造影。明确诊断为冠心病合并甲亢应及时给予抗冠心病和抗甲状腺等药物联合治疗。     

抑郁症常见中医证候临床流行病学调查的Kappa一致性检验

目的 :检验抑郁症常见中医证候临床流行病学调查表的可靠性。方法 :随机选择 30例抑郁症患者 ,分别由两名专家对同一病例 ,同时进行调查 ,然后计算其中主要指标的一致系数 (Kappa ,κ值 )。结果 :各组专家对抑郁症的西医诊断的一致性最高 (κ=1.0 ) ;中医辨证、中医证候指标总积分及情志抑郁、烦躁等 5项主要证候指标具有高度的一致性 (0 .6 <κ <0 .8) ;而少寐、纳差及汉密尔顿 (HAMD)抑郁量表评定总分亦具有中度的—致性 (0 .4 <κA <0 .6 )。结论 :抑郁症常见中医证候临床流行病学调查表具有较好的可靠性和实用性。     

心理干预在肾移植手术中的应用

目的分析心理干预对肾移植患者手术前心理状况的影响。方法30例择期行肾移植手术的患者随机分为两组:心理干预组和对照组,每组15例,采用ZUNG抑郁自评量表(SDS)、ZUNG焦虑自评量表(SAS)对心理干预组和对照组进行比较,针对患者症状严重程度分别进行心理干预前、后评分,同时记录不同时段HR、RPP。结果肾移植患者手术前存在异常心理,心理干预组焦虑、抑郁总分明显下降,与治疗前相比较差异性有显著性意义(P<0.05)。结论肾移植患者术前进行心理干预,能够提高患者的治疗信心,改善异常心理和提高生活质量。     

医学检验技术专业实验教学管理体系的探讨

本文结合怀化医学高等专科学校医学检验技术专业的实际情况,从实验教学计划的制定、教学大纲的修订、教学内容的调整、综合设计性实验的开展和实验考核的实施等方面,进行了实验教学管理体系改革的研究与实践,取得了良好的效果.     

甲氨蝶呤对映体诱导肺癌细胞耐药后引起血管内皮细胞分化差异的研究

目的 研究甲氨蝶呤(MTX)对映体大剂量冲击作用肺癌A549细胞后,MTX对映体耐药细胞血管分化是否具有手性选择性.方法 用大剂量冲击法诱导获得A549对映体耐药肺癌细胞,流式细胞双色分析细胞CD44/CD31以及P-170表达;皮下接种裸鼠,成瘤后取瘤体切片,进行CD34组化染色,计数新生微血管密度(MVD).以A549亲本细胞(未获得耐药)作为对照组.结果 分别建立耐药浓度为15 μmol/L的L-MTX和D-MTX的A549耐药细胞株;它们与各自对映体的耐药指数分别为12.4±1.2和20.1±2.3;A549亲本细胞、L-MTX耐药细胞和D-MTX耐药细胞中:CD31+阳性率分别为55.5%±9.7%、32.9%±8.0%和91.9%±3.2%,差异有统计学意义(F=511.92,P=0.000);P-170阳性率分别为85.5%±4.6%、71.5%±8.2%和87.0%±8.9%,L-MTX耐药细胞P-170的阳性率低于D-MTX耐药细胞(t=6.13,P=0.002);3种细胞植入裸鼠成瘤后切片免疫标记CD34统计新生MVD,A549亲本细胞为3.6±1.2,L-MTX耐药细胞为7.2±1.7,D-MTX耐药细胞为55.9±11.9,D-MTX耐药细胞显著高于L-MTX耐药细胞(t=13.37,P=0.000).结论 MTX对映体诱导肺癌A549耐药细胞向血管内皮细胞分化能力不同,D-MTX耐药细胞经血管转移高于L-MTX耐药细胞,这提示MTX制剂中D型组分不能简单当成污染物而忽视,应尽量选用单一对映体的L-MTX药物制剂,减少D-MTX带来的副作用.